Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
Cart Login
product-image-AAA23787_WB7.jpg
WB (Western Blot)
>
<
product-image-AAA23787_WB6.jpg
product-image-AAA23787_IP5.jpg
product-image-AAA23787_IHC4.jpg
product-image-AAA23787_IHC3.jpg
product-image-AAA23787_ICC2.jpg
product-image-AAA23787_FCM.jpg

BRD4 recombinant antibody

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]

Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
BRD4, Recombinant Antibody; Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]; HUNK1; Protein HUNK1; HUNKI; chromosome-associated protein; CAP; bromodomain-containing protein 4; MCAP; Bromodomain-containing protein 4; bromodomain containing 4; BRD4 recombinant antibody
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BL-151-6F11
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for BRD4 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Immunocytochemistry-Immunofluorescence (ICC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IHC-IF: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC-IF: 1:25-1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 1312 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot)

(Detection of human BRD4 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (lot AAA23787-2). For blotting immunoprecipitated BRD4, AAA23787 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

IHC (Immunohistochemistry)

(Detection of human BRD4 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human BRD4 by immunocytochemistry. Sample: formaldehyde-fixed Hep-G2 cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

FCM (Flow Cytometry)

(Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for BRD4 recombinant antibody
Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.
Product Categories/Family for BRD4 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
155.8kDa
NCBI Official Full Name
bromodomain-containing protein 4 isoform long
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
NCBI Protein Information
bromodomain-containing protein 4
UniProt Protein Name
Bromodomain-containing protein 4
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN

Similar Products

product-image-AAA18386_SC.png

BRD4 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA23786_WB8.jpg

BRD4 Recombinant Antibody

Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation, Flow Cytometry, Functional Assay
product-image-AAA27362_SC.jpg

BRD4 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA23787_WB7.jpg

BRD4 Recombinant Antibody

Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
product-image-AAA16104_SC.jpg

MAG ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA16409_SC.jpg

MOG ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA18236_SC.png

MOG ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA15357_SC.png

myelin basic protein (MBP) antibody ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA18674_SDS_PAGE.jpg

PMP2 Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA17607_SC.jpg

beta2-GP1 Ab IgG ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA17456_SC.jpg

CD59 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA11509_IHC2.jpg

MBP Antibody

Host: Rabbit
Reactivity: Mouse, Rat
Apps: Western Blot, Immunohistochemistry, Immunofluorescence
product-image-AAA16405_SC.jpg

MBP ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA23143_SC.png

Plp1 ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA14968_SC.png

MBP ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA17604_SC.jpg

MBP ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA16366_SC.jpg

MBP ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA18731_SDS_PAGE.jpg

G Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA20934_WB.jpg

GP1Ba Antibody

Host: Rabbit
Reactivity: Mus musculus (Mouse)
Apps: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Product Notes

The BRD4 brd4 (Catalog #AAA23787) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Immunocytochemistry-Immunofluorescence (ICC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC-IF: 1:25-1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the BRD4 brd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.