CTLA-4 Fc Recombinant Protein | CTLA-4 recombinant protein

Human CTLA-4 Fc

Cat. Num. AAA79322

• Gene Names: CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• Reactivity: Human
• Synonyms: CTLA-4 Fc; N/A; Human CTLA-4 Fc; Cytotoxic T-Lyphocyte-associated antigen 4; CD152; CTLA-4 recombinant protein

• Host: E. coli
• Reactivity: Human
• Form/Format: Lyophilized (10mM Sodium Phosphate, pH 7.5 + 25mM NaCl)!!Length (aa)||357
• Sequence: MHVAQPAVVL ASSRGIASFV CEYASPGKAT EVRVTVLRQA DSQVTE VCAA TYMMGNELTF LDDSICTGTS SGNQVNLTIQ GLRAMDTGLY I CKVELMYPP PYYLGIGNGT QIYVIDPEPC PDSDGGPKSC DKTHTCP PCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PE VKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKE YK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQV SLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSK L TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK
• Sequence Length: 223

• Endotoxin Level: < 0.1ng/ug of protein (<1EU/ug).
• Resconstitution: Centifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at 20°C to -80°C.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. Avoid repeated freeze and thaw cycles.

Related Product Information for CTLA-4 recombinant protein

CTLA-4 and CD28 are receptors of the immunoglobulin superfamily that are expressed, along with the transmembrane glycoproteins B7-1 and B7-2, by antigen-presenting cells, and with these ligands constitute crucial co-stimulatory pathways for T and B cell regulatory responses. It is through engagement with CD28 and CTLA-4 that the B7 family ligands B7-1 and B7-2 play principal roles in immunity by activating immune response and maintaining immune tolerance. Co-stimulatory signals generated by B7-1 and B7-2 interactions with CD28 serve to stimulate T cell activation and prevent anergy through the amplification of T cell receptor (TCR) signaling. In contrast, interactions of the ligands with CTLA-4 serves to maintain T cell homeostasis and self-tolerance through the disruption of stimulatory signaling from B7 isoform-bound CD28 complexes, and by inducing powerful inhibitory signals in T cells. CTLA-4, like B7-1, is only poorly expressed on resting dendritic cells; therefore, up-regulation of their interaction and resultant amplification and regulation of T cell activity at peripheral inflammation sites is considerably delayed upon immune activation. Conversely, B7-2 and CD28 are constitutively expressed by resting hematopoietic and T cells, respectively, and as a result are able to rapidly induce up-regulation upon immune activation, making them critical to the early co-stimulatory signaling of immune response. Unlike B7-1 and B7-2, the ligands PD-L1 (or B7-H1) and B7-H2, which also belong to the B7 family, have not been shown to influence immunity through interaction with CTLA-4. B7-H2 has been shown to have restricted interaction with CD28. The difference in expression of B7-1, B7-2 and B7-H2 may enable temporally and spatially-specific regulation of T cell response through non-competitive CD28 interaction. Recombinant Human CTLA-4 Fc is a glycosylated, disulfide-linked homodimer of 714 amino acid residues whose monomer consists of the 124-amino-acid length extracellular portion of CTLA-4 fused to the 231-amino-acid length Fc portion of human IgG1 by two glycines. The calculated molecular weight of Recombinant Human CTLA-4 Fc dimer is 78.7 kDa; however, due to glycosylation, the monomer and dimer migrate at apparent molecular weights of approximately 45 -50 kDa and 80 -90 kDa by SDS-PAGE analysis under reducing conditions.

Product Categories/Family for CTLA-4 recombinant protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 27735177
• NCBI GeneID: 1493
• UniProt Accession #: P16410; O95653; Q0PP65; Q52MC1; Q53TD5; Q5S005; Q8WXJ1; Q96P43; Q9UKN9; A0N1S0; E9PDH0
• Molecular Weight: Calculated MW: 78.7 kDa
• NCBI Official Full Name: Cytotoxic T-lymphocyte protein 4
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte associated protein 4
• NCBI Official Symbol: CTLA4
• NCBI Official Synonym Symbols: CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• UniProt Gene Name: CTLA4
• UniProt Synonym Gene Names: CD152; CTLA-4