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10000 results for "Recombinant Proteins" - showing 9650-9700
WB (Western Blot)
(Figure 2. Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (AAA124699).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: rat thymus tissue lysates,Lane 5: human COLO-320 whole cell lysates,Lane 6: human HepG2 whole cell lysates,Lane 7: human 22RV1 whole cell lysates,Lane 8: human PANC-1 whole cell lysates,Lane 9: human SGC-7901 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase 4 at approximately 43KD. The expected band size for Caspase 4 is at 43KD.)
Caspase 4, Polyclonal Antibody (Cat# AAA124699)
Full Name
Anti-Caspase 4 Picoband antibody
Gene Names
Casp4; Caspl; ich-3; CASP-4; Casp11; CASP-11
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody.Ribonuclease 3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Ribonuclease 3 antibody.Ribonuclease 3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Ribonuclease 3 antibody.Ribonuclease 3 was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Ribonuclease 3 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Ribonuclease 3/RNASE3, Polyclonal Antibody (Cat# AAA124949)
Full Name
Anti-Ribonuclease 3/RNASE3 Antibody
Gene Names
RNASE3; ECP; RAF1; RNS3
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of IL33 using anti-IL33 antibody (AAA124548).IL33 was detected in paraffin-embedded section of human tonsil. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL33 Antibody (AAA124548) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IL33, Polyclonal Antibody (Cat# AAA124548)
Full Name
Anti-IL33 Picoband Antibody
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of LMAN1 using anti-LMAN1 antibody.LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.LMAN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.LMAN1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.LMAN1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
LMAN1, Polyclonal Antibody (Cat# AAA124954)
Full Name
Anti-LMAN1 Antibody
Gene Names
LMAN1; MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
OX40/TNFRSF4, Active Protein (Cat# AAA48994)
Full Name
Recombinant mouse OX40/TNFRSF4 protein
Gene Names
Tnfrsf4; Ox40; ACT35; CD134; Ly-70; Txgp1; TXGP1L
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Cerebrum Tissue; Primary Ab: 30ug/ml Mouse AntiHuman BNP AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Brain Natriuretic Peptide (BNP), Monoclonal Antibody (Cat# AAA151556)
Full Name
Monoclonal Antibody to Brain Natriuretic Peptide (BNP)
Gene Names
NPPB; BNP
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-K Cadherin/CDH6 antibody (AAA125770).Overlay histogram showing PC-3 cells stained with AAA125770 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-K Cadherin/CDH6 Antibody (AAA125770, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
K Cadherin/CDH6, Polyclonal Antibody (Cat# AAA125770)
Full Name
Anti-K Cadherin/CDH6 Antibody
Gene Names
CDH6; CAD6; KCAD
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using NKX3.1-Monospecific Mouse Monoclonal Antibody (NKX3.1/3350). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
NKX3.1, Monoclonal Antibody (Cat# AAA215102)
Full Name
NKX3.1 (Metastatic Prostate Adenocarcinoma Marker)
Gene Names
NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of A431 cells using anti-MEK2/MAP2K2 antibody (AAA125912).Overlay histogram showing A431 cells stained with AAA125912 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MEK2/MAP2K2 Antibody (AAA125912, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MEK2/MAP2K2, Monoclonal Antibody (Cat# AAA125912)
Full Name
Anti-MEK2/MAP2K2 Antibody (monoclonal, 2H4)
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of U-937 cells using anti- APPL/APPL1 antibody (AAA125923).Overlay histogram showing U-937 cells stained with AAA125923 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APPL/APPL1 Antibody (AAA125923, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
APPL/APPL1, Monoclonal Antibody (Cat# AAA125923)
Full Name
Anti-APPL/APPL1 Antibody (monoclonal, 5G11)
Gene Names
APPL1; APPL; DIP13alpha
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Colon Tissue; Primary Ab: 20ug/ml Mouse AntiHuman NB1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Neutrophil Specific Antigen 1 (NB1), Monoclonal Antibody (Cat# AAA151659)
Full Name
Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1)
