FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD8 antibody G10-1 (AAA72251). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of G10-1 (AAA72251-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit CD8 Antibody | anti-CD8 antibody
Anti-CD8 [G10-1], Rabbit IgG
Reactivity
Human, Macaca nemestrina
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Protein A affinity purified
Synonyms
CD8; N/A; Anti-CD8 [G10-1], Rabbit IgG; Anti-CD8 [G10-1]; cluster of differentiation 8; G10.1; Tp32; anti-CD8 antibody
Host
Rabbit
Reactivity
Human, Macaca nemestrina
Isotype
Rabbit IgG, Kappa
Clone Number
G10-1
Specificity
This antibody is specific for human CD8, and binds human CD8+ T cells. It also cross-reacts with non-human primate CD8, including that of Macaca nemestrina (Southern pig-tailed macaque).
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD8 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence)
Immunogen
Human CD8.
Origin Pub PMID
8929708
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD8 antibody G10-1 (AAA72251). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of G10-1 (AAA72251-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD8 antibody G10-1 (AAA72251). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of G10-1 (AAA72251-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-CD8 (AAA72251) G10-1 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of G10-1 (AAA72251-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72251-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-CD8 (AAA72251) G10-1 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of G10-1 (AAA72251-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72251-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD8 antibody
This antibody has been used to stain cells expressing CD8 by indirect immunofluorescence (Uckun et al, 1989) and in immunohistochemical analysis of aortic and iliac artery tissue from nonhuman primates (Masuda & Ross, 1989). It has also been employed in immunoprecipitation and gel electrophoresis experiments to investigate cell surface CD8 complexes (Ledbetter et al, 1985). A FITC-conjugated version of this antibody has been used to identify T cell populations in flow cytometry experiments (Chen et al, 2003). When conjugated to the ribosome-inhibitory protein ricin, this antibody acts as a potent CD8+ T cell-directed immunotoxin (Uckun et al, 1989).
Product Categories/Family for anti-CD8 antibody
References
Ledbetter et al. Covalent association between human thymus leukaemia-like antigens and CD8(Tp32) molecules. J Immunol. 1985 Jun;134(6):4250-4 PMID:3921621
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Product Notes
The CD8 (Catalog #AAA72251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD8 [G10-1], Rabbit IgG reacts with Human, Macaca nemestrina and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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