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8299 results for "Phosphorylation" - showing 2550-2600
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody.)
MAP2K3, Polyclonal Antibody (Cat# AAA243361)
Full Name
Phospho-MAP2K3 (Ser189) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using FAK(phospho-Tyr576/Tyr577) Antibody.)
FAK, Polyclonal Antibody (Cat# AAA306699)
Full Name
FAK (phospho-Tyr576/Tyr577) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
WB (Western Blot)
(Western blot analysis of extract from MDA-MB-435 cells untreated or treated with UV, using MKK3 (Ab-189) antibody and MKK3 (phospho-Ser189) antibody.)
MKK3, Antibody (Cat# AAA110947)
Full Name
MKK3 (Phospho-Ser189) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(Western blot analysis of extract from HeLa cells, using ATF-2 (phospho-Thr71 or 53) antibody.)
ATF2, Antibody (Cat# AAA111134)
Full Name
ATF2 (Phospho-Thr71 or 53) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody. Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Phospho-Thr222) antibody.)
MAP2K3, Antibody (Cat# AAA110538)
Full Name
MAP2K3 (Phospho-Thr222) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(Western Blot analysis of extracts of 293T cells, using Phospho-FGFR1-Y653 pAb at 1:1000 dilution 293T cells were treated by EGF (25ug/ml) at 37 degree C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/Proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic KitExposure time: 30 seconds)
FGF Receptor 1-Y653, Antibody (Cat# AAA36646)
Full Name
Phospho-FGF Receptor 1-Y653 Polyclonal Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody.)
MARCKS, Polyclonal Antibody (Cat# AAA302858)
Full Name
MARCKS (phospho-Ser170) Antibody
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK alpha (Phospho-Thr23) Antibody.)
IKK alpha, Polyclonal Antibody (Cat# AAA307661)
Full Name
IKK alpha (Phospho-Thr23) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody. EPHA3/4/5 (Phospho-Tyr779/833) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
EPHA3/4/5, Antibody (Cat# AAA110046)
Full Name
EPHA3/4/5 (Phospho-Tyr779/833) Antibody
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left)or the same antibody preincubated with blocking peptide (right).)
WWOX, Polyclonal Antibody (Cat# AAA243217)
Full Name
Phospho-WWOX (Tyr33) Antibody
Gene Names
WWOX; FOR; WOX1; FRA16D; HHCMA56; PRO0128; SDR41C1; D16S432E
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
WB (Western Blot)
(Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKalpha1 (Phospho-Ser487) Antibody.)
AMPKalpha1, Polyclonal Antibody (Cat# AAA305016)
Full Name
AMPKalpha1 (Phospho-Ser487)Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) antibody.)
hnRPD, Antibody (Cat# AAA110513)
Full Name
hnRPD (Phospho-Ser83) Antibody
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKR (Phospho-Thr258) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using PKR (Phospho-Thr258) antibody.)
PKR, Antibody (Cat# AAA109750)
Full Name
PKR (Phospho-Thr258) Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
SDS-PAGE
B-Lymphocyte Chemoattractant 1 (BLC1), Recombinant Protein (Cat# AAA134584)
Full Name
Recombinant B-Lymphocyte Chemoattractant 1 (BLC1)
Gene Names
CXCL13; BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 97%
Pricing
SDS-PAGE
Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12), Recombinant Protein (Cat# AAA134699)
Full Name
Recombinant Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)
Gene Names
Siglece; Cd170; Siglec5; Siglec9; Siglec12; Siglecl1; mSiglec-E
Applications
Western Blot
Purity
> 95%
Pricing
DB (Dot Blot)
(Dot blot analysis of mouse TSC2 Antibody (Phospho S1343) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Phospho-mouse TSC2 (S1343), Polyclonal Antibody (Cat# AAA288358)
Full Name
Phospho-mouse TSC2 (S1343) Antibody
Reactivity
Mouse (Predicted Reactivity: Rat)
Applications
Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
SDS-PAGE
Trefoil Factor 3, Intestinal (TFF3), Recombinant Protein (Cat# AAA134277)
Full Name
Recombinant Trefoil Factor 3, Intestinal (TFF3)
Gene Names
TFF3; ITF
Reactivity
Canine (Dog)
Applications
CoImmunoprecipitation, Immunoprecipitation, Western Blot
Purity
>98%
Pricing
Application Data
Peptidase Inhibitor 16 (PI16), Recombinant Protein (Cat# AAA134061)
Full Name
Recombinant Peptidase Inhibitor 16 (PI16)
Gene Names
PI16; PSPBP; CRISP9; MSMBBP
Applications
Western Blot
Purity
> 97%
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKAR2A protein using PRKAR2A antibody)
PRKAR2A, Monoclonal Antibody (Cat# AAA108066)
Full Name
PRKAR2A antibody
Gene Names
PRKAR2A; PKR2; PRKAR2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
PRKAR2A antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extract from 293 cells treated or untreated with UV, using SEK1/MKK4 (phospho-Ser80) antibody.)
