Search results for "Recombinant Proteins"

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (AAA28550) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

Fatty Acid Synthase (FASN), Monoclonal Antibody (Cat# AAA28550)

Full Name

[KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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FSP1/S100A4, Monoclonal Antibody (Cat# AAA28488)

Full Name

FSP1/S100A4 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Pea3 / ETV4 Rabbit pAb (AAA28094) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining)

ETV4, Polyclonal Antibody (Cat# AAA28094)

Full Name

ETV4 Polyclonal Antibody

Gene Names

ETV4; E1AF; PEA3; E1A-F; PEAS3

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

RPL36A, Monoclonal Antibody (Cat# AAA24342)

Full Name

RPL36A (60S Ribosomal Protein L36a, 60S Ribosomal Protein L44, Cell Migration-inducing Gene 6 Protein, Cell Growth-inhibiting Gene 15 Protein, RPL44, GIG15, MIG6, MGC72020) (AP)

Gene Names

RPL36A; L36A; L44L; MIG6; RPL44

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA215322)

Full Name

E-Cadherin/CD324 (Intercellular Junction Marker)

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27690)

Full Name

VPS35 Antibody, Clone 8A3: RPE

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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Application Data (In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgM sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the p-value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera.)

lipl32, Recombinant Protein (Cat# AAA14238)

Full Name

lipl32 recombinant

Applications

ELISA

Pricing

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S100B, Monoclonal Antibody (Cat# AAA28487)

Full Name

S100B Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Figure 17. IF analysis of HADHA using anti-HADHA antibody (AAA19797).HADHA was detected in an immunocytochemical section of MG63 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 15. IHC analysis of HADHA using anti-HADHA antibody (AAA19797).HADHA was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat kidney tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse kidney tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human HELA whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa.Lane 1: human 293T whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa.)

HADHA, Polyclonal Antibody (Cat# AAA19797)

Full Name

Anti-HADHA Antibody Picoband

Gene Names

HADHA; GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

DNAJB6, Monoclonal Antibody (Cat# AAA24192)

Full Name

DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (AP)

Gene Names

DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (AAA11633).Overlay histogram showing K562 cells stained with AAA11633 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASP3 Antibody (AAA11633,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Caspase-3, Polyclonal Antibody (Cat# AAA11633)

Full Name

Anti-Caspase-3 Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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Cation-independent M6PR (IGF2R), Monoclonal Antibody (Cat# AAA28549)

Full Name

Cation-independent M6PR (IGF2R) Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 21. Flow Cytometry analysis of RH35 cells using anti-RAB7/RAB7A antibody (AAA19775).Overlay histogram showing RH35 cells stained with AAA19775 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7/RAB7A Antibody (AAA19775, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 18. IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (AAA19775).RAB7/RAB7A was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB7/RAB7A Antibody (AAA19775) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U-87MG whole cell lysates,Lane 3: human HEL whole cell lysates.Lane 4: rat L6 whole cell lysates.Lane 5: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7/RAB7A antigen affinity purified polyclonal antibody (#AAA19775) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB7/RAB7A at approximately 23 kDa. The expected band size for RAB7/RAB7A is at 23 kDa.)

RAB7/RAB7A, Polyclonal Antibody (Cat# AAA19775)

Full Name

Anti-RAB7/RAB7A Antibody Picoband

Gene Names

RAB7A; RAB7; PRO2706

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

Urinary Trypsin Inhibitor, Active Protein (Cat# AAA10834)

Full Name

Human Urinary Trypsin Inhibitor (UTI)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cell using MMP16 antibody (AAA28228) at dilution of 1:100. Blue: DAPI for nuclear staining)

MMP16, Polyclonal Antibody (Cat# AAA28228)

Full Name

MMP16 Polyclonal Antibody

Gene Names

MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27703)

Full Name

VPS35 Antibody, Clone 11H10: ATTO 594

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27671)

Full Name

VPS35 Antibody, Clone 7E4: PerCP

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (AAA19348).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (AAA19348) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

Carbonic Anhydrase I/CA1, Monoclonal Antibody (Cat# AAA19348)

Full Name

Anti-Carbonic Anhydrase I/CA1 Antibody (monoclonal, 2B5)

Gene Names

CA1; CAB; CA-I; Car1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

KCNQ2, Polyclonal Antibody (Cat# AAA19238)

Full Name

Anti-KCNQ2 Antibody

Gene Names

KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

CD95, Recombinant Protein (Cat# AAA10856)

Full Name

Recombinant Human CD95, His Tag

Gene Names

FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6

Purity

Greater than 95% as determined by Analysis by SDS-PAGE.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TAS1R3 antibody (AAA28219) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.)

