Application Data
(ADP-Glo assay for SMARCA2/BRM activity 100 uM ATP and 10 nM Salmon sperm DNA was incubated with different concentrations of SMARCA2/BRM protein in a 10 ul reaction system containing 20 mM HEPES (pH 7.5) ,10mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, then add 10 ul ADP-Glo Reagent at 25? for 60min.Then 20 ul Kinase. Detection Reagent incubates at 25? for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SMARCA2/BRM recombinant protein
Recombinant SMARCA2/BRM protein
Synonyms
SMARCA2/BRM; N/A; Recombinant SMARCA2/BRM protein; Snf2l2; brahma; SNF2alpha; BRM; SMARCA2/BRM recombinant protein
Host
Baculovirus
Form/Format
Recombinant SMARCA2/BRM protein in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant SMARCA2/BRM protein was expressed in a baculovirus expression system as the full length protein (accession number NP_003061.3) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 182.6kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(ADP-Glo assay for SMARCA2/BRM activity 100 uM ATP and 10 nM Salmon sperm DNA was incubated with different concentrations of SMARCA2/BRM protein in a 10 ul reaction system containing 20 mM HEPES (pH 7.5) ,10mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, then add 10 ul ADP-Glo Reagent at 25? for 60min.Then 20 ul Kinase. Detection Reagent incubates at 25? for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
Application Data
(ADP-Glo assay for SMARCA2/BRM activity 100 uM ATP and 10 nM Salmon sperm DNA was incubated with different concentrations of SMARCA2/BRM protein in a 10 ul reaction system containing 20 mM HEPES (pH 7.5) ,10mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, then add 10 ul ADP-Glo Reagent at 25? for 60min.Then 20 ul Kinase. Detection Reagent incubates at 25? for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE
(Recombinant SMARCA2/BRM protein 10% SDS-PAGE Coomassie staining MW: 182.6kDa Purity: >=95%)
SDS-PAGE
(Recombinant SMARCA2/BRM protein 10% SDS-PAGE Coomassie staining MW: 182.6kDa Purity: >=95%)
Related Product Information for SMARCA2/BRM recombinant protein
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is like the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes.SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.
Product Categories/Family for SMARCA2/BRM recombinant protein
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Product Notes
The SMARCA2/BRM (Catalog #AAA60418) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SMARCA2/BRM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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