PD-L1 recombinant antibody

PD-L1 Single Domain Antibody [F6A9]

Cat. Num. AAA40970

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Synonyms: PD-L1, Recombinant Antibody; PD-L1 Single Domain Antibody [F6A9]; PD-L1 Antibody: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1; B7 homolog 1; CD274; PD-L1 recombinant antibody

• Host: Llama
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: F6A9
• Specificity: At least three isoforms of PD-L1 are known to exist.
• Purity/Purification: PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Form/Format: Liquid; PD-L1 Antibody is supplied in PBS.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for PD-L1 recombinant antibody: ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: PD-L1 antibody was raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1.
• Research Area: Immunology, Cancer, Cell Cycle
• NCBI Organism: Homo sapiens
• Preparation and Storage: PD-L1 antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ICC (Immunocytochemistry)

(Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10ug/ml.)

FCM/FACS (Flow Cytometry)

(Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10ug/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.)

Related Product Information for PD-L1 recombinant antibody

PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.

Product Categories/Family for PD-L1 recombinant antibody

Primary Antibodies

References

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.

NCBI and Uniprot Product Information

• NCBI GI #: 7661534
• NCBI GeneID: 29126
• NCBI Accession #: NP_054862
• NCBI GenBank Nucleotide #: NM_014143.3
• UniProt Accession #: Q9NZQ7; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
• Molecular Weight: Predicted: 33kDa; Observed: 37kDa
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform a
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN