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ELISA
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
Mouse anti-Human IL3RA / CD123 Monoclonal Antibody | anti-IL3RA antibody
Mouse Monoclonal [clone 10B8E7] (IgG1) to Human IL3RA / CD123
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Ascites
Synonyms
IL3RA / CD123; Monoclonal Antibody; Mouse Monoclonal [clone 10B8E7] (IgG1) to Human IL3RA / CD123; Anti-IL3RA / CD123 Antibody (aa200-305; clone 10B8E7) IHC-plus; IL3RA; CD123 antigen; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL3R; IL3RY; CD123; HIL-3Ra; IL-3R-alpha; IL-3RA; IL3RAY; IL3RX; Human IL3RA; anti-IL3RA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10B8E7
Specificity
Human IL3RA / CD123
Purity/Purification
Ascites
Form/Format
Ascites, 0.03% sodium azide
Sequence Length
378
Applicable Applications for anti-IL3RA antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000), Flo, ICC (1:200 - 1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:2000)
Target Species
Human
Immunogen Description
Purified recombinant fragment of human IL3RA (AA: 200-305)
Immunogen Type
Recombinant protein
Immunogen
IL3RA / CD123 antibody was raised against purified recombinant fragment of human IL3RA (AA: 200-305)
Antigen Modification
aa200-305
Preparation and Storage
Short term +4 degree C; Long term -20 degree C
ELISA
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
ELISA
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
WB (Western Blot)
(Western blot of IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa))
WB (Western Blot)
(Western blot of IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa))
WB (Western Blot)
(Western blot of IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.)
WB (Western Blot)
(Western blot of IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.)
FCM (Flow Cytometry)
(Flow cytometry of Hela cells using IL3RA mouse mAb (green) and negative control (red).)
FCM (Flow Cytometry)
(Flow cytometry of Hela cells using IL3RA mouse mAb (green) and negative control (red).)
ICC (Immunocytochemistry)
(Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
ICC (Immunocytochemistry)
(Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohistochemistry)
(Anti-IL3RA / CD123 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-IL3RA / CD123 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-IL3RA / CD123 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohistochemistry)
(Anti-IL3RA / CD123 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Product Categories/Family for anti-IL3RA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,466 Da
NCBI Official Full Name
interleukin-3 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 3 receptor, alpha (low affinity)
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
NCBI Protein Information
interleukin-3 receptor subunit alpha; IL-3RA; IL-3R-alpha; CD123 antigen; IL-3R subunit alpha; IL-3 receptor subunit alpha; IL-3 receptor alpha SP2 isoform
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
UniProt Entry Name
IL3RA_HUMAN
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Product Notes
The IL3RA il3ra (Catalog #AAA12372) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 10B8E7] (IgG1) to Human IL3RA / CD123 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL3RA / CD123 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000), Flo, ICC (1:200 - 1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the IL3RA il3ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL3RA / CD123, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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