SEK1/MKK4, Antibody (Cat# AAA111159)
Full Name
SEK1/MKK4 (Phospho-Ser80) Antibody
Gene Names
MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(WB Suggested Anti-PINK1 AntibodyTitration: 1.0 ug/mlPositive Control: RPMI-8226 Whole Cell)
PINK1, Polyclonal Antibody (Cat# AAA201178)
Full Name
PINK1 antibody - N-terminal region
Gene Names
PINK1; BRPK; PARK6
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)
MAPK3, Polyclonal Antibody (Cat# AAA243302)
Full Name
Phospho-MAPK3 (Thr202) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
Gene Sequence
Kininogen 1, Recombinant Protein (Cat# AAA145918)
Full Name
Recombinant Kininogen 1 (KNG1)
Gene Names
KNG1; BK; BDK; KNG
Applications
Western Blot
Purity
> 90%
Pricing
WB (Western Blot)
(Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.)
ULK1, Polyclonal Antibody (Cat# AAA104185)
Full Name
ULK1 Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human; Mouse; Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Peptide Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(AAA321386 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SGK3, Polyclonal Antibody (Cat# AAA321386)
Full Name
Phospho-SGK3 (Thr320) Antibody
Gene Names
SGK3; CISK; SGK2; SGKL
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-AMPK beta1(Ser181) expression in various lysates)
AMPK beta1, Polyclonal Antibody (Cat# AAA321782)
Full Name
Phospho-AMPK beta1(Ser181) Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
GABAA Receptor, b3 (Ser408/409), Polyclonal Antibody (Cat# AAA72812)
Full Name
Anti-Phospho-Ser408/409 GABAA Receptor, beta3 subunit
Reactivity
Rat
Applications
Western Blot
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
Pricing
IHC (Immunohistochemisry)
(AAA321479 at 1/100 staining human Brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IR, Polyclonal Antibody (Cat# AAA321479)
Full Name
Phospho-IR (Tyr1355) Antibody
Gene Names
INSR; HHF5; CD220
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321578 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
PI3-kinase p85 alpha/gamma, Polyclonal Antibody (Cat# AAA321578)
Full Name
Phospho-PI3-kinase p85 alpha/gamma (Tyr467/199) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of CaMKII expression in (1)Mouse brain tissue lysate ;(2)Rat brain tissue lysate (AAA47138).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMK2B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAMK2B)
CaMKII, Monoclonal Antibody (Cat# AAA47138)
Full Name
Anti-CaMKII Rabbit Monoclonal Antibody
Gene Names
CAMK2B; CAM2; CAMK2; CAMKB; MRD54
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Affinity-chromatography
Pricing
IHC (Immunohistochemistry)
(AAA321643 at 1/100 staining human Skin cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
VASP, Polyclonal Antibody (Cat# AAA321643)
Full Name
Phospho-VASP (Ser157) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-p38 MAPK (Tyr182) expression in various lysates)
p38 MAPK, Polyclonal Antibody (Cat# AAA321743)
Full Name
Phospho-p38 MAPK (Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321486 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Progesterone Receptor, Polyclonal Antibody (Cat# AAA321486)
Full Name
Phospho-Progesterone Receptor (Ser294) Antibody
Gene Names
PGR; PR; NR3C3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Phospho-Catenin-beta (Ser37) Antibody.)
Catenin-beta, Polyclonal Antibody (Cat# AAA321593)
Full Name
Phospho-Catenin-beta (Ser37) Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PKD1/2/3/PKC mu (Ser738+Ser742) expression in various lysates)
PKD1/2/3/PKC mu, Polyclonal Antibody (Cat# AAA321734)
Full Name
Phospho-PKD1/2/3/PKC mu (Ser738+Ser742) Antibody
Gene Names
PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321748 staining Hela cells(20min of 30uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321748) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
CaMK4, Polyclonal Antibody (Cat# AAA321748)
Full Name
Phospho-CaMK4 (Thr196/200) Antibody
Gene Names
CAMK4; caMK; CaMK IV; CaMK-GR
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA321582 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Shc, Polyclonal Antibody (Cat# AAA321582)
Full Name
Phospho-Shc (Tyr427) Antibody
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-MUC1-T1224 Antibody (AAA287489) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Phospho-MUC1 (T1224), Polyclonal Antibody (Cat# AAA287489)
Full Name
Phospho-MUC1 (T1224) Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
Immunofluorescence, Dot Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with MERTK Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MERTK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MERTK immunoreactivity is localized to Vesicles significantly.)
