IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-CD2 antibody 35.1 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 35.1 (AAA72357) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72357, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human CD2 Antibody | anti-CD2 antibody
Anti-CD2 [35.1]
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Purified. Protein A affinity purified
Synonyms
CD2; N/A; Anti-CD2 [35.1]; LFA-2; LFA-3 receptor; SRBC; Rosette receptor; T-cell surface antigen CD2; Erythrocyte receptor; T-cell surface antigen T11/Leu-5; anti-CD2 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
35.1
Specificity
This antibody recognizes an epitope on the extracellular domain of the human T cell surface protein Tp50 (CD2) between amino acid residues 39-50. The binding of this antibody does not inhibit the interaction between CD2 and CD58. CD2 is a glycoprotein belonging to the immunoglobulin superfamily. It acts as a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD2 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-CD2 antibody 35.1 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 35.1 (AAA72357) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72357, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-CD2 antibody 35.1 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 35.1 (AAA72357) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72357, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western blot using anti-CD2 antibody 35.1 . Jurkat (A) and Molt-4 (B) cells lysate, and human thymus (C) and tonsil (D) tissue lysates (35ug protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 35.1 (AAA72357) at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used, and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-CD2 antibody 35.1 . Jurkat (A) and Molt-4 (B) cells lysate, and human thymus (C) and tonsil (D) tissue lysates (35ug protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 35.1 (AAA72357) at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used, and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-CD2 antibody 35.1 . Jurkat cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 35.1 (AAA72357, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-CD2 antibody 35.1 . Jurkat cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 35.1 (AAA72357, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-CD2 antibody
The original antibody was generated by immunizing mice with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Describes the characterization of epitopes on the human T cell surface protein Tp50 using two antibodies.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Describes the characterization of epitopes on the human T cell surface protein Tp50 using two antibodies.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-CD2 antibody
References
Martin et al.
J Immunol. 1983 Jul;131(1):180-5.
J Immunol. 1983 Jul;131(1):180-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,448 Da
NCBI Official Full Name
T-cell surface antigen CD2
NCBI Official Synonym Full Names
CD2 molecule
NCBI Official Symbol
CD2
NCBI Official Synonym Symbols
T11; SRBC; LFA-2
NCBI Protein Information
T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
UniProt Protein Name
T-cell surface antigen CD2
UniProt Gene Name
CD2
UniProt Synonym Gene Names
SRBC
UniProt Entry Name
CD2_HUMAN
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Product Notes
The CD2 cd2 (Catalog #AAA72357) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD2 [35.1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the CD2 cd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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