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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Stat-1alpha/beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
Rabbit anti-Human, Mouse Stat-1 alpha/beta Polyclonal Antibody | anti-STAT1 antibody
Stat-1 alpha/beta Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified
Synonyms
Stat-1 alpha/beta; Polyclonal Antibody; Stat-1 alpha/beta Antibody; CANDF7 antibody; DKFZp686B04100 antibody; ISGF 3 antibody; ISGF3 antibody; OTTHUMP00000163552 antibody; OTTHUMP00000165046 antibody; OTTHUMP00000165047 antibody; OTTHUMP00000205845 antibody; Signal transducer and activator of transcription 1 antibody; Signal transducer and activator of transcription 1; 91kDa antibody; Signal transducer and activator of transcription 1-alpha/beta antibody; Stat1 antibody; STAT1_HUMAN antibody; STAT91 antibody; Transcription factor ISGF-3 components p91/p84 antibody; anti-STAT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-STAT1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:200, ICC: 1:200
FC/FACS: 1:100-1:200
IHC: 1:200, ICC: 1:200
FC/FACS: 1:100-1:200
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Stat-1alpha/beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Stat-1alpha/beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Stat-1��/�� in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Stat-1��/�� in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Stat-1alpha/beta in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Stat-1alpha/beta in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Stat-1alpha/beta in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Stat-1alpha/beta in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1��/�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1��/�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal human kidney tissue using anti-Stat-1��/�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal human kidney tissue using anti-Stat-1��/�� antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1alpha/beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1alpha/beta antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Stat-1alpha/beta on different cell lysates using anti-Stat-1alpha/beta antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7)
WB (Western Blot)
(Western blot analysis of Stat-1alpha/beta on different cell lysates using anti-Stat-1alpha/beta antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7)
Related Product Information for anti-STAT1 antibody
STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to IFN-gamma stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element; in response to either IFN-alpha or IFN-beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon-Stimulated Response Element) promoter element. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.
Product Categories/Family for anti-STAT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,335 Da
NCBI Official Full Name
signal transducer and activator of transcription 1-alpha/beta isoform alpha
NCBI Official Synonym Full Names
signal transducer and activator of transcription 1, 91kDa
NCBI Official Symbol
STAT1
NCBI Official Synonym Symbols
CANDF7; ISGF-3; STAT91
NCBI Protein Information
signal transducer and activator of transcription 1-alpha/beta; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1
UniProt Protein Name
Signal transducer and activator of transcription 1-alpha/beta
UniProt Gene Name
STAT1
UniProt Entry Name
STAT1_HUMAN
Similar Products
Product Notes
The STAT1 stat1 (Catalog #AAA29957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Stat-1 alpha/beta Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Stat-1 alpha/beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:200, ICC: 1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the STAT1 stat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stat-1 alpha/beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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