Gene Names
CD177; NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-DDX6 antibody (AAA125932).Overlay histogram showing Hela cells stained with AAA125932 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-DDX6 Antibody (AAA125932, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DDX6, Monoclonal Antibody (Cat# AAA125932)
Full Name
Anti-DDX6 Antibody (monoclonal, 8G6)
Gene Names
DDX6; P54; RCK; HLR2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-PFN3 antibody (AAA127800).Overlay histogram showing HEL cells stained with AAA127800 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFN3 Antibody (AAA127800, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PFN3, Polyclonal Antibody (Cat# AAA127800)
Full Name
Anti-PFN3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-STAM2 antibody (AAA127380).Overlay histogram showing Hela cells stained with AAA127380 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAM2 Antibody (AAA127380, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STAM2, Polyclonal Antibody (Cat# AAA127380)
Full Name
Anti-STAM2 Antibody Picoband
Gene Names
STAM2; Hbp; STAM2A; STAM2B
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant sheep MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rsMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 degree C for 2 hours. The activated rsMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rsMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rsMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant sheep MMP1 is > 40 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA161685)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of 293T cells using anti-MEK5/MAP2K5 antibody (AAA125708).Overlay histogram showing 293T cells stained with AAA125708 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK5/MAP2K5 Antibody (AAA125708, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MEK5/MAP2K5, Polyclonal Antibody (Cat# AAA125708)
Full Name
Anti-MEK5/MAP2K5 Antibody
Gene Names
MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
Reactivity
Human, Monkey, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of NUFIP1 using anti-NUFIP1 antibody (AAA127631) and anti-Beta Tubulin antibody (M01857-3).NUFIP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-NUFIP1 Antibody (AAA127631) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
NUFIP1, Polyclonal Antibody (Cat# AAA127631)
Full Name
Anti-NUFIP1 Antibody Picoband
Gene Names
NUFIP1; NUFIP; bA540M5.1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of NOXA1 using anti-NOXA1 antibody (AAA127246).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA1 antigen affinity purified polyclonal antibody (#AAA127246) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOXA1 at approximately 60 kDa. The expected band size for NOXA1 is at 51 kDa.)
NOXA1, Polyclonal Antibody (Cat# AAA127246)
Full Name
Anti-NOXA1 Antibody Picoband
Gene Names
NOXA1; p51NOX; NY-CO-31; SDCCAG31
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-GLOD4 antibody (AAA128226).Overlay histogram showing HepG2 cells stained with AAA128226 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLOD4 Antibody (AAA128226, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GLOD4, Polyclonal Antibody (Cat# AAA128226)
Full Name
Anti-GLOD4 Antibody Picoband
Gene Names
GLOD4; HC71; CGI-150; C17orf25
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
hnRNP M3/4, Monoclonal Antibody (Cat# AAA213497)
Full Name
Mouse anti-hnRNP M3/4 Monoclonal Antibody, Purified [2A6-2H3]
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-ATG4B antibody (AAA127147).Overlay histogram showing RT4 cells stained with AAA127147 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4B Antibody (AAA127147, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ATG4B, Polyclonal Antibody (Cat# AAA127147)
Full Name
Anti-ATG4B Antibody Picoband
Gene Names
ATG4B; APG4B; AUTL1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Sarcalumenin/Srl antibody (AAA127468).Overlay histogram showing HEPA1-6 cells stained with AAA127468 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sarcalumenin/Srl Antibody (AAA127468, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Sarcalumenin/Srl, Polyclonal Antibody (Cat# AAA127468)
Full Name
Anti-Sarcalumenin/Srl Antibody Picoband
Gene Names
Srl; sar; AW108387; 9830004M20Rik
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using p90RSK mouse mAb.)
