SDS-PAGE
(Figure 1. Human TNFalpha Protein, His Tag on SDS-PAGE under reducing condition.)
TNFalpha recombinant protein
Recombinant human TNFalpha protein with C-terminal 6xHis tag
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Applications
Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
TNFalpha; N/A; Recombinant human TNFalpha protein with C-terminal 6xHis tag; Human TNFalpha Protein, His Tag; DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFalpha; TNFalpha recombinant protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-67% trehalose is added as protectants before lyophilization.
Sequence
TNFalpha(Val77-Leu233)
Applicable Applications for TNFalpha recombinant protein
WB (Western Blot)
Source
Human
Conjugation
C-6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice
Shipping: Blue Ice
SDS-PAGE
(Figure 1. Human TNFalpha Protein, His Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human TNFalpha Protein, His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human TNFalpha Protein, His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human TNFalpha Protein, His Tag on SDS-PAGE under reducing condition.)
Related Product Information for TNFalpha recombinant protein
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,644 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The TNFalpha tnf (Catalog #AAA47238) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNFalpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TNFalpha tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TNFalpha(V al77-Leu23 3). It is sometimes possible for the material contained within the vial of "TNFalpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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