Human Complement Fragment 4d, C4d ELISA Kit | C4d elisa kit
Human Complement Fragment 4d, C4d ELISA Kit
Reactivity
Human
Synonyms
Complement Fragment 4d, C4d; N/A; Human Complement Fragment 4d, C4d ELISA Kit; Human Complement Fragment 4d; C4d ELISA Kit; Complement Fragment 4d; C4d; C4d elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human C4d. No significant cross-reactivity or interference between human C4d and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
< 0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for C4d elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4d has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4d present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4d is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4d bound in the initial step. The color development is stopped and the intensity of the color is measured.
