Search results for "DNA RNA"

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WB (Western Blot) (WB Suggested Anti-NR1I2 antibody Titration: 1 ug/mLSample Type: Human heart)

NR1I2, Polyclonal Antibody (Cat# AAA23452)

Full Name

NR1I2 antibody - N-terminal region

Gene Names

NR1I2; BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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FOXO3A, Polyclonal Antibody (Cat# AAA31313)

Full Name

Acetyl-FOXO3A (Lys271) Antibody

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen using NAT10 Rabbit mAb (AAA28499) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

NAT10, Monoclonal Antibody (Cat# AAA28499)

Full Name

NAT10 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-NFATC3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysate)

NFATC3, Polyclonal Antibody (Cat# AAA23612)

Full Name

NFATC3 antibody - N-terminal region

Gene Names

NFATC3; NFAT4; NFATX; NF-AT4c

Reactivity

Cow, Dog, Horse, Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (AAA28170) at dilution of 1:100. Blue: DAPI for nuclear staining.)

FUS, Polyclonal Antibody (Cat# AAA28170)

Full Name

FUS Polyclonal Antibody

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-GLI2 antibody (AAA19737).Overlay histogram showing U20S cells stained with AAA19737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (AAA19737, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of GLI2 using anti-GLI2 antibody (AAA19737) and anti-Beta Tubulin antibody (M01857-3).GLI2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (#AAA19737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 220 kDa.)

GLI2, Polyclonal Antibody (Cat# AAA19737)

Full Name

Anti-GLI2 Antibody Picoband

Gene Names

GLI2; HPE9; PHS2; THP1; THP2

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with FUS/TLS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

FUS/TLS, Monoclonal Antibody (Cat# AAA30253)

Full Name

FUS/TLS Antibody

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)

PLD6, Polyclonal Antibody (Cat# AAA28684)

Full Name

PLD6 Antibody (Center)

Gene Names

PLD6; ZUC

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

Poly (ADP-Ribose), ELISA Kit (Cat# AAA11446)

Full Name

Poly (ADP-Ribose) ELISA Kit

Pricing

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WB (Western Blot) (WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human liver)

FOXA1, Polyclonal Antibody (Cat# AAA23415)

Full Name

FOXA1 antibody - C-terminal region

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rat, Yeast, Zebrafish

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-RXRA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T)

RXRA, Polyclonal Antibody (Cat# AAA23431)

Full Name

RXRA antibody - N-terminal region

Gene Names

RXRA; NR2B1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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LDHB, Polyclonal Antibody (Cat# AAA23530)

Full Name

LDHB Antibody - C-terminal region

Gene Names

LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-RBM47 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: NCI-H226)

RBM47, Polyclonal Antibody (Cat# AAA23486)

Full Name

RBM47 antibody - middle region

Gene Names

RBM47; NET18

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-PRDM15 antibody (AAA19987).Overlay histogram showing A431 cells stained with AAA19987 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM15 Antibody (AAA19987, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PRDM15 using anti-PRDM15 antibody (AAA19987).PRDM15 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM15 Antibody (AAA19987) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM15 antigen affinity purified polyclonal antibody (#AAA19987) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (62 kDa.)

PRDM15, Polyclonal Antibody (Cat# AAA19987)

Full Name

Anti-PRDM15 Antibody Picoband

Gene Names

PRDM15; PFM15; ZNF298; C21orf83

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-MMS19 antibody (AAA19865).Overlay histogram showing Hela cells stained with AAA19865 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMS19 Antibody (AAA19865, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MMS19 using anti-MMS19 antibody (AAA19865).MMS19 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMS19 antigen affinity purified polyclonal antibody (#AAA19865) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMS19 at approximately 113 kDa. The expected band size for MMS19 is at 113 kDa.)

MMS19, Polyclonal Antibody (Cat# AAA19865)

Full Name

Anti-MMS19 Antibody Picoband

Gene Names

MMS19; MET18; MMS19L; hMMS19

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-GNAI1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain)

GNAI1, Polyclonal Antibody (Cat# AAA23558)

Full Name

GNAI1 antibody - middle region

Gene Names

GNAI1; Gi

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Yeast, Zebrafish

Applications

Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-RXRA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateRXRA is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)

RXRA, Polyclonal Antibody (Cat# AAA23509)

Full Name

RXRA antibody - N-terminal region

Gene Names

RXRA; NR2B1

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.Calnexin Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)

CHOP, Monoclonal Antibody (Cat# AAA30832)

Full Name

CHOP Mouse Monoclonal Antibody (2B1)

Gene Names

DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

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WB (Western Blot) (WB Suggested Anti-IGF2BP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysateIGF2BP2 is supported by BioGPS gene expression data to be expressed in HT1080)

IGF2BP2, Polyclonal Antibody (Cat# AAA23481)

Full Name

IGF2BP2 antibody - middle region

Gene Names

IGF2BP2; IMP2; IMP-2; VICKZ2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast

Applications

Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-ADRB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)

ADRB1, Polyclonal Antibody (Cat# AAA23455)

Full Name

ADRB1 antibody - middle region

Gene Names

ADRB1; RHR; B1AR; ADRB1R; BETA1AR

Reactivity

Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MFAP3 antibody (AAA19989).Overlay histogram showing 293T cells stained with AAA19989 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFAP3 Antibody (AAA19989, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MFAP3 using anti-MFAP3 antibody (AAA19989).MFAP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFAP3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFAP3 at approximately 45-50 kDa. The expected band size for MFAP3 is at 40 kDa.)

MFAP3, Polyclonal Antibody (Cat# AAA19989)

Full Name

Anti-MFAP3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-SIRT4 antibody Titration: 1 ug/mLSample Type: Human Raji)

SIRT4, Polyclonal Antibody (Cat# AAA23411)

Full Name

SIRT4 antibody - middle region

Gene Names

SIRT4; SIR2L4

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FUS recombinant monoclonal antibody (AAA23798 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-FUS recombinant monoclonal antibody .)

FUS, Monoclonal Recombinant Antibody (Cat# AAA23798)

Full Name

Rabbit anti-FUS Recombinant Monoclonal Antibody [BLR023E]

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry

Purity

Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-HNRPA0 Antibody Titration: 0.625ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)

HNRPA0, Polyclonal Antibody (Cat# AAA23482)

Full Name

HNRPA0 antibody - middle region

Gene Names

HNRNPA0; HNRPA0

Reactivity

Cow, Dog, Human, Pig

Applications

Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using POLR2A antibody at dilution of 1:100 (40x lens).)

POLR2A, Polyclonal Antibody (Cat# AAA28236)

Full Name

POLR2A Polyclonal Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

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WB (Western Blot) (WB Suggested Anti-HGF Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that HGF is expressed in HEK293T)

HGF, Polyclonal Antibody (Cat# AAA23517)

Full Name

HGF antibody - N-terminal region

Gene Names

HGF; SF; HGFB; HPTA; F-TCF; DFNB39

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-PUF60 Antibody Titration: 0.2-1 ug/mlPositive Control: Hela cell lysatePUF60 is supported by BioGPS gene expression data to be expressed in HeLa)

PUF60, Polyclonal Antibody (Cat# AAA23485)

Full Name

PUF60 antibody - C-terminal region

Gene Names

PUF60; FIR; VRJS; RoBPI; SIAHBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 15 kDa isoform is identified, and a second isoform of 18 kDa is also present in some samples.)

PFN1, Polyclonal Antibody (Cat# AAA23532)

Full Name

PFN1 antibody - N-terminal region

Gene Names

PFN1; ALS18

Reactivity

Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

Western Blot

Purity

Affinity Purified

Pricing

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Application Data (Source:Arthrobacter luteus)

Zymolyase 20T, Enzyme (Cat# AAA14844)

Full Name

Zymolyase 20T (Lyticase, Yeast Lytic Enzyme)

Pricing

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WB (Western Blot) (WB Suggested Anti-SNRP70 antibody Titration: 1 ug/mLSample Type: Human Raji)

SNRP70, Polyclonal Antibody (Cat# AAA23477)

Full Name

SNRP70 antibody - C-terminal region

Gene Names

SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-TLS/FUS antibody (AAA19406).Overlay histogram showing U87 cells stained with AAA19406 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLS/FUS Antibody (AAA19406, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TLS/FUS, Polyclonal Antibody (Cat# AAA19406)

Full Name

Anti-TLS/FUS Antibody Picoband

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-HOXB2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

HOXB2, Polyclonal Antibody (Cat# AAA23444)

Full Name

HOXB2 antibody - N-terminal region

Gene Names

HOXB2; K8; HOX2; HOX2H; Hox-2.8

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

ROR-gamma/RORC, Monoclonal Antibody (Cat# AAA23951)

Full Name

ROR-gamma/RORC (RAR-related Orphan Receptor C)

Gene Names

RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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WB (Western Blot) (WB Suggested Anti-SGCE Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysateSGCE is supported by BioGPS gene expression data to be expressed in 721_B)