TAS1R3, Polyclonal Antibody (Cat# AAA28219)

Full Name

TAS1R3 Polyclonal Antibody

Gene Names

TAS1R3; T1R3

Applications

Western Blot

Purity

Affinity purification

Pricing

PDIA3, Recombinant Protein (Cat# AAA11788)

Full Name

PDIA3, 25-505aa, Human, His tag, E Coli

Gene Names

PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27687)

Full Name

VPS35 Antibody, Clone 8A3: FITC

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

DNAJC10, Monoclonal Antibody (Cat# AAA24193)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (AP)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat spleen using CD79a Rabbit PolymAb® (AAA28555) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

CD79a, Monoclonal Antibody (Cat# AAA28555)

Full Name

CD79a Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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JAB1/CSN5/COPS5, Monoclonal Antibody (Cat# AAA28603)

Full Name

JAB1/CSN5/COPS5 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation

Purity

Affinity purification

Pricing

SFN, Recombinant Protein (Cat# AAA18906)

Full Name

Recombinant Human SFN, N-His

Gene Names

SFN; YWHAS

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

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DNMT1, Polyclonal Antibody (Cat# AAA28335)

Full Name

DNMT1 Rabbit pAb

Gene Names

DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27693)

Full Name

VPS35 Antibody, Clone 10A8: ATTO 488

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman IBA1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Ionized Calciumbinding Adapter Molecule 1 (IBA1), Monoclonal Antibody (Cat# AAA21206)

Full Name

Monoclonal Antibody to Ionized Calciumbinding Adapter Molecule 1 (IBA1)

Gene Names

AIF1; IBA1; IRT1; AIF-1; IRT-1

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

HSP10, Recombinant Protein (Cat# AAA11744)

Full Name

HSP10, 1-102aa, Human, E Coli

Gene Names

HSPE1; EPF; CPN10; GROES; HSP10

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

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DBI, Polyclonal Antibody (Cat# AAA28262)

Full Name

DBI Polyclonal Antibody

Gene Names

DBI; EP; ACBP; ACBD1; CCK-RP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded rat colon tissue using PP2A Catalytic ? Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

PPP2CA, Antibody (Cat# AAA28190)

Full Name

PPP2CA Polyclonal Antibody

Gene Names

PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry

Purity

Affinity purification

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27691)

Full Name

VPS35 Antibody, Clone 10A8

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27689)

Full Name

VPS35 Antibody, Clone 8A3: PerCP

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERp29 Rabbit mAb (AAA28530) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

ERp29, Monoclonal Antibody (Cat# AAA28530)

Full Name

ERp29 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of HEL cells using anti-PITHD1 antibody (AAA20003).Overlay histogram showing HEL cells stained with AAA20003 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITHD1 Antibody (AAA20003, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 13. IHC analysis of PITHD1 using anti-PITHD1 antibody (AAA20003).PITHD1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITHD1 Antibody (AAA20003) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: rat thymus tissue lysates,Lane 5: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITHD1 antigen affinity purified polyclonal antibody (#AAA20003) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PITHD1 at approximately 24 kDa. The expected band size for PITHD1 is at 24 kDa.)

PITHD1, Polyclonal Antibody (Cat# AAA20003)

Full Name

Anti-PITHD1 Antibody Picoband

Gene Names

PITHD1; HT014; TXNL1CL; C1orf128

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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CX43, Polyclonal Antibody (Cat# AAA13870)

Full Name

Connexin 43 Polyclonal Antibody

Gene Names

cx43; gja1; cx43a1; zfCx43.3

Reactivity

Reacts against human, rat, mouse, canine and monkey proteins

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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WB (Western Blot)

APPL/APPL1, Polyclonal Antibody (Cat# AAA19200)

Full Name

Anti-APPL/APPL1 Antibody

Gene Names

APPL1; APPL; MODY14; DIP13alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27678)

Full Name

VPS35 Antibody, Clone 5A9: FITC

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27681)

Full Name

VPS35 Antibody, Clone 5A9: RPE

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27664)

Full Name

VPS35 Antibody, Clone 7E4

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (IHC of mouse kidney using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)

CD31, Polyclonal Antibody (Cat# AAA13874)

Full Name

Anti-CD31

Reactivity

Human, Rat, Mouse, Monkey and Canine proteins.

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Epitope Affinity Purified

Pricing

GroEL, Recombinant Protein (Cat# AAA11737)

Full Name

GroEL, 1-548aa, E Coli, E Coli

Gene Names

groL; ECK4137; groEL; JW4103; mopA

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

HSP90B1 (GRP94), Recombinant Protein (Cat# AAA11758)

Full Name

HSP90B1 (GRP94), 22-803aa, Human, His tag, E Coli

Gene Names

HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m

Applications

SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

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WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in PC-12.)

