SD DNA Polymerase Protein
SD DNA Polymerase, concentrated for LAMP assay
Synonyms
SD DNA Polymerase; N/A; SD DNA Polymerase, concentrated for LAMP assay; SD Polymerase (for LAMP Assay); SD DNA Polymerase protein
Concentration
50 units/ul (50 units/ul recommended for LAMP) (varies by lot)
Activities
SD polymerase has 5'-3' polymerase and 5'-3' strand displacement activities. It does not have any exonuclease activity. The enzyme does A-overhangs.
Thermostability
SD polymerase is thermostable up to 93°C. In a denaturation step with LAMP or PCR amplification, 92°C is strongly recommended. Please find a standard. PCDR program suggested in this text under "PCDR".
Storage Buffer
10 mM K-phosphate buffer pH 7.0, 100 mM NaCI, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v)
10X Reaction Buffer
pH8.8, with Tween-20 and BSA added; add MgCl2 to a final concentration of 3- 3.5 mM before use.
Preparation and Storage
Store at -20°C in a constant temperature freezer.
Related Product Information for SD DNA Polymerase protein
SD polymerase is a variation of Taq polymerase with strong strand displacement activity and processivity. Two key changes turn ordinary Taq polymerase into a polymerase that handles the most Stringent Demands (SD): high GC content, strong secondary structure, long distance amplification, and the latest amplification assays. It often provides greater final product yields.
Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD polymerase is stable up to 93 °C. SD performs isothermal amplifications like LAMP; however, now you can add an initial denaturation step (92 oC) or hot-start for increased specificity. SD polymerase can perform long-distance PCR with extremely low concentrations of template. SD polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs.
Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD polymerase is stable up to 93 °C. SD performs isothermal amplifications like LAMP; however, now you can add an initial denaturation step (92 oC) or hot-start for increased specificity. SD polymerase can perform long-distance PCR with extremely low concentrations of template. SD polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs.
Product Categories/Family for SD DNA Polymerase protein
References
1. Claire L, et al. Polymerase chain displacement reaction. Biotechniques. 54: 93-97, 2013
2. Ignatov KB, et al. A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification. Biotechniques. 57: 81-87, 2014
2. Ignatov KB, et al. A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification. Biotechniques. 57: 81-87, 2014
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Product Notes
The SD DNA Polymerase (Catalog #AAA62068) is a Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SD DNA Polymerase, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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