Application Data
(Protease activity for extracted protein from mouse tissue while using different protease inhibitor cocktails)
Broad Spectrum Protease Inhibitor Cocktail Western Blot Related Reagent
Broad Spectrum Protease Inhibitor Cocktail
Synonyms
Broad Spectrum Protease Inhibitor Cocktail; N/A; Broad Spectrum Protease Inhibitor Cocktail western blot related reagent
Concentration
100-fold dilution in lysis buffer
10 u L of the Protease Inhibitor Cocktail solution is enough to inhibit degradation of proteins in 1mL (varies by lot)
10 u L of the Protease Inhibitor Cocktail solution is enough to inhibit degradation of proteins in 1mL (varies by lot)
Assay Principle
Broad Spectrum Protease Inhibitor Cocktail (100X) is a Western blot related ready-to-use concentrated stock solution reagent containing a blend of seven protease inhibitors that is to be added to cell lysis buffer to protect the integrity and functionality of native cellular proteins against degradation by multiple classes of endogenous proteases during protein extraction and sample preparation procedures. The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets.
Physical State
Colorless, transparent, liquid
Compatibility with Reagents
Fully compatible with cell lysis buffers and Broad Spectrum Phosphatase Inhibitor Cocktail
Compatibility with Assays
Not MS-compatible: contain AEBSF; Not compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis: contain EDTA
Reagent Type
Western Blotting related reagent; Inhibitors
Usage
Protect native cellular proteins from destructive degradation by endogenous proteases following cell lysis
Preserve native cellular proteins intact and functional
Screen extracts for proteolytic activity
Study proteolysis in the regulation of cellular processes
Preserve native cellular proteins intact and functional
Screen extracts for proteolytic activity
Study proteolysis in the regulation of cellular processes
Target Specificity
Serine proteases, cysteine proteases, aspartic acid proteases, metalloproteases, aminopeptidases
Target Samples
Cell lysis extracts
List of components
Broad Spectrum Protease Inhibitor Cocktail: Containing AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A stabilized in dimethylsulfoxide (DMSO), 1mL (100X), (MBS176729)
Broad Spectrum Protease Inhibitor Cocktail (EDTA solution): 1mL (100X) EDTA solution for optional metalloprotease inhibition ( )
Broad Spectrum Protease Inhibitor Cocktail (EDTA solution): 1mL (100X) EDTA solution for optional metalloprotease inhibition ( )
Preparation and Storage
Upon receipt store at -20°C. It is stable for one year. Product is shipped on ice.
Application Data
(Protease activity for extracted protein from mouse tissue while using different protease inhibitor cocktails)
Application Data
(Protease activity for extracted protein from mouse tissue while using different protease inhibitor cocktails)
Application Data
Application Data
Related Product Information for Broad Spectrum Protease Inhibitor Cocktail western blot related reagent
Broad Spectrum Protease Inhibitor Cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of intact and functional native proteins is to add inhibitors of these enzymes known to be present in the source material. This broad spectrum protease inhibitor cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.Protease inhibitors are critical reagents for the preservation of protein integrity during purification and analysis procedures by knocking out specific proteases to avoid peptide bond hydrolysis and subsequent protein destruction. They are biological or chemical compounds that function by reversibly or irreversibly binding to the protease. Most known proteases belong to one of four evolutionarily distinct enzyme families based on the functional groups involved in the peptide bondcleavage. No single chemical is effective for all types of proteases. A mixture or "cocktail" of several different inhibitor compounds is commonly used that consistently inhibit a multitude of protease classes to ensure that protein extracts do not degrade before analysisfor target proteins of interest. Not using protease inhibitors leads to the loss of a large number of valuable proteins in a lysate sample, adversely affecting downstream applications by biologically meaningless representation of protein activities and gaining false negative immunostaining results for targets of interest.
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of intact and functional native proteins is to add inhibitors of these enzymes known to be present in the source material. This broad spectrum protease inhibitor cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.Protease inhibitors are critical reagents for the preservation of protein integrity during purification and analysis procedures by knocking out specific proteases to avoid peptide bond hydrolysis and subsequent protein destruction. They are biological or chemical compounds that function by reversibly or irreversibly binding to the protease. Most known proteases belong to one of four evolutionarily distinct enzyme families based on the functional groups involved in the peptide bondcleavage. No single chemical is effective for all types of proteases. A mixture or "cocktail" of several different inhibitor compounds is commonly used that consistently inhibit a multitude of protease classes to ensure that protein extracts do not degrade before analysisfor target proteins of interest. Not using protease inhibitors leads to the loss of a large number of valuable proteins in a lysate sample, adversely affecting downstream applications by biologically meaningless representation of protein activities and gaining false negative immunostaining results for targets of interest.
Product Categories/Family for Broad Spectrum Protease Inhibitor Cocktail western blot related reagent
Similar Products
Product Notes
The Broad Spectrum Protease Inhibitor Cocktail (Catalog #AAA45621) is a Western Blot Related Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Broad Spectrum Protease Inhibitor Cocktail, Western Blot Related Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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