HSPA1B, Monoclonal Antibody (Cat# AAA26112)

Full Name

HSPA1B (Heat Shock 70kD Protein 1B, FLJ54328, HSP70-1B, HSP70-2, HSPA1A) (APC)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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Bioactivity (Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Studies have shown that fibrinogen can enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse FGB and recombinant human ITGb3. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ITGb3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse FGB and recombinant human ITGb3 was shown in Figure 1, the EC50 for this effect is 0.016 ug/mL.)

Fibrinogen Beta Chain (FGB), Active Protein (Cat# AAA161834)

Full Name

Active Fibrinogen Beta Chain (FGB)

Gene Names

Fgb; 2510049G14Rik

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27670)

Full Name

VPS35 Antibody, Clone 7E4: HRP

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse kidney tissue using HOPX Rabbit PolymAb® (A15537-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

HOPX, Monoclonal Antibody (Cat# AAA28463)

Full Name

HOPX Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

CPSF3, Monoclonal Antibody (Cat# AAA24470)

Full Name

CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73kD Subunit, CPSF 73kD Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73) APC

Gene Names

CPSF3; CPSF73; CPSF-73

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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10000 results for "Recombinant Proteins" - showing 2000-2050

Fatty Acid Synthase (FASN), Monoclonal Antibody (Cat# AAA28550)

FSP1/S100A4, Monoclonal Antibody (Cat# AAA28488)

ETV4, Polyclonal Antibody (Cat# AAA28094)

RPL36A, Monoclonal Antibody (Cat# AAA24342)

E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA215322)

VPS35, Monoclonal Antibody (Cat# AAA27690)

lipl32, Recombinant Protein (Cat# AAA14238)

S100B, Monoclonal Antibody (Cat# AAA28487)

HADHA, Polyclonal Antibody (Cat# AAA19797)

DNAJB6, Monoclonal Antibody (Cat# AAA24192)

Caspase-3, Polyclonal Antibody (Cat# AAA11633)

Cation-independent M6PR (IGF2R), Monoclonal Antibody (Cat# AAA28549)

RAB7/RAB7A, Polyclonal Antibody (Cat# AAA19775)

Urinary Trypsin Inhibitor, Active Protein (Cat# AAA10834)

MMP16, Polyclonal Antibody (Cat# AAA28228)

VPS35, Monoclonal Antibody (Cat# AAA27703)

VPS35, Monoclonal Antibody (Cat# AAA27671)

Carbonic Anhydrase I/CA1, Monoclonal Antibody (Cat# AAA19348)

KCNQ2, Polyclonal Antibody (Cat# AAA19238)

CD95, Recombinant Protein (Cat# AAA10856)

TAS1R3, Polyclonal Antibody (Cat# AAA28219)

PDIA3, Recombinant Protein (Cat# AAA11788)

VPS35, Monoclonal Antibody (Cat# AAA27687)

DNAJC10, Monoclonal Antibody (Cat# AAA24193)

CD79a, Monoclonal Antibody (Cat# AAA28555)

JAB1/CSN5/COPS5, Monoclonal Antibody (Cat# AAA28603)

SFN, Recombinant Protein (Cat# AAA18906)

DNMT1, Polyclonal Antibody (Cat# AAA28335)

VPS35, Monoclonal Antibody (Cat# AAA27693)

Ionized Calciumbinding Adapter Molecule 1 (IBA1), Monoclonal Antibody (Cat# AAA21206)

HSP10, Recombinant Protein (Cat# AAA11744)

DBI, Polyclonal Antibody (Cat# AAA28262)

PPP2CA, Antibody (Cat# AAA28190)

VPS35, Monoclonal Antibody (Cat# AAA27691)

VPS35, Monoclonal Antibody (Cat# AAA27689)

ERp29, Monoclonal Antibody (Cat# AAA28530)

PITHD1, Polyclonal Antibody (Cat# AAA20003)

CX43, Polyclonal Antibody (Cat# AAA13870)

APPL/APPL1, Polyclonal Antibody (Cat# AAA19200)

VPS35, Monoclonal Antibody (Cat# AAA27678)

VPS35, Monoclonal Antibody (Cat# AAA27681)

VPS35, Monoclonal Antibody (Cat# AAA27664)

CD31, Polyclonal Antibody (Cat# AAA13874)

GroEL, Recombinant Protein (Cat# AAA11737)

HSP90B1 (GRP94), Recombinant Protein (Cat# AAA11758)

HSPA1B, Monoclonal Antibody (Cat# AAA26112)

Fibrinogen Beta Chain (FGB), Active Protein (Cat# AAA161834)

VPS35, Monoclonal Antibody (Cat# AAA27670)

HOPX, Monoclonal Antibody (Cat# AAA28463)

CPSF3, Monoclonal Antibody (Cat# AAA24470)

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