At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
WB (Western Blot) (Figure 6 WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Spike RBD antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)
ELISA (Figure 5 ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike RBD antibody, 9087, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9087 does not distinguish between WT and Delta.)
WB (Western Blot) (Figure 4 WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, 9463, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant RBD protein (95-126), but not WT spike RBD protein (10-303), was specifically detected by Delta Variant spike L452R antibody (9463).)
ELISA (Figure 3 ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant RBD protein (95-126), but not WT spike RBD protein, was specifically detected by Delta Variant spike L452R antibody (9463).)
ELISA (Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.)
WB (Western Blot) (Figure 5 WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody.)
ELISA (Figure 4 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, and dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibodies.)
ELISA (Figure 3 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, and spike 156-157EF antibody, 9685, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689), while the corresponding WT antibody detected spike S1 protein of WT, but not delta variant.)
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE (Under DTT-reducing SDS-PAGE conditions, the Spike Protein RBD Domain migrates as a 32kDa protein (Lane 1).The binding of RBD-his protein to the ACE2 and the anti-2019 nCoV S1 mAb has been verified.)
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
What Are Recombinant Proteins?
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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