MERTK, Polyclonal Antibody (Cat# AAA283689)
Full Name
MERTK Antibody
Gene Names
MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
DB (Dot Blot)
(DotBlotanalysisofHuman,Mouse,RatTau2N4RP301S(phospho&non-phospho)showingdetectionofTauproteinusingMouseAnti-TauMonoclonalAntibody,Clone15B7 .Lane1:hTau2N4RP301SMonomer(0.5ug).Lane2:hTau2N4RP301SMonomer(1.0ug).Lane3:RecombinanthTau-441(2N4R)P301SMonomer,Baculovirus/Sf9Expressed(phosphorylated)(0.5ug).Lane4:RecombinanthTau-441(2N4R)P301SMonomer,Baculovirus/Sf9Expressed(phosphorylated)(1.0ug).Load:0.5ug,1.0ug.Block:5%BSAinTBST.PrimaryAntibody:MouseAnti-TauMonoclonalAntibody at1:4forOvernight,4C,withshaking.SecondaryAntibody:Goatanti-mouseIgG:HRPat1:4000for1houratRTwithshaking.ColorDevelopment:ChemiluminescentforHRP(Moss)for5mininRT.DotBlotperformedwithsupernatants.)
Tau (pThr217) IgG1 Kappa, Monoclonal Antibody (Cat# AAA254066)
Full Name
Mouse Anti-Human Tau (pThr217) Monoclonal IgG1 Kappa
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Dot Blot, Western Blot
Purity
Protein G Purified
Pricing
WB (Western Blot)
(Western blot analysis of Amyloid beta A4 expression in mouse brain lysate (AAA46868).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APP)
Amyloid beta A4, Monoclonal Antibody (Cat# AAA46868)
Full Name
Anti-Amyloid beta A4 Rabbit Monoclonal Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-DNA Ligase IV/LIG4 antibody (AAA127077).Overlay histogram showing U937 cells stained with AAA127077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNA Ligase IV/LIG4 Antibody (AAA127077, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
DNA Ligase IV/LIG4, Polyclonal Antibody (Cat# AAA127077)
Full Name
Anti-DNA Ligase IV/LIG4 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies (for phosphorylation at Y319), (for phosphorylation at Y493), and AAA211720 (for phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, antibody (for total ZAP70) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
ZAP70, Polyclonal Antibody (Cat# AAA211720)
Full Name
Rabbit anti-Phospho ZAP70 (Y493/Y494) Antibody, Affinity Purified
Gene Names
ZAP70; SRK; STD; TZK; STCD; ZAP-70
Reactivity
Human
Applications
Immunoprecipitation
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.)
Acetylated Lysine, Monoclonal Antibody (Cat# AAA103346)
Full Name
Acetylated Lysine Antibody: ATTO 594
Reactivity
Bovine, Avian
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
WB (Western Blot)
(Western Blot analysis of NIH-3T3 cells using p73 Polyclonal Antibody)
p73, Polyclonal Antibody (Cat# AAA307865)
Full Name
p73 Polyclonal Antibody
Gene Names
TP73; P73
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western Blot analysis of RAT-MUSCLE cells using DARPP-32 Polyclonal Antibody)
PPP1R1B, Polyclonal Antibody (Cat# AAA236088)
Full Name
PPP1R1B Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(WB Suggested Anti-NF2 AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Brain)
NF2, Polyclonal Antibody (Cat# AAA201166)
Full Name
NF2 antibody - C-terminal region
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat heart tissue with Phospho-eIF2? (Ser51) Polyclonal Antibody at dilution of 1:200)
eIF2alpha, Polyclonal Antibody (Cat# AAA171881)
Full Name
Phospho-eIF2alpha (S51) Polyclonal Antibody
Gene Names
Eif2s1; 35kDa; Eif2a; eIF2alpha; 0910001O23Rik; 2410026C18Rik
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(Western Blot analysis of various cells with Phospho-Cot (Thr290) Polyclonal Antibody)
Cot, Polyclonal Antibody (Cat# AAA171783)
Full Name
Phospho-Cot (T290) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse lung tissue using c-Src Polyclonal Antibody at dilution of 1:200.)
c-Src, Polyclonal Antibody (Cat# AAA172415)
Full Name
c-Src Polyclonal Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing