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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Rabbit Acetyl-Histone H4-K5/K8/K12/K16 Polyclonal Antibody | anti-H4-K5/K8/K12/K16 antibody

Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb

Gene Names
FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
Acetyl-Histone H4-K5/K8/K12/K16; Polyclonal Antibody; Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb; H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; HIST2H4; HIST2H4A; anti-H4-K5/K8/K12/K16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHADVGKE
Applicable Applications for anti-H4-K5/K8/K12/K16 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Positive Samples
NIH/3T3, C6
Immunogen
A synthetic acetylated peptide around K5/K8/K12/K16 of human Histone H4.
Cellular Location
extracellular exosome, extracellular region, nucleoplasm, nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot)

(Western blot analysis of extracts of C6 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of extracts of C6 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

WB (Western Blot)

(Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Related Product Information for anti-H4-K5/K8/K12/K16 antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Product Categories/Family for anti-H4-K5/K8/K12/K16 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
166,462 Da
NCBI Official Full Name
Fanconi anemia group D2 protein isoform b
NCBI Official Synonym Full Names
Fanconi anemia, complementation group D2
NCBI Official Symbol
FANCD2
NCBI Official Synonym Symbols
FA4; FAD; FACD; FAD2; FA-D2; FANCD
NCBI Protein Information
Fanconi anemia group D2 protein
UniProt Protein Name
Fanconi anemia group D2 protein
UniProt Gene Name
FANCD2
UniProt Synonym Gene Names
FACD; Protein FACD2
UniProt Entry Name
FACD2_HUMAN

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Product Notes

The H4-K5/K8/K12/K16 fancd2 (Catalog #AAA28423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-Histone H4-K5/K8/K12/K16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the H4-K5/K8/K12/K16 fancd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVSKRRLSKS EDKESLTEDA SKTRKQPLSK KTKKSHIANE VEENDSIFVK LLKISGIILK TGESQNQLAV DQIAFQKKLF QTLRRHPSYP KIIEEFVSGL ESYIEDEDSF RNCLLSCERL QDEEASMGAS YSKSLIKLLL GIDILQPAII KTLFEKLPEY FFENKNSDEI NIPRLIVSQL KWLDRVVDGK DLTTKIMQLI SIAPENLQHD IITSLPEILG DSQHADVGKE. It is sometimes possible for the material contained within the vial of "Acetyl-Histone H4-K5/K8/K12/K16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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