Standard Curve (Sample)
General Antithrombin III total antigen assay ELISA Kit | SERPINC1 elisa kit
Human Antithrombin III total antigen assay ELISA Kit
Gene Names
SERPINC1; AT3; AT3D; ATIII; THPH7
Reactivity
General
Synonyms
Antithrombin III total antigen assay; N/A; Human Antithrombin III total antigen assay ELISA Kit; SERPINC1 elisa kit
Reactivity
General
Form/Format
Complete Kit
Sequence Length
464
Samples
Human plasma
Preparation and Storage
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SERPINC1 elisa kit
Intended Uses: This human Antithrombin III antigen assay is intended for the quantitative determination of total Antithrombin III antigen in human plasma.
Principle of the Assay: Human Antithrombin III will bind to the capture antibody coated on the microtiter plate. Free and complexed Antithrombin III will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Antithrombin III primary antibody binds to the captured protein Excess primary antibody is washed away and bound antibody, which is proportional to the total Antithrombin III present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified Antithrombin III and is measured along with the test samples. Color development is proportional to the concentration of Antithrombin III in the samples.
Background: Antithrombin III is a glycolsylated plasma serine protease inhibitor that forms a stoichiometric complex with coagulation cascade enzymes [1]. Antithrombin III inhibits alpha-Thrombin as well as Factor Xa, IXa, XIa and XIIa with heparin enhanced kinetics [2]. Type 1 Antithrombin deficiency is characterized by decreased plasma antigen levels of Antithrombin III [3].
Principle of the Assay: Human Antithrombin III will bind to the capture antibody coated on the microtiter plate. Free and complexed Antithrombin III will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Antithrombin III primary antibody binds to the captured protein Excess primary antibody is washed away and bound antibody, which is proportional to the total Antithrombin III present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified Antithrombin III and is measured along with the test samples. Color development is proportional to the concentration of Antithrombin III in the samples.
Background: Antithrombin III is a glycolsylated plasma serine protease inhibitor that forms a stoichiometric complex with coagulation cascade enzymes [1]. Antithrombin III inhibits alpha-Thrombin as well as Factor Xa, IXa, XIa and XIIa with heparin enhanced kinetics [2]. Type 1 Antithrombin deficiency is characterized by decreased plasma antigen levels of Antithrombin III [3].
Product Categories/Family for SERPINC1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,602 Da
NCBI Official Full Name
antithrombin-III
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol
SERPINC1
NCBI Official Synonym Symbols
AT3; AT3D; ATIII; THPH7
NCBI Protein Information
antithrombin-III; serpin C1; antithrombin III; serine-cysteine proteinase inhibitor clade C member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
UniProt Protein Name
Antithrombin-III
UniProt Gene Name
SERPINC1
UniProt Synonym Gene Names
AT3; ATIII
UniProt Entry Name
ANT3_HUMAN
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Product Notes
The General SERPINC1 serpinc1 (Catalog #AAA37772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37772 ELISA Kit recognizes General SERPINC1. It is sometimes possible for the material contained within the vial of "Antithrombin III total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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