ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight 594.)
Rabbit Sox2 Polyclonal Antibody | anti-Sox2 antibody
Sox2 (Thr-118), phospho-specific Antibody
Gene Names
Sox2; lcc; ysb; Sox-2
Reactivity
Human, Rat, Mouse, Chicken, Fish
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
Sox2, Antibody; Sox2 (Thr-118), phospho-specific Antibody; Sox2 (Thr-118), phospho-specific; Sox2 118T; anti-Sox2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Fish
Clonality
Polyclonal
Specificity
The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-118) peptide before affinity purification using phospho-Sox2 (Thr-118) peptide. This antibody antibody detects a 34kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells. This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Sox2 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-Sox2 (Thr-118) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-118 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Thr-126) and Sox3 (Thr-144), but low homology to other Sox family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight 594.)
WB (Western Blot)
(Western blot image of mouse F9 stem cells treated with with calyculin A (100nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).)
WB (Western Blot)
(Western blot image of mouse F9 stem cells treated with with calyculin A (100nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).)
Related Product Information for anti-Sox2 antibody
Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34kDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 2
NCBI Official Symbol
Sox2
NCBI Official Synonym Symbols
lcc; ysb; Sox-2
NCBI Protein Information
transcription factor SOX-2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
Sox2
UniProt Synonym Gene Names
Sox-2
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Product Notes
The Sox2 sox2 (Catalog #AAA71715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sox2 (Thr-118), phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken, Fish and may cross-react with other species as described in the data sheet. AAA Biotech's Sox2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Sox2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sox2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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