Rabbit anti-Human PD-L1 [BK58] Monoclonal Antibody | anti-PD-L1 antibody

Recombinant PD-L1 antibody [BK58]

Cat. Num. AAA296696

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: PD-L1 [BK58], Antibody; Recombinant PD-L1 antibody [BK58]; Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; PDL-1; anti-PD-L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: BK58
• Specificity: Recombinant anti-PD-L1 monoclonal antibody recognizes endogenous levels of total PD-L1 protein.
• Form/Format: Purified recombination monoclonal antibody supplied in PBS with 0.05% (W/V) Proclin300, and 0.05% BSA. This antibody is purified through a protein A column.

• Applicable Applications for anti-PD-L1 antibody: IHC (Immunohistochemistry)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Dilution: IHC: 1:1000)

IHC (Immunohiostchemistry)

(Dilution: IHC-P 1:1000Immunohistochemical analysis of paraffin-embedded human esophageal squamous carcinoma tissue using AAA296696 performed on the Abcarta FAIP-48 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. AmpSeeTM Detection Systems was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Dilution: IHC-P 1:1000Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AAA296696 performed on the Abcarta® FAIP-48 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. AmpSeeTM Detection Systems was used as the secondary antibody.)

Related Product Information for anti-PD-L1 antibody

Rabbit Monoclonal Antibody (Mab)
Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung. Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