Application Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
SMARCA2/BRM recombinant protein
Recombinant SMARCA2/BRM (1367-1511), GST-Tag
Gene Names
SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Synonyms
SMARCA2/BRM; N/A; Recombinant SMARCA2/BRM (1367-1511), GST-Tag; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; SMARCA2/BRM recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >95% pure by SDS-PAGE.
Form/Format
Recombinant SMARCA2/BRM (1367-1511), GST-Tag protein was expressed in E Coli and supplied in 25mM Tris-HCl pH8.0, 150mM NaCl and 10% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant SMARCA2/BRM (1367-1511), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Application Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Application Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Application Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
SDS-PAGE
(Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
Related Product Information for SMARCA2/BRM recombinant protein
Short Description: The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2/BRM (accession number NP_003061.3) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 44.2 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/14, H4K8, H4K12, H4K16 and H4K5/8/12/16. The recombinant protein is >=95% pure by SDS-PAGE. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.
Product Categories/Family for SMARCA2/BRM recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
SMARCA2
NCBI Official Synonym Symbols
BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
NCBI Protein Information
probable global transcription activator SNF2L2
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Gene Name
SMARCA2
UniProt Synonym Gene Names
BAF190B; hBRM
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Product Notes
The SMARCA2/BRM smarca2 (Catalog #AAA59710) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SMARCA2/BRM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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