IF (Immunofluorescence)
(SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 ug/ml dilution.)
Rat anti-Human, Mouse SMARCA2/BRM Monoclonal Antibody | anti-SMARCA2/BRM antibody
SMARCA2/BRM antibody (mAb)(Clone 1H7A10)
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
SMARCA2/BRM, Antibody; SMARCA2/BRM antibody (mAb)(Clone 1H7A10); Snf2l2; brahma; SNF2alpha; anti-SMARCA2/BRM antibody
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1H7A10
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-SMARCA2/BRM antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
Immunogen
This SMARCA2/BRM antibody was raised against a recombinant protein corresponding to amino acids 48-214 of mouse BRM.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
IF (Immunofluorescence)
(SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 ug/ml dilution.)
IF (Immunofluorescence)
(SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 ug/ml dilution.)
ChIP (Chromatin Immunoprecipitation)
(SMARCA2 / BRM antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
ChIP (Chromatin Immunoprecipitation)
(SMARCA2 / BRM antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Related Product Information for anti-SMARCA2/BRM antibody
Short Description: Our SMARCA2/BRM antibody (mAb)(Clone 1H7A10) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription.
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription.
Product Categories/Family for anti-SMARCA2/BRM antibody
NCBI and Uniprot Product Information
Molecular Weight
190kDa
Similar Products
Product Notes
The SMARCA2/BRM (Catalog #AAA59933) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The SMARCA2/BRM antibody (mAb)(Clone 1H7A10) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA2/BRM can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the SMARCA2/BRM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA2/BRM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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