WB (Western Blot)
(Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, KG-1, Daudi, THP-1, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
SMARCA2/BRM recombinant antibody
Rabbit anti-SMARCA2/BRM Recombinant Monoclonal Antibody [BLR301M]
Gene Names
SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
SMARCA2/BRM, Recombinant Antibody; Rabbit anti-SMARCA2/BRM Recombinant Monoclonal Antibody [BLR301M]; SNF2LA; SNF2; SNF2/SWI2-like protein 2; SNF2-alpha; SNF2L2; SWI2; Sth1p; protein brahma homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; BAF190; sucrose nonfermenting 2-like protein 2; ATP-dependent helicase SMARCA2; BAF190B; brahma homolog; BRG1-associated factor 190B; BRM; global transcription activator homologous sequence; hBRM; hSNF2a; NCBRS; probable global transcription activator SNF2L2; Probable global transcription activator SNF2L2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; SMARCA2/BRM recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR301M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for SMARCA2/BRM recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1325 and 1375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, KG-1, Daudi, THP-1, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, KG-1, Daudi, THP-1, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used for IP at 20 ul/mg lysate. SMARCA2/BRM was also immunoprecipitated by a second antibody against a different epitope of SMARCA2/BRM . For blotting immunoprecipitated SMARCA2/BRM, AAA213699 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699 lot 1) used for IP at 20 ul/mg lysate. SMARCA2/BRM was also immunoprecipitated by a second antibody against a different epitope of SMARCA2/BRM . For blotting immunoprecipitated SMARCA2/BRM, AAA213699 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of human SMARCA2/BRM (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human SMARCA2/BRM (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA213699) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for SMARCA2/BRM recombinant antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA2/Brm is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex).
Product Categories/Family for SMARCA2/BRM recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
SMARCA2
NCBI Official Synonym Symbols
BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
NCBI Protein Information
probable global transcription activator SNF2L2
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Gene Name
SMARCA2
UniProt Synonym Gene Names
BAF190B; hBRM
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Product Notes
The SMARCA2/BRM smarca2 (Catalog #AAA213699) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMARCA2/BRM Recombinant Monoclonal Antibody [BLR301M] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA2/BRM can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMARCA2/BRM smarca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA2/BRM, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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