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Application Data
(Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >468 pmol/min/ug, as measured under the described conditions)
MMP-9/CLG4B Active Protein | MMP9 active protein
Recombinant Human MMP-9/CLG4B Protein
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Purity
>97% by SDS-PAGE.
Synonyms
MMP-9/CLG4B; N/A; Recombinant Human MMP-9/CLG4B Protein; CLG4B; GELB; MANDP2; MMP-9; MMP9 active protein
Host
HEK293 Cells
Purity/Purification
>97% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Sequence Length
707
Endotoxin
< 0.1 EU/ug of the protein by LAL method.
Tags
C-His
Bio-Activity
1. Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration.
2. Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >468 pmol/min/ug, as measured under the described conditions.
2. Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >468 pmol/min/ug, as measured under the described conditions.
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycle
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles
Application Data
(Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >468 pmol/min/ug, as measured under the described conditions)
Application Data
(Recombinant Human MMP-9 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >468 pmol/min/ug, as measured under the described conditions)
Application Data
(Recombinant Human MMP-9 induces cell migration of the A549 cells. 1 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration.)
Application Data
(Recombinant Human MMP-9 induces cell migration of the A549 cells. 1 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration.)
SDS-PAGE
(Recombinant Human ISG15 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 100 kDa and 200 kDa, respectively.)
SDS-PAGE
(Recombinant Human ISG15 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 100 kDa and 200 kDa, respectively.)
SDS-PAGE
(Recombinant Human MMP-9/CLG4B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 100 kDa.)
SDS-PAGE
(Recombinant Human MMP-9/CLG4B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 100 kDa.)
Related Product Information for MMP9 active protein
Description: Recombinant Human MMP-9/CLG4B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Asp707 (Q279R)) of human MMP-9/CLG4B (Accession #NP_004985.2) fused with a 6xHis tag at the C-terminus.
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Product Categories/Family for MMP9 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
matrix metalloproteinase-9 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
MMP9
NCBI Official Synonym Symbols
GELB; CLG4B; MMP-9; MANDP2
NCBI Protein Information
matrix metalloproteinase-9
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Gene Name
MMP9
UniProt Synonym Gene Names
CLG4B; MMP-9; GELB
UniProt Entry Name
MMP9_HUMAN
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Product Notes
The MMP9 mmp9 (Catalog #AAA281425) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MMP-9/CLG4B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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