Monkey Creatine Kinase MB isoenzyme ELISA Kit | CKMB elisa kit

Monkey Creatine Kinase MB isoenzyme ELISA Kit

Cat. Num. AAA17018

• Gene Names: Ckm; Ckmm
• Reactivity: Monkey
• Synonyms: Creatine Kinase MB isoenzyme; Monkey Creatine Kinase MB isoenzyme ELISA Kit; CKMB elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of CKMB. No significant cross-reactivity or interference between CKMB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CKMB and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CKMB elisa kit

Principle of the Assay: CKMB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CKMB antibody and an CKMB-HRP conjugate. The assay sample and buffer are incubated together with CKMB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKMB concentration since CKMB from samples and CKMB-HRP conjugate compete for the anti-CKMB antibody binding site. Since the number of sites is limited, as more sites are occupied by CKMB from the sample, fewer sites are left to bind CKMB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKMB concentration in each sample is interpolated from this standard curve.

Intended Uses: This CKMB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CKMB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for CKMB elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6978661
• NCBI GeneID: 24265
• NCBI Accession #: NP_036662.1
• NCBI GenBank Nucleotide #: NM_012530.2
• Molecular Weight: 43,045 Da
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: Ckm
• NCBI Official Synonym Symbols: Ckmm
• NCBI Protein Information: creatine kinase M-type; M-CK; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• UniProt Gene Name: Ckm
• UniProt Synonym Gene Names: Ckmm
• UniProt Entry Name: KCRM_RAT