WB (Western Blot)
(Western blot analysis of Hela, diluted at 1:1000.)
Mouse Collagen III (1D4) Monoclonal Antibody | anti-1D4 antibody
Collagen III (1D4) Mouse mAb
IF: 1:200
IHC: 1:50-300
WB (Western Blot)
(Western blot analysis of Hela, diluted at 1:1000.)
WB (Western Blot)
(Western blot analysis of Hela, diluted at 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse-liver tissue. 1, Collagen III Monoclonal Antibody (Q76) (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse-liver tissue. 1, Collagen III Monoclonal Antibody (Q76) (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Collagen III Monoclonal Antibody (Q76) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98 degree C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot)
(Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4 degree over night, secondary antibody was diluted at 1:10000, 37 degree 1hour. (Red) Actin beta Polyclonal Antibody was diluted at 1:5000 as loading control, 4 degree over night, secondary antibody was diluted at 1:10000, 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4 degree over night, secondary antibody was diluted at 1:10000, 37 degree 1hour. (Red) Actin beta Polyclonal Antibody was diluted at 1:5000 as loading control, 4 degree over night, secondary antibody was diluted at 1:10000, 37 degree 1hour.)
NCBI and Uniprot Product Information
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Product Notes
The 1D4 col3a1 (Catalog #AAA30540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Collagen III (1D4) Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen III (1D4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000 IF: 1:200 IHC: 1:50-300. Researchers should empirically determine the suitability of the 1D4 col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen III (1D4), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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