p90RSK, Monoclonal Antibody (Cat# AAA320397)
Full Name
p90RSK Mouse mAb
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Monkey
Applications
Immunoprecipitation, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of PC-3 cells using anti-MAP1S antibody (AAA127226).Overlay histogram showing PC-3 cells stained with AAA127226 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP1S Antibody (AAA127226, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAP1S, Polyclonal Antibody (Cat# AAA127226)
Full Name
Anti-MAP1S Antibody Picoband
Gene Names
MAP1S; MAP8; BPY2IP1; C19orf5; VCY2IP1; VCY2IP-1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RT4 cells using anti-NOP2 antibody (AAA127255).Overlay histogram showing RT4 cells stained with AAA127255 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOP2 Antibody (AAA127255, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NOP2, Polyclonal Antibody (Cat# AAA127255)
Full Name
Anti-NOP2 Antibody Picoband
Gene Names
NOP2; NOL1; p120; NSUN1; NOP120
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-BAP1 antibody (AAA126011).Overlay histogram showing MCF-7 cells stained with AAA126011 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP1 Antibody (AAA126011, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BAP1, Polyclonal Antibody (Cat# AAA126011)
Full Name
Anti-BAP1 Antibody Picoband
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of IQCE using anti-IQCE antibody (AAA127811) and anti-Beta Tubulin antibody (M01857-3).IQCE was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-IQCE Antibody (AAA127811) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Donkey Anti-Rabbit IgG and DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IQCE, Polyclonal Antibody (Cat# AAA127811)
Full Name
Anti-IQCE Antibody Picoband
Gene Names
IQCE; 1700028P05Rik
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of JK cells using anti-CD3D antibody (AAA127230).Overlay histogram showing JK cells stained with AAA127230 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD3D Antibody (AAA127230, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD3D, Polyclonal Antibody (Cat# AAA127230)
Full Name
Anti-CD3D Antibody Picoband
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ACE2 antibody (AAA127012).Overlay histogram showing HepG2 cells stained with AAA127012 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACE2 Antibody (AAA127012, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ACE2, Polyclonal Antibody (Cat# AAA127012)
Full Name
Anti-ACE2 Antibody Picoband
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-GLYAT antibody (AAA127587).Overlay histogram showing RT4 cells stained with AAA127587 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLYAT Antibody (AAA127587, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GLYAT, Polyclonal Antibody (Cat# AAA127587)
Full Name
Anti-GLYAT Antibody Picoband
Gene Names
GLYAT; CAT; GAT; ACGNAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of TDP2 using anti-TDP2 antibody (AAA126177).TDP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TDP2 Antibody (AAA126177) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
TDP2, Polyclonal Antibody (Cat# AAA126177)
Full Name
Anti-TDP2 Antibody Picoband
Gene Names
TDP2; EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-Nr0b2 antibody (AAA126338).Overlay histogram showing ANA-1 cells stained with AAA126338 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nr0b2 Antibody (AAA126338, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Nr0b2, Polyclonal Antibody (Cat# AAA126338)
Full Name
Anti-Nr0b2 Antibody Picoband
Gene Names
Nr0b2; SHP; Shp1; SHP-1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U87 cells using anti-SCYL2 antibody (AAA126526).Overlay histogram showing U87 cells stained with AAA126526 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCYL2 Antibody (AAA126526, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SCYL2, Polyclonal Antibody (Cat# AAA126526)
Full Name
Anti-SCYL2 Antibody Picoband
Gene Names
SCYL2; CVAK104
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U87 cells using anti-ATP6V1A antibody (AAA126575).Overlay histogram showing U87 cells stained with AAA126575 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP6V1A Antibody (AAA126575, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP6V1A, Polyclonal Antibody (Cat# AAA126575)
Full Name
Anti-ATP6V1A Antibody Picoband
Gene Names
ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of ANA-1 cells using anti-Cdk2 antibody (AAA126862).Overlay histogram showing ANA-1 cells stained with AAA126862 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cdk2 Antibody (AAA126862, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Cdk2, Monoclonal Antibody (Cat# AAA126862)
Full Name
Anti-Cdk2 Antibody Picoband (monoclonal, 5B12D1)
Gene Names
CDK2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of MOLT-4 cells using anti-ACE antibody (AAA126974).Overlay histogram showing MOLT-4 cells stained with AAA126974 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ACE Antibody (AAA126974, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ACE, Polyclonal Antibody (Cat# AAA126974)
Full Name
Anti-ACE Antibody Picoband
Gene Names
ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Standard Curve (Sample)
IL-1beta, CLIA Kit (Cat# AAA318570)
Full Name
IL-1beta (Murine) LumiAb ELISA Kit
Gene Names
Il1b; Il-1b; IL-1beta
Reactivity
Mouse
Pricing
Bioactivity
(Figure 2. Cell apoptosis of A549 cells after stimulated with recombinant dog IFNb.)
