Rat CXCL1 ELISA Kit | CXCL1 elisa kit

Rat CXCL1 ELISA Kit

Cat. Num. AAA105581

• Gene Names: Cxcl1; Gro1; CINC-1
• Reactivity: Rat
• Applications: ELISA
• Synonyms: CXCL1; N/A; Rat CXCL1 ELISA Kit; GRO; GRO1; GROA; MGSA; SCYB1; Growth-regulated alpha protein; C-X-C motif chemokine 1; GRO-alpha(1-73); Melanoma growth stimulatory activity; Neutrophil-activating protein 3; NAP-3; CXCL1 elisa kit

• Reactivity: Rat
• Specificity: The Rat CXCL1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CXCL1 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 96
• Samples: Culture Supernatants, Serum, Plasma
• Sensitivity: < 8 pg/ml

• Applicable Applications for CXCL1 elisa kit: ELISA (Sandwich ELISA)
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for CXCL1 elisa kit

Background: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Principle of the Assay: The Rat CXCL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 13540652
• NCBI GeneID: 81503
• NCBI Accession #: NP_110472.1
• NCBI GenBank Nucleotide #: NM_030845.1
• UniProt Accession #: P14095
• Molecular Weight: 10,249 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: Gro1; CINC-1
• NCBI Protein Information: growth-regulated alpha protein; C-X-C motif chemokine 1; cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Cinc1; Gro; Scyb1; CINC-1
• UniProt Entry Name: GROA_RAT