Search results for "Recombinant Proteins"

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Sequence

Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161902)

Full Name

Active Carbonic Anhydrase IV (CA4)

Gene Names

Car4; Ca4

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

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Ebola Virus NP, Recombinant Protein (Cat# AAA24009)

Full Name

Ebola Virus NP protein

Applications

Western Blot

Purity

> 90% pure
Purified by chromatograpy from Zaire stain, this nucleoprotein is phosphorylated and O-glycosolated by the host organisms cells.

Pricing

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FCM (Flow Cytometry) (Figure 17. Flow Cytometry analysis of U251 cells using anti-SAMD9L antibody (AAA19973).Overlay histogram showing U251 cells stained with AAA19973 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAMD9L Antibody (AAA19973, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 15. IHC analysis of SAMD9L using anti-SAMD9L antibody (AAA19973).SAMD9L was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SAMD9L Antibody (AAA19973) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMD9L antigen affinity purified polyclonal antibody (#AAA19973) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAMD9L at approximately 200 kDa. The expected band size for SAMD9L is at 185 kDa.)

SAMD9L, Polyclonal Antibody (Cat# AAA19973)

Full Name

Anti-SAMD9L Antibody Picoband

Gene Names

SAMD9L; UEF1; DRIF2; C7orf6

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

DnaK, Monoclonal Antibody (Cat# AAA14655)

Full Name

DnaK

Reactivity

E Coli

Applications

Western Blot

Purity

Affinity Purified
Purified by Protein G affinity chromatography.

Pricing

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24421)

Full Name

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) APC

Gene Names

AFP; AFPD; FETA; HPAFP

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence - anti-CTSD Ab at 1/100 dilution in RAW264.7 cells; cells were fixed with PFA and permeabilized with 0.05% saponin)

Cathepsin D, Polyclonal Antibody (Cat# AAA13859)

Full Name

Cathepsin D Polyclonal Antibody

Gene Names

CTSD; CPSD; CLN10; HEL-S-130P

Reactivity

Reacts against human, rat, mouse, canine and monkey proteins.

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry

Pricing

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WB (Western Blot) (Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).)

Calnexin, Monoclonal Antibody (Cat# AAA23894)

Full Name

Calnexin (Endoplasmic Reticulum Marker)

Gene Names

CANX; CNX; P90; IP90

Reactivity

Human.
Does not react with rat. Others not known.

Applications

Western Blot, Immunohistochemistry

Pricing

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Application Data (ELISA: Immobilize various types of SARS proteins at concentration of 2g/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100g/ml, 10g/ml and 1g/ml. It shows the SARS-CoV-2-N Antibody (AAA27038) is specific for SARS-CoV-2-N protein, without any cross- reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.)

COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA27038)

Full Name

Human Novel Coronavirus Nucleoprotein (N) (1-419aa) Antibody

Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Applications

Colloidal Gold Immunochromatographic Assay

Purity

Affinity-chromatography

Pricing

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ICAM-1/CD54, Monoclonal Antibody (Cat# AAA28578)

Full Name

ICAM-1/CD54 Rabbit PolymAb

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of THP-1 cells using FRS2 Rabbit PolymAb® (AAA28559) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

FRS2, Monoclonal Antibody (Cat# AAA28559)

Full Name

FRS2 Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-FAM40B/STRIP2 antibody (AAA19969).Overlay histogram showing U87 cells stained with AAA19969 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM40B/STRIP2 Antibody (AAA19969, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of FAM40B/STRIP2 using anti-FAM40B/STRIP2 antibody (AAA19969).FAM40B/STRIP2 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM40B/STRIP2 Antibody (AAA19969) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hacat whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human SiHa whole cell lysates,Lane 5: rat testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM40B/STRIP2 antigen affinity purified polyclonal antibody (#AAA19969) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FAM40B/STRIP2 at approximately 100 kDa. The expected band size for FAM40B/STRIP2 is at 95 kDa.)

