IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic stainin)
Goat BRM Antibody | anti-BRM antibody
Goat Anti-BRM Antibody
Gene Names
SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
Reactivity
HumanExpected from sequence similarity: Human, Rat
Applications
Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BRM; N/A; Goat Anti-BRM Antibody; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 ; BAF190 ; BRM ; FLJ36757 ; MGC74511 ; SNF2 ; SNF2L2 ; SNF2LA ; SWI2 ; Sth1p ; hBRM ; hSNF2a; SNF2-alpha; SNF2-like 2 ; SNF2/SWI2-like protein 2 ; SWI/SNF-re; anti-BRM antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human, Rat
Expected from sequence similarity: Human, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
1590
Applicable Applications for anti-BRM antibody
IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-PSNSQLEIEGNSS, from the internal region of the protein sequence according to NP_003061.3; NP_620614.2.
Peptide Sequence
PSNSQLEIEGNSS
Epitope
Internal region
Important comments
This antibody is expected to recognise both reported isoforms (NP_003061.3; NP_620614.2)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic stainin)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic stainin)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic stainin)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic stainin)
References
Reisman DN, Sciarrotta J, Bouldin TW, Weissman BE, Funkhouser WK.: The expression of the SWI/SNF ATPase subunits BRG1 and BRM in normal human tissues. Appl Immunohistochem Mol Morphol. 2005 Mar;13(1):66-74. PMID: 15722796
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
SMARCA2
NCBI Official Synonym Symbols
BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
NCBI Protein Information
probable global transcription activator SNF2L2
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Gene Name
SMARCA2
UniProt Synonym Gene Names
BAF190B; hBRM
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Product Notes
The BRM smarca2 (Catalog #AAA61829) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-BRM Antibody reacts with Human Expected from sequence similarity: Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRM can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the BRM smarca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRM, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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