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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 2450-2500 of 14163 product results
Application Data
BCR-RET, Recombinant Protein (Cat# AAA73112)
Application Data
NDR, Recombinant Protein (Cat# AAA73145)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316)
Tau-441 (P301L), Biotinylated, Recombinant Protein (Cat# AAA74048)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441 (P301S), Recombinant Protein (Cat# AAA74050)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441 (S352L), Recombinant Protein (Cat# AAA74053)
SARS-CoV-1 Spike RBD Coronavirus, Recombinant Protein (Cat# AAA268877)
Affinity purification chromatography.
HEPATITIS B 320 CORE ANTIGEN, Recombinant Protein (Cat# AAA50526)
Mycobacterium tuberculosis CFP10: ESAT6 Chimera, Recombinant Protein (Cat# AAA58047)
Nipah Virus G, Recombinant Protein (Cat# AAA58063)
Estrogen Receptor a, Recombinant Protein (Cat# AAA59634)
SDS-PAGE
(Purity: sample purity data. For lot-specific data please refer to the lot-specific datasheet.)
Application Data
(Recombinant HDAC2 activity assay. 1 uM H3K9ac peptide was incubated with different concentrations of HDAC2 protein in a reaction system for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added for 1 h at room temperature. HTRF assay was used for detection.)
HDAC2, Recombinant Protein (Cat# AAA59726)
Application Data
(MALDI-TOF for JARID1B / KDM5B (2-751) activity. 3.3 uM H3K4me3 (1-21 aa) peptide was incubated with 100 nM or 200 nM JARID1B / KDM5B (2-751) protein in reaction buffer for 2 hours at room temperature. MALDI-TOF was used for detection.Catalytic Ability: > 60 turnovers/ enzyme molecule)
JARID1B/KDM5B, Recombinant Protein (Cat# AAA59729)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
Cortactin, Recombinant Protein (Cat# AAA144144)
Application Data
p70S6Kb, Recombinant Protein (Cat# AAA73122)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
KIF5B-MET, Recombinant Protein (Cat# AAA73721)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PAK1/CDC42, Recombinant Protein (Cat# AAA73780)
NT-proBNP, Recombinant Protein (Cat# AAA78139)
Immunoaffinity purification followed by ion-exchange chromatography.
YELLOW FEVER VIRUS, Recombinant Protein (Cat# AAA50542)
Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
HDAC5, Recombinant Protein (Cat# AAA59739)
WB (Western Blot)
(Recombinant NSD2 (E1099K) activity assay.2 ug recombinant nucleosomes was incubated with 0 ug (-), 0.25 ug (+), 0.5 ug (++), 1 ug NSD2 (E1099K)(+++), respectively, in reaction buffer for 3 hours at room temperature. 2 ug histone octamer was used as a control substrate for NSD2 (E1099K) assay. Production (H3K36me2) was detected by Western blot.)
NSD2, Recombinant Protein (Cat# AAA59745)
WB (Western Blot)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
NSD3, Recombinant Protein (Cat# AAA59707)
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW 96kDa)
HSP70, Recombinant Protein (Cat# AAA72885)
Ornithine Aminotransferase, Recombinant Protein (Cat# AAA38652)
SDS-PAGE
(Figure. SDS-PAGE)
Connective Tissue Growth Factor (CTGF), Recombinant Protein (Cat# AAA150647)
DENGUE VIRUS TYPE 1 NS1 ANTIGEN, Recombinant Protein (Cat# AAA50549)
Application Data
(MALDI-TOF for PRC2 EZH2 (Y641N) activity.3.3 uM H3K27me2 peptide was incubated with 40 nM PRC2 EZH2 (Y641N) complex in reaction buffer for 3 hours at room temperature. Activity was detected by MALDI-TOF.Catalytic Ability:80 turnovers / enzyme molecule.)
PRC2 EZH2, Recombinant Protein (Cat# AAA59655)
SDS-PAGE
catalase (KatA), Recombinant Protein (Cat# AAA197064)
Ligand affinity chromatography
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MET (D1228Y), Recombinant Protein (Cat# AAA73697)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
Cytochrome P450 1A1 (CYP1A1), Recombinant Protein (Cat# AAA135506)
Gene Sequence
Filamin A Alpha, Recombinant Protein (Cat# AAA144292)
SDS-PAGE
DsbG, Recombinant Protein (Cat# AAA48495)
SDS-PAGE
STAT4, Recombinant Protein (Cat# AAA72890)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FLT3-ITD-NPOS, Recombinant Protein (Cat# AAA73566)
SDS-PAGE
(Purity: Sample purity data. for lot-specific data, please refer to the lot-specific datasheet.)
Bioactivity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet)
HBcAg, Recombinant Protein (Cat# AAA75372)
Application Data
KAT3A, Recombinant Protein (Cat# AAA72997)
Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JMJD1B/KDM3B, Recombinant Protein (Cat# AAA59678)
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SIRT1, Recombinant Protein (Cat# AAA59738)
COVID 19 Spike Coronavirus, Recombinant Protein (Cat# AAA77461)
Purified Full Length Recombinant Antigen (His-tag) Format.
cardiac troponin ITC complex, Recombinant Protein (Cat# AAA78145)
Gene Sequence
(Figure. Gene Sequencing (Extract))
Immunoglobulin M, Recombinant Protein (Cat# AAA150205)
Polymerase 1, Recombinant Protein (Cat# AAA38716)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.