Goat SOX2 Antibody | anti-SOX2 antibody

Goat Anti-SOX2 Antibody

Cat. Num. AAA61826

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SOX2; N/A; Goat Anti-SOX2 Antibody; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2; anti-SOX2 antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 317

• Applicable Applications for anti-SOX2 antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence YNMMETELKPPGPQQ-C, from the N Terminus of the protein sequence according to NP_003097.1.
• Peptide Sequence: YNMMETELKPPGPQQ
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

IHC (Immunohistochemistry)

(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)

IHC (Immunohistochemisry)

((8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

WB (Western Blot)

((0.3ug/ml) staining of MCF7 (A) HEK293 (B) and MCF7 nuclear (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

References

Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD.: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80. PMID: 15849174

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NP_003097.1
• UniProt Accession #: P48431
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY-box transcription factor 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN