Standard Curve (Sample)
Human TNF alpha ELISA Kit | TNF elisa kit
Human TNF alpha ELISA Kit
Standard Curve (Sample)
Standard Curve (Sample)
Principle of the Assay: The Human TNF alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF alpha in Cell Culture Supernatants, Serum, Plasma, Urine, Tissue Homogenates. This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Product Notes
The Human TNF tnf (Catalog #AAA17896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17896 ELISA Kit recognizes Human TNF. AAA Biotech's TNF alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
Can this TNF-α ELISA kit detect low levels of TNF in serum or plasma?
Yes, TNF-α ELISA kits are designed to detect low-level TNF concentrations in serum and plasma. Standard TNF-α ELISA kits typically achieve sensitivity in the range of 1.9–4.3 pg/mL, allowing reliable quantification of basal TNF levels in healthy samples as well as elevated levels in inflammatory states. The sandwich ELISA format with pre-coated capture antibodies enables accurate measurement across a broad detection range.
What human sample types work best with this TNF ELISA kit?
TNF-α ELISA kits work optimally with serum, plasma (EDTA, citrate, or heparin), cell culture supernatants, and tissue homogenates. For best results with serum and plasma, samples should be collected by standard phlebotomy, centrifuged within 30 minutes, and either assayed immediately or stored at ≤-20°C in aliquots to avoid degradation and minimize sample handling artifacts.
Do samples need dilution before running the TNF assay?
Yes, serum and plasma samples typically require dilution before TNF-α ELISA analysis. A minimum 2-fold dilution is commonly recommended to reduce matrix interference from blood components, improving assay accuracy and precision. Pre-testing is advised to determine the optimal dilution factor based on the expected TNF concentration in your specific samples.
How long does the TNF-α ELISA take from start to finish?
Standard TNF-α ELISA assays typically require 3.5–5 hours from sample preparation to final reading. However, optimized rapid ELISA formats, such as single-wash SimpleStep ELISA protocols, can complete the assay in approximately 90 minutes, depending on the kit design and incubation parameters used.
Does hemolysis or lipemia affect TNF measurement?
Yes, both hemolysis and lipemia can interfere with TNF-α measurement. Hemolysis causes positive bias due to hemoglobin absorbance at 450 nm (the typical ELISA reading wavelength), while lipemia can cause either positive or negative bias depending on triglyceride concentration. It is recommended to avoid hemolysed and lipemic samples or to use samples with minimal hemolysis/lipemia to ensure accurate results.
Is this kit suitable for inflammation or cytokine-release studies?
Yes, TNF-α ELISA is ideal for inflammation and cytokine-release studies. TNF-α is a key pro-inflammatory cytokine marker elevated in immune responses, infections, and cytokine release syndrome (CRS). The kit is well-suited for measuring TNF-α dynamics in inflammation models, immune activation studies, and clinical research investigating immune activation or systemic inflammation.
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