Neuropilin-1 Active Protein

Human Neuropilin-1 Protein (ECD, His Tag)

Cat. Num. AAA258582

• Purity: >95% as determined by SDS-PAGE.
• Synonyms: Neuropilin-1; N/A; Human Neuropilin-1 Protein (ECD, His Tag); BDCA4 Protein, Human; CD304 Protein, Human; Neuropilin-1 Protein, Human; NP1 Protein, Human; NRP Protein, Human; VEGF165R Protein, Human; Neuropilin-1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence: Met1-Lys644

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Tag: C-His
• Predicted N Terminal: Phe 22
• Protein Construction: A DNA sequence encoding the human NRP1 (NP_001019799.2)(Met1-Lys644) was expressed with a polyhistidine tag at the C-terminus.
• Activity: Immobilized Recombinant Human/Cynomolgus VEGF/VEGFA/VEGF165 Protein at 2 ug/mL (100 uL/well) can bind Recombinant Human Neuropilin-1 Protein (ECD, His Tag), the EC50 is 6-18 ng/mL.
• Preparation and Storage: Store it under sterile conditions at -20 to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

(Immobilized Recombinant Human/Cynomolgus VEGF/VEGFA/VEGF165 Protein(Cat:11066-HNAH) at 2 ug/mL (100 uL/well) can bind Recombinant Human Neuropilin-1 Protein (ECD, His Tag)(Cat:AAA258582), the EC50 is 6-18 ng/mL.)

Application Data

Related Product Information for Neuropilin-1 active protein

Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

Product Categories/Family for Neuropilin-1 active protein

cd antigen; Receptor; Drug Targets; Growth Factor; VEGF Receptor; Antibody Therapy; Cancer

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human NRP1 consists of 634 amino acids and predicts a molecular mass of 71.33 kDa. It migrates as an approximately 82.01 kDa band in SDS-PAGE under reducing conditions.