FAM40B/STRIP2, Polyclonal Antibody (Cat# AAA19969)

Full Name

Anti-FAM40B/STRIP2 Antibody Picoband

Gene Names

STRIP2; FAM40B; FAR11B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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HIV-1 gp140, Recombinant Protein (Cat# AAA13754)

Full Name

HIV-1 gp140 (Clade C) protein (amino acid 24-680), trimer majority

Applications

Western Blot

Pricing

FGF2, Polyclonal Antibody (Cat# AAA11513)

Full Name

Anti-human FGF2 antibody

Gene Names

FGF2; BFGF; FGFB; FGF-2; HBGF-2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse intestine tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

CD66a/CEACAM1, Monoclonal Antibody (Cat# AAA28579)

Full Name

CD66a/CEACAM1 Rabbit PolymAb

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSX1 protein using SSX1 antibody)

SSX1, Monoclonal Antibody (Cat# AAA106681)

Full Name

SSX1 antibody

Gene Names

SSX1; SSRC; CT5.1

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

SSX1 antibody was purified by affinity chromatography.

Pricing

SMAD4, Monoclonal Antibody (Cat# AAA24363)

Full Name

SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (AP)

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

HIV-1 tat, Recombinant Protein (Cat# AAA14180)

Full Name

HIV-1 TAT antigen (recombinant) full length 14 kDa

Purity

> 95% pure
Purity of proteins is evaluated by SDS-PAGE by measuring optimal density at 280 nm.

Pricing

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ICC (Immunocytochemistry) (Confocal imaging of C6 cells using GLDC Rabbit PolymAb® (AAA28583, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

GLDC, Monoclonal Antibody (Cat# AAA28583)

Full Name

GLDC Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

COVID 19 Nucleocapsid (NP) N fragment protein (NX) Coronavirus, Recombinant Protein (Cat# AAA27950)

Full Name

2019-nCov Nucleocapsid protein N fragment protein (NX)

Applications

SDS-Page

Purity

Purity: >95% by SDS-page
Purification: Ni column

Pricing

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TOM20, Monoclonal Antibody (Cat# AAA28304)

Full Name

TOM20 Rabbit mAb

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

CD163, Recombinant Protein (Cat# AAA10873)

Full Name

Recombinant Porcine CD163

Purity

Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Pricing

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IHC (Immunohistochemistry) (Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)

S100A9, Polyclonal Antibody (Cat# AAA11645)

Full Name

Anti-S100A9 Antibody

Gene Names

S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen Affinity Purified

Pricing

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Bioactivity (Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant pig AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant pig AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant pig AP is >600 U/mg.)

Aprotinin (AP), Active Protein (Cat# AAA161813)

Full Name

Active Aprotinin (AP)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)

Full Name

Laminin Receptor/RPSA (Marker of Metastatic Potential)

Gene Names

RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4

Reactivity

Human. Others not known.

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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Ki67, Monoclonal Antibody (Cat# AAA28581)

Full Name

Ki67 Rabbit PolymAb

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

Hemoglobin A1c, Native Protein (Cat# AAA10832)

Full Name

Human Hemoglobin A1c

Purity

Greater than 96.0%.

Pricing

Fascin, Recombinant Protein (Cat# AAA10880)

Full Name

Recombinant Human Fascin

Gene Names

FSCN1; HSN; SNL; p55; FAN1

Purity

Greater than 95.0% as determined by SDS-PAGE.

Pricing

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HA-Tag, Monoclonal Antibody (Cat# AAA27030)

Full Name

HA-Tag Monoclonal Antibody

Reactivity

All

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation

Purity

>95%, Protein A purified

Pricing

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant human CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161776)

Full Name

Active Carbonic Anhydrase II (CA2)

Gene Names

CA2; CAC; CAII; Car2; CA-II; HEL-76

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

HA (H9N2)A/Hong Kong/1073/99), Recombinant Protein (Cat# AAA13761)

Full Name

HA (H9N2)A/Hong Kong/1073/99) (aa 19-530)

Applications

Western Blot

Purity

> 95% pure by 10% SDS-PAGE gel

Pricing

Hirudin, Active Protein (Cat# AAA10836)

Full Name

Recombinant Hirudin

Purity

Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27700)

Full Name

VPS35 Antibody, Clone 11H10

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using GSDME antibody (AAA28161) at dilution of 1:50. Blue: DAPI for nuclear staining.)

