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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 2400-2450 of 14163 product results
Application Data
(Mass Spec analysis for Recombinant Mononucleosomes H3S10ph (EPL) Histone H3S10ph (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15306 Da. Determined mass = 15306 Da.)
Mononucleosomes H3S10ph, Recombinant Protein (Cat# AAA60168)
Application Data
(Recombinant Polynucleosomes (H3.1) protein activity assay. Results show that polynucleosomes are better substrates than octamers for NSD1-SET and NSD2-SET. Lanes 5-9 represent polynucleosomes that have been subjected to subsequent freeze-thaw cycles to demonstrate their stability. Activity was detected by fluorography.)
Polynucleosomes, Recombinant Protein (Cat# AAA59700)
Application Data
FLT3-ITD-W51, Recombinant Protein (Cat# AAA72985)
Application Data
MLL3, Recombinant Protein (Cat# AAA73023)
HIV-1 p24, Recombinant, Recombinant Protein (Cat# AAA58336)
Calbindin-1, Recombinant Protein (Cat# AAA38699)
Application Data
(NSD1-SET activity assay using Recombinant Nucleosomes as substrates. Recombinant Nucleosomes were used as substrates in an assay measuring the methyltransferase activity of NSD1-SET. Activity was detected by fluorography.)
NSD1-SET, Recombinant Protein (Cat# AAA59705)
Application Data
(Recombinant YEATS4 / GAS41 protein activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YEATS4, Recombinant Protein (Cat# AAA60172)
Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
HDAC4, Recombinant Protein (Cat# AAA59735)
Application Data
(Recombinant IDH2 (R172K) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH2 (R172K) protein in 200 ul reaction system (50 mM Tris-HCl pH 7.4, 150 mM NaCl. 10 mM MgCl2 and 0.03% BSA, room temp). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
IDH2, Recombinant Protein (Cat# AAA59788)
Collagen Type IV Alpha 3, Recombinant Protein (Cat# AAA38469)
Application Data
COMT, Recombinant Protein (Cat# AAA72975)
Application Data
PI3K, Recombinant Protein (Cat# AAA73057)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ALK (G1269A), Recombinant Protein (Cat# AAA73232)
CKMB, Recombinant Protein (Cat# AAA75420)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FAM20C, Recombinant Protein (Cat# AAA73575)
SDS-PAGE
(Purity: Sample purity data. For lot specific data, please refer to the respective datasheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKC beta I, Recombinant Protein (Cat# AAA73859)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
PAX8, Recombinant Protein (Cat# AAA48912)
Application Data
(HTRF assay for FAK (409-698) activity 1 uM TK substrate was incubated with different concentrations of FAK protein and 100 uM ATP in the reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
FAK, Recombinant Protein (Cat# AAA60153)
Application Data
(PRC2 EZH2 (A677G) complex activity assay. PRC2 EZH2(A677G) complex activity measured using an HMT assay. MALDI-TOF was used for detection.)
PRC2 EZH2, Recombinant Protein (Cat# AAA59656)
Application Data
(Recombinant DNMT1 protein activity assay Assay was performed using DNMT Activity / Inhibition Assay,. The substrate, AdoMet and DNMT1 were incubated for 1 hour at 37 degree C. The whole reactions were developed for 4 min. )
DNMT1, Recombinant Protein (Cat# AAA59661)
WB (Western Blot)
(250 ng (left lane) and 500 ng (right lane) were resolved on an SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with an anti-his antibody. A strong band of approximately 68 kDa was observed, along with a weaker band of approximately 25 kDa which represents an insignificant amount of degradation product.)
Ebola Virus VP40, Recombinant Protein (Cat# AAA224577)
Purified using proprietary chromatographic techniques
Application Data
TPM3-ALK, Recombinant Protein (Cat# AAA72946)
Application Data
KIAA1549-BRAF, Recombinant Protein (Cat# AAA72955)
Herpes Simplex Virus-1 (HSV-1) Glycoprotein D Glycosylated, Recombinant, Recombinant Protein (Cat# AAA58334)
Application Data
KAT6B, Recombinant Protein (Cat# AAA72999)
Application Data
ERK1, Recombinant Protein (Cat# AAA73017)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (N771-P772insH), Recombinant Protein (Cat# AAA73497)
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
SDS-PAGE
(Figure. SDS-PAGE)
Actin Related Protein 2/3 Complex Subunit 5 (ARPC5), Recombinant Protein (Cat# AAA162020)
Application Data
PDE4D, Recombinant Protein (Cat# AAA73102)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (L747S), Recombinant Protein (Cat# AAA73488)
CKMB, Recombinant Protein (Cat# AAA75580)
Application Data
(MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
MAOA, Recombinant Protein (Cat# AAA59723)
Application Data
(HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product. )
EHMT1, Recombinant Protein (Cat# AAA59731)
Application Data
(HTRF assay for YTHDF3 protein activity 3 uM biotinylated m6A ssDNA oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDF3 protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Recombinant PRC2 EZH2 (Y641C) complex activity assay. 1 uM H3K27me2 peptide was incubated with different concentration of PRC2 EZH2 (Y641C) complex in reaction buffer for 3 hour at room temperature. PRC2 EZH2(Y641C) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me3) was measured using H3K27me3-specific antibody.)
PRC2 EZH2, Recombinant Protein (Cat# AAA59654)
Adenovirus Antigen, Recombinant Protein (Cat# AAA58072)
SDS-PAGE
(DNMT1 protein gel. DNMT1 (2.8 ug) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.)
Application Data
(Recombinant IDH1 (R131C) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH1 (R132C) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
Application Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
IDH2, Recombinant Protein (Cat# AAA59785)
Application Data
(HTRF assay for EGFR (672-1210, L858R) protein activity 1 micro;M TK substrate was incubated with different concentrations of EGFR (672-1210, L858R) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE TK assay was used to detect the enzymatic activity.)
EGFR, Recombinant Protein (Cat# AAA60175)
Application Data
(The purity of AMPK was determined to be >75% by densitometry, approximate MW ~69kDa (A2), ~38 kDa (B1), and ~40kDa (G1).)
AMPK, Recombinant Protein (Cat# AAA73077)
Plasminogen Activator Inhibitor 1, Recombinant, Recombinant Protein (Cat# AAA58301)
Dengue Virus Type 4, Recombinant Protein (Cat# AAA58383)
HBcAg, Recombinant Protein (Cat# AAA75461)
LYVE1, Recombinant Protein (Cat# AAA75250)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.