IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (AAA280628). Western blot was performed from the immunoprecipitate using Phospho- RAF1-S259 pAb (AAA280628) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.)
Rabbit anti-Human, Mouse Phospho-RAF1-S259 Antibody | anti-RAF1-S259 antibody
Phospho-RAF1-S259 Polyclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Affinity purification
Synonyms
Phospho-RAF1-S259; N/A; Phospho-RAF1-S259 Polyclonal Antibody ; RAF1; CMD1NN; CRAF; NS5; Raf-1; c-Raf; Raf-1 p roto-oncogene, serine/threonine kinase; anti-RAF1-S259 antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
648
Applicable Applications for anti-RAF1-S259 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic phosphorylated peptide around S259 of human RAF1 (NP_002871.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (AAA280628). Western blot was performed from the immunoprecipitate using Phospho- RAF1-S259 pAb (AAA280628) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (AAA280628). Western blot was performed from the immunoprecipitate using Phospho- RAF1-S259 pAb (AAA280628) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-RAF1-S259 antibody (AAA280628) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-RAF1-S259 antibody (AAA280628) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Related Product Information for anti-RAF1-S259 antibody
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Product Categories/Family for anti-RAF1-S259 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 73kDa
Calculated MW: 73kDa/75kDa
Calculated MW: 73kDa/75kDa
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN
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Product Notes
The RAF1-S259 raf1 (Catalog #AAA280628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-RAF1-S259 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-RAF1-S259 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RAF1-S259 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-RAF1-S259, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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