WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells treated with EGF200ng/ml 30 min, using B-RAF (Phospho-Ser601) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit Raf-B Antibody | anti-BRAF antibody
Raf-B (phospho Ser601) Polyclonal Antibody (HRP)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Raf-B; N/A; Raf-B (phospho Ser601) Polyclonal Antibody (HRP); BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; anti-BRAF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
Phospho-Raf-B (S601) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S601.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA.
Applicable Applications for anti-BRAF antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser601. AA range:576-625.
Preparation and Storage
-20 degree C/1 year
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells treated with EGF200ng/ml 30 min, using B-RAF (Phospho-Ser601) Antibody. The lane on the right is blocked with the phospho peptide.)
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells treated with EGF200ng/ml 30 min, using B-RAF (Phospho-Ser601) Antibody. The lane on the right is blocked with the phospho peptide.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser601) Antibody. The picture on the right is blocked with the phospho peptide.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser601) Antibody. The picture on the right is blocked with the phospho peptide.)
WB (Western Blot)
(Western Blot analysis of 3T3 cells using Phospho-Raf-B (S601) Polyclonal Antibody.)
WB (Western Blot)
(Western Blot analysis of 3T3 cells using Phospho-Raf-B (S601) Polyclonal Antibody.)
Related Product Information for anti-BRAF antibody
B-Raf proto-oncogene, serine/threonine kinase(BRAF) Homo sapiens This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, no n-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008].
NCBI and Uniprot Product Information
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Product Notes
The BRAF (Catalog #AAA269104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raf-B (phospho Ser601) Polyclonal Antibody (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Raf-B can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BRAF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf-B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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