IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2DCX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb02365,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
PD-L1 recombinant antibody
Anti-PD-L1 [B7HC0013], Mouse IgG1, kappa
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Protein A affinity purified
Synonyms
PD-L1, Recombinant Antibody; Anti-PD-L1 [B7HC0013], Mouse IgG1, kappa; PD-L1; Anti-PD-L1 [B7HC0013]; CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1; PD-L1 recombinant antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
B7HC0013
Specificity
This antibody binds human PD-L1.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for PD-L1 recombinant antibody
IHC (Immunohistochemistry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2DCX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb02365,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2DCX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb02365,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofMDA-MB-231cellswithanti-PD-L1antibodyB7HC0013(AAA72536). ImmunofluorescenceanalysisofparaformaldehydefixedMDA-MB-231cellsstainedwiththechimericrabbitIgGversionofB7HC0013(AAA72536)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72536,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofMDA-MB-231cellswithanti-PD-L1antibodyB7HC0013(AAA72536). ImmunofluorescenceanalysisofparaformaldehydefixedMDA-MB-231cellsstainedwiththechimericrabbitIgGversionofB7HC0013(AAA72536)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72536,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-PD-L1antibodyB7HC0013(AAA72536). HeLacellslysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofB7HC0013(AAA72536)at0.5ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-PD-L1antibodyB7HC0013(AAA72536). HeLacellslysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofB7HC0013(AAA72536)at0.5ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
Related Product Information for PD-L1 recombinant antibody
The original antibody was generated by panning a naive human scfv library against decreasing concentrations of free recombinant human PD-L1 (his tagged).
References
WO2014165082 N/A
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The PD-L1 cd274 (Catalog #AAA72536) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PD-L1 [B7HC0013], Mouse IgG1, kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PD-L1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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