DFNA5, Polyclonal Antibody (Cat# AAA28161)

Full Name

DFNA5

Gene Names

DFNA5; ICERE-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Pricing

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Bioactivity (Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors. Human growth hormone is a 191 amino acid single-chain polypeptide produced via the anterior pituitary of the brain in the acidophilic, somatotrophic cells. Its production is tightly regulated through several complex feedback mechanisms in response to stress, exercise, nutrition, sleep, and growth hormone itself. Annexin A2 (ANXA2) has been shown to play multiple roles in growth, development, metabolism and ANXA2 is a high affinity receptor for GH. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GH and recombinant human ANXA2. Briefly, GH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ANXA2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GH and recombinant human ANXA2 was shown in Figure 1, the EC50 for this effect is 2.1 ug/mL.)

Growth Hormone (GH), Active Protein (Cat# AAA161667)

Full Name

Active Growth Hormone (GH)

Gene Names

GH1; GH; GHN; GH-N; hGH-N; IGHD1B

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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Bioactivity (Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspase 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. Thus the activity of recombinant mouse caspase 3 can be measuered by calculating the concentration of the pNA released from the substrate. The reaction was performed in adding 50 ul 2×buffer (50mM HEPES,100mM NaCl,10mM DTT, 2mM EDTA, 10% glycerol) to 96 well plates, then add 50 ul various concentrations of caspase 3 (diluted by 1×buffer, 25mM HEPES, 50mM NaCl, 5mM DTT, 1mM EDTA, 5% glycerol) to each well, finally, add 5 ul 4mM Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37 degree C for 1h. p-Nitroaniline (pNA) Standard curve prepare by double dilute 200 uM pNA with 1×buffer and record the OD value at 405 nm. The specific activity of recombinant mouse caspase3 is >3000 pmol/min/ug.)

Caspase 3 (CASP3), Active Protein (Cat# AAA161756)

Full Name

Active Caspase 3 (CASP3)

Gene Names

Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human tonsil tissue using CD69 Rabbit PolymAb® (AAA28586, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

CD69, Monoclonal Antibody (Cat# AAA28586)

Full Name

CD69 Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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Rab8, Polyclonal Antibody (Cat# AAA13866)

Full Name

Rab8 Pan Polyclonal Antibody

Gene Names

Rab8; AAF49101; CG8287; Dm Rab8; dm-Rab8; DmelCG8287; DmRab8; DRab8; DRAB8; l(3)76BDu; Rab-r8; rab8

Reactivity

Reacts against human, rat, mouse, canine and monkey proteins.

Applications

Immunofluorescence, Western Blot

Pricing

MRPL12, Monoclonal Antibody (Cat# AAA24279)

Full Name

MRPL12 (Mitochondrial Ribosomal Protein L12, 39S Ribosomal Protein L12, Mitochondrial, 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML12) (AP)

Gene Names

MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Pepsinogen A (PGA), Monoclonal Antibody (Cat# AAA151501)

Full Name

Monoclonal Antibody to Pepsinogen A (PGA)

Gene Names

PGA5; Pg5

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

JUNB, Polyclonal Antibody (Cat# AAA19249)

Full Name

Anti-JUNB Antibody

Gene Names

JUNB; AP-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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Bioactivity (Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant rat antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 ug/ml in the assay buffer and 10 ul different concentrations of recombinant rat antithrombin III (MW: 52.6 KD) was incubated with 10 ul diluted thrombin at 37 degree C for 30 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat antithrombin III significantly decreased thrombin activity. The inhibition IC50 was )

Antithrombin (AT), Active Protein (Cat# AAA161887)

Full Name

Active Antithrombin (AT)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using ENTPD5 Rabbit mAb (AAA28519) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

ENTPD5, Monoclonal Antibody (Cat# AAA28519)

Full Name

ENTPD5 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ATF2 antibody (AAA11634).Overlay histogram showing HepG2 cells stained with AAA11634 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (AAA11634,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA11634)

Full Name

Anti-ATF2 Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

Low Density Lipoprotein, Native Protein (Cat# AAA10845)

Full Name

Human Low Density Lipoprotein (LDL)

Pricing

Heme oxygenase1, Recombinant Protein (Cat# AAA11763)

Full Name

Heme oxygenase1,1-266aa, Human, His tag, E Coli

Gene Names

HMOX1; HO-1; HSP32; HMOX1D; bK286B10

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

PTBP1, Monoclonal Antibody (Cat# AAA24331)

Full Name

PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (AP)

Gene Names

PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Sequence

Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150976)

Full Name

Active Angiotensin I Converting Enzyme 2 (ACE2)

Gene Names

ACE2; ACEH

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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Bioactivity (Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant chicken RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >50 uM.)

Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161804)

Full Name

Active Retinol Binding Protein 4 (RBP4)

Gene Names

RBP4A; RBP; RBP4

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

PEAK1, Recombinant Protein (Cat# AAA14247)

Full Name

PEAK1, Unactive recombinant protein

Gene Names

PEAK1; SGK269

Applications

Kinase Assay, Western Blot

Purity

>95%

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of Gpnmb using anti-Gpnmb antibody.)

GPNMB/Gpnmb, Polyclonal Antibody (Cat# AAA19201)

Full Name

Anti-GPNMB/Gpnmb Antibody

Gene Names

Gpnmb; ipd; Dchil; DC-HIL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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10000 results for "Recombinant Proteins" - showing 1900-1950

Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161902)

Ebola Virus NP, Recombinant Protein (Cat# AAA24009)

SAMD9L, Polyclonal Antibody (Cat# AAA19973)

DnaK, Monoclonal Antibody (Cat# AAA14655)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24421)

Cathepsin D, Polyclonal Antibody (Cat# AAA13859)

Calnexin, Monoclonal Antibody (Cat# AAA23894)

COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA27038)

ICAM-1/CD54, Monoclonal Antibody (Cat# AAA28578)

FRS2, Monoclonal Antibody (Cat# AAA28559)

FAM40B/STRIP2, Polyclonal Antibody (Cat# AAA19969)

HIV-1 gp140, Recombinant Protein (Cat# AAA13754)

FGF2, Polyclonal Antibody (Cat# AAA11513)

CD66a/CEACAM1, Monoclonal Antibody (Cat# AAA28579)

SSX1, Monoclonal Antibody (Cat# AAA106681)

SMAD4, Monoclonal Antibody (Cat# AAA24363)

HIV-1 tat, Recombinant Protein (Cat# AAA14180)

GLDC, Monoclonal Antibody (Cat# AAA28583)

COVID 19 Nucleocapsid (NP) N fragment protein (NX) Coronavirus, Recombinant Protein (Cat# AAA27950)

TOM20, Monoclonal Antibody (Cat# AAA28304)

CD163, Recombinant Protein (Cat# AAA10873)

S100A9, Polyclonal Antibody (Cat# AAA11645)

Aprotinin (AP), Active Protein (Cat# AAA161813)

Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)

Ki67, Monoclonal Antibody (Cat# AAA28581)

Hemoglobin A1c, Native Protein (Cat# AAA10832)

Fascin, Recombinant Protein (Cat# AAA10880)

HA-Tag, Monoclonal Antibody (Cat# AAA27030)

Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161776)

HA (H9N2)A/Hong Kong/1073/99), Recombinant Protein (Cat# AAA13761)

Hirudin, Active Protein (Cat# AAA10836)

VPS35, Monoclonal Antibody (Cat# AAA27700)

DFNA5, Polyclonal Antibody (Cat# AAA28161)

Growth Hormone (GH), Active Protein (Cat# AAA161667)

Caspase 3 (CASP3), Active Protein (Cat# AAA161756)

CD69, Monoclonal Antibody (Cat# AAA28586)

Rab8, Polyclonal Antibody (Cat# AAA13866)

MRPL12, Monoclonal Antibody (Cat# AAA24279)

Pepsinogen A (PGA), Monoclonal Antibody (Cat# AAA151501)

JUNB, Polyclonal Antibody (Cat# AAA19249)

Antithrombin (AT), Active Protein (Cat# AAA161887)

ENTPD5, Monoclonal Antibody (Cat# AAA28519)

Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA11634)

Low Density Lipoprotein, Native Protein (Cat# AAA10845)

Heme oxygenase1, Recombinant Protein (Cat# AAA11763)

PTBP1, Monoclonal Antibody (Cat# AAA24331)

Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150976)

Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161804)

PEAK1, Recombinant Protein (Cat# AAA14247)

GPNMB/Gpnmb, Polyclonal Antibody (Cat# AAA19201)

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