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IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)
Mouse anti-Human, Mouse MCM2 Monoclonal Antibody | anti-MCM2 antibody
Anti-MCM2
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
Ascitic Fluid
Synonyms
MCM2; Monoclonal Antibody; Anti-MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN; KIAA0030; MGC10606; anti-MCM2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B5-H4-A9
Purity/Purification
Ascitic Fluid
Form/Format
Liquid ; PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
904
Applicable Applications for anti-MCM2 antibody
Western Blot (WB), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-p), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC), Immunoprecipitation (IP)
Application Notes
WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Immunogen
Purified recombinant fragment of human MCM2 expressed in E Coli.
Research Field
Epigenetics and Nuclear Signaling
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) and negative control (red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) and negative control (red).)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody(green),and DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody(green),and DAPI (blue).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using MCM2 mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using MCM2 mouse mAb.)
WB (Western Blot)
(Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) and HEPG2 (6) cell lysate.)
WB (Western Blot)
(Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) and HEPG2 (6) cell lysate.)
WB (Western Blot)
(Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.)
WB (Western Blot)
(Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.)
Related Product Information for anti-MCM2 antibody
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 102 kDa
Observed MW: 125kDa
Observed MW: 125kDa
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 2
NCBI Official Symbol
MCM2
NCBI Official Synonym Symbols
BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
NCBI Protein Information
DNA replication licensing factor MCM2
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Gene Name
MCM2
UniProt Synonym Gene Names
BM28; CCNL1; CDCL1; KIAA0030
Similar Products
Product Notes
The MCM2 mcm2 (Catalog #AAA14110) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM2 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-p), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC), Immunoprecipitation (IP). WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100. Researchers should empirically determine the suitability of the MCM2 mcm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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