53BP1 recombinant antibody

Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11]

Cat. Num. AAA23789

• Gene Names: TP53BP1; p202; 53BP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: 53BP1; Monoclonal Recombinant Antibody; Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11]; p53-binding protein 1; tumor suppressor p53-binding protein 1; tumor protein 53-binding protein; 1; TP53-binding protein 1; p53BP1; p202; TDRD30; Tumor suppressor p53-binding protein 1; tumor protein p53 binding protein 1; 53BP1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BL-250-1H11
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for 53BP1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000 (3-8% Tris-acetate gel recommended); IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 350 and 400
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 second.)

IP (Immunoprecipitation)

(Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used for IP at 20 ul per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody . For blotting immunoprecipitated 53BP1, AAA23789 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 second.)

IHC (Immunohistochemistry)

(Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for 53BP1 recombinant antibody

53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Product Categories/Family for 53BP1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5032189
• NCBI GeneID: 7158
• NCBI Accession #: NP_005648.1
• NCBI GenBank Nucleotide #: NM_005657.2
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4; F8VY86
• Molecular Weight: 213,989 Da
• NCBI Official Full Name: tumor suppressor p53-binding protein 1 isoform 3
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: p202; 53BP1
• NCBI Protein Information: tumor suppressor p53-binding protein 1; p53BP1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
• UniProt Protein Name: Tumor suppressor p53-binding protein 1
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: 53BP1; p53-binding protein 1; p53BP1
• UniProt Entry Name: TP53B_HUMAN