FCM/FACS (Flow Cytometry)
(SOX2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit SOX2 Polyclonal Antibody | anti-SOX2 antibody
SOX2 Antibody (Center)
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human; Predicted Reactivity: Zebrafish, Chicken, Mouse, Xenopus
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
SOX2, Antibody; SOX2 Antibody (Center); Transcription factor SOX-2; SOX2; anti-SOX2 antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Zebrafish, Chicken, Mouse, Xenopus
Clonality
Polyclonal
Isotype
Ig
Specificity
This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human SOX2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
317
Applicable Applications for anti-SOX2 antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot)
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Nucleus.
Antigen Region
89-119 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SOX2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SOX2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SOX2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SOX2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Anti-SOX2 Antibody (Center) at 1:4000 dilution + NCCIT whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-SOX2 Antibody (Center) at 1:4000 dilution + NCCIT whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SOX2 antibody
Description: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
Background: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Background: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Product Categories/Family for anti-SOX2 antibody
References
Remenyi, A., et al., Genes Dev. 17 (16):2048-2059 (2003). Wiebe, M.S., et al., J. Biol. Chem. 278 (20):17901-17911 (2003). Fantes, J., et al., Nat. Genet. 33 (4):461-463 (2003). Schepers, G.E., et al., Dev. Cell 3 (2):167-170 (2002). Kamachi, Y., et al., Trends Genet. 16 (4):182-187 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34310 Da
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY-box 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
Similar Products
Product Notes
The SOX2 sox2 (Catalog #AAA290681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOX2 Antibody (Center) reacts with Human; Predicted Reactivity: Zebrafish, Chicken, Mouse, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's SOX2 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.