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IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)
Rabbit RUNX1 Polyclonal Antibody | anti-RUNX1 antibody
RUNX1 Polyclonal Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
RUNX1; Polyclonal Antibody; RUNX1 Polyclonal Antibody; AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2Ab; anti-RUNX1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
453
Applicable Applications for anti-RUNX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorecence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:100
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:100
Species
Human
Route
Synthetic Peptide
Immunogen
A synthetic peptide of human RUNX1
Calculated Molecular Weight
51kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat heart using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat heart using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of SH-SY5Y cells, using RUNX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
WB (Western Blot)
(Western blot analysis of extracts of SH-SY5Y cells, using RUNX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
Related Product Information for anti-RUNX1 antibody
AML1 (also known as Runx1, CBFA2, and PEBP2alphaB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFbeta and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
Product Categories/Family for anti-RUNX1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
453
NCBI Official Full Name
Runt-related transcription factor 1
NCBI Official Synonym Full Names
runt-related transcription factor 1
NCBI Official Symbol
RUNX1
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
NCBI Protein Information
runt-related transcription factor 1; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B; oncogene AML-1; AML1-EVI-1 fusion protein; acute myeloid leukemia 1 protein; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; core-binding factor, runt domain, alpha subunit 2; polyomavirus enhancer-binding protein 2 alpha B subunit
UniProt Protein Name
Runt-related transcription factor 1
UniProt Gene Name
RUNX1
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
UniProt Entry Name
RUNX1_HUMAN
Similar Products
Product Notes
The RUNX1 runx1 (Catalog #AAA10677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUNX1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RUNX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorecence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 ICC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:20 - 1:100. Researchers should empirically determine the suitability of the RUNX1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUNX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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