SGCE, Polyclonal Antibody (Cat# AAA23523)

Full Name

SGCE antibody - N-terminal region

Gene Names

SGCE; ESG; DYT11; epsilon-SG

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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ICC (Immunocytochemistry) (ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

p54nrb, Monoclonal Antibody (Cat# AAA30509)

Full Name

p54nrb Antibody

Gene Names

NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

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EXOSC8, Polyclonal Antibody (Cat# AAA19607)

Full Name

Anti-EXOSC8 Antibody Picoband

Gene Names

EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-IGF2BP1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)

IGF2BP1, Polyclonal Antibody (Cat# AAA23578)

Full Name

IGF2BP1 antibody - N-terminal region

Gene Names

IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-HNRPUL1 Antibody Titration: 0.25ug/mlELISA Titer: 1:312500Positive Control: Raji cell lysateHNRNPUL1 is supported by BioGPS gene expression data to be expressed in Raji)

HNRPUL1, Polyclonal Antibody (Cat# AAA23483)

Full Name

HNRPUL1 antibody - C-terminal region

Gene Names

HNRNPUL1; E1BAP5; E1B-AP5; HNRPUL1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

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WB (Western Blot) (WB Suggested Anti-C19orf2 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Muscle)

C19orf2, Polyclonal Antibody (Cat# AAA23539)

Full Name

C19orf2 antibody - middle region

Gene Names

URI1; RMP; URI; NNX3; C19orf2; PPP1R19

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of U87 cells using anti-RBMS1 antibody (AAA19911).Overlay histogram showing U87 cells stained with AAA19911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBMS1 Antibody (AAA19911, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of RBMS1 using anti-RBMS1 antibody (AAA19911).RBMS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS1 antigen affinity purified polyclonal antibody (#AAA19911) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBMS1 at approximately 50 kDa. The expected band size for RBMS1 is at 45 kDa.)

RBMS1, Polyclonal Antibody (Cat# AAA19911)

Full Name

Anti-RBMS1 Antibody Picoband

Gene Names

RBMS1; YC1; MSSP; SCR2; HCC-4; MSSP-1; MSSP-2; MSSP-3; C2orf12

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-CREB3L2 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: Jurkat cell lysate)

CREB3L2, Polyclonal Antibody (Cat# AAA23437)

Full Name

CREB3L2 antibody - N-terminal region

Gene Names

CREB3L2; BBF2H7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRB antibody (AAA19985).Overlay histogram showing HCT116 cells stained with AAA19985 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRB Antibody (AAA19985, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of SRPRB using anti-SRPRB antibody (AAA19985).SRPRB was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRB Antibody (AAA19985) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRB Antibody (AAA19985) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRB antigen affinity purified polyclonal antibody (#AAA19985) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRPRB at approximately 30 kDa. The expected band size for SRPRB is at 30 kDa.)

SRPRB, Polyclonal Antibody (Cat# AAA19985)

Full Name

Anti-SRPRB Antibody Picoband

Gene Names

SRPRB; APMCF1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-PRPF18 antibody (AAA19988).Overlay histogram showing THP-1 cells stained with AAA19988 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF18 Antibody (AAA19988, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PRPF18 using anti-PRPF18 antibody (AAA19988).PRPF18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (#AAA19988) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (42 kDa.)

PRPF18, Polyclonal Antibody (Cat# AAA19988)

Full Name

Anti-PRPF18 Antibody Picoband

Gene Names

PRPF18; PRP18; hPrp18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-C18orf22/RBFA antibody (AAA19984).Overlay histogram showing MCF-7 cells stained with AAA19984 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C18orf22/RBFA Antibody (AAA19984, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (AAA19984).C18orf22/RBFA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MOLT-4 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C18orf22/RBFA antigen affinity purified polyclonal antibody (#AAA19984) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C18orf22/RBFA at approximately 40 kDa. The expected band size for C18orf22/RBFA is at 38 kDa.)

C18orf22/RBFA, Polyclonal Antibody (Cat# AAA19984)

Full Name

Anti-C18orf22/RBFA Antibody Picoband

Gene Names

RBFA; HsT169; C18orf22

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Jurkat cells using anti-SUCLG1 antibody (AAA19867).Overlay histogram showing Jurkat cells stained with AAA19867 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG1 Antibody (AAA19867, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SUCLG1 using anti-SUCLG1 antibody (AAA19867).SUCLG1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (#AAA19867) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 35 kDa. The expected band size for SUCLG1 is at 36 kDa.)