Interferon Beta (IFNb), Active Protein (Cat# AAA153001)
Full Name
Active Interferon Beta (IFNb)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Standard Curve (Sample)
MMP2, ELISA Kit (Cat# AAA105958)
Full Name
Rat MMP2 ELISA Kit
Reactivity
Rat
Applications
Sandwich ELISA
Pricing
Standard Curve (Sample)
IL-33, ELISA Kit (Cat# AAA106018)
Full Name
Mouse IL-33 ELISA Kit
Gene Names
Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
Reactivity
Mouse
Applications
Sandwich ELISA
Pricing
Standard Curve (Sample)
IGFBP2, ELISA Kit (Cat# AAA105687)
Full Name
Human IGFBP2 ELISA Kit
Gene Names
IGFBP2; IBP2; IGF-BP53
Reactivity
Human
Applications
Sandwich ELISA
Pricing
Bioactivity
(Detects Human IL15 Antibody in indirect ELISAs.)
IL15, Recombinant Protein (Cat# AAA120479)
Full Name
Recombinant Human IL15, C-His
Gene Names
IL15; IL-15
Applications
Western Blot
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
CD9, Recombinant Protein (Cat# AAA48389)
Full Name
Recombinant human CD9 protein
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Applications
SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using MSN antibody.)
MSN, Polyclonal Antibody (Cat# AAA37002)
Full Name
MSN Polyclonal Antibody
Gene Names
MSN; HEL70
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
ICC (Immunocytochemistry)
(Immunocytochemistry of CTRP7 in 293 cells with CTRP7 antibody at 10 ug/mL.)
CTRP7, Polyclonal Antibody (Cat# AAA305513)
Full Name
CTRP7 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Affinity chromatography purified via peptide column
Pricing
Bioactivity
(Detects Human CD5 Antibody (5D7) in indirect ELISAs.)
CD5, Recombinant Protein (Cat# AAA120437)
Full Name
Recombinant Human CD5, C-His
Gene Names
CD5; T1; LEU1
Applications
Western Blot
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(The OmniKine Human Angiopoietin-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Angiopoietin-1 proteins within the range of 157-10000 pg/ml.)
Angiopoietin-1, ELISA Kit (Cat# AAA315780)
Full Name
Angiopoietin-1 (Human) OmniKine ELISA Kit
Gene Names
ANGPT1; AGP1; AGPT; ANG1
Reactivity
Human
Pricing
Alpha-1 Antitrypsin, Native Protein (Cat# AAA38498)
Full Name
Human Alpha-1 Antitrypsin
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
Amino Acid Info
Serum Amyloid A (SAA), Recombinant Protein (Cat# AAA153399)
Full Name
Recombinant Serum Amyloid A (SAA)
Gene Names
Saa1; Saa2; Saa-1
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Interleukin 35 (IL35), Recombinant Protein (Cat# AAA161989)
Full Name
Recombinant Interleukin 35 (IL35)
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing