Rabbit anti-Human ALK (Anaplastic Lymphoma Kinase)/CD246 Antibody | anti-ALK antibody

ALK (Anaplastic Lymphoma Kinase)/CD246

Cat. Num. AAA214978

• Gene Names: ALK; CD246; NBLST3
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: ALK (Anaplastic Lymphoma Kinase)/CD246; N/A; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; anaplastic lymphoma receptor tyrosine kinase; mutant anaplastic lymphoma kinase; NBLST3; Tcrz; TFG/ALK; anti-ALK antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: ALK/3218R
• Specificity: The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1620

• Applicable Applications for anti-ALK antibody: IHC (Immunohistochemistry)
• Positive Control: Anaplastic Large Cell Lymphoma.
• Immunogen: Recombinant human ALK protein fragment (aa200-335)
• Cellular Localization: Cytoplasmic and Nuclear
• Hu-chromosome Location: 2p23
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified ALK-1 Recombinant Rabbit Monoclonal Antibody (ALK/3218R). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Anaplastic LC Lymphoma stained with ALK-1 Recombinant Rabbit Monoclonal Antibody (ALK/3218R).)

References

Pulford K, et al. Blood 1997;89:1394-404.

NCBI and Uniprot Product Information

• NCBI GI #: 29029632
• NCBI GeneID: 238
• NCBI Accession #: NP_004295.2
• NCBI GenBank Nucleotide #: NP_004295.2
• UniProt Accession #: Q9UM73
• Molecular Weight: 80kDa (hybrid); 200kDa (wild type)
• NCBI Official Full Name: ALK tyrosine kinase receptor isoform 1
• NCBI Official Synonym Full Names: ALK receptor tyrosine kinase
• NCBI Official Symbol: ALK
• NCBI Official Synonym Symbols: CD246; NBLST3
• NCBI Protein Information: ALK tyrosine kinase receptor
• UniProt Protein Name: ALK tyrosine kinase receptor
• UniProt Gene Name: ALK
• UniProt Entry Name: ALK_HUMAN