Rat MMP9/MMP-9/CLG4B ELISA Kit | MMP9/MMP-9/CLG4B elisa kit

Rat MMP9/MMP-9/CLG4B ELISA Kit

Cat. Num. AAA258500

• Reactivity: Rat
• Synonyms: MMP9/MMP-9/CLG4B; N/A; Rat MMP9/MMP-9/CLG4B ELISA Kit; MMP-9 ELISA Kit, Rat; matrix metallopeptidase 9; MMP9/MMP-9/CLG4B elisa kit

• Reactivity: Rat
• Specificity: For the quantitative determination of Rat MMP9/MMP-9/CLG4B concentration in serum.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Serum
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 10.69pg/mL
• Conjugation: HRP
• Interference: Preparations of the following factors at 50ng/mL in a mid-range recombinant Rat MMP9 control were assayed for interference. No significant interference was observed.
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural Rat MMP9. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for MMP9/MMP-9/CLG4B elisa kit

This MMP-9 ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP-9 protein in Serum. It contains recombinant Rat MMP-9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis, and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases, and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and some transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodeling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent on the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. Besides, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.

References

St-Pierre Y, et al. (2003) Emerging features in the regulation of MMP-9 gene expression for the development of novel molecular targets and therapeutic strategies. Curr Drug Targets Inflamm Allergy. 2(3): 206-15.
St-Pierre Y, et al. (2004) Regulation of MMP-9 gene expression for the development of novel molecular targets against cancer and inflammatory diseases. Expert Opin Ther Targets. 8(5): 473-89.
Chakrabarti S, et al. (2005) Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology. Exp Lung Res. 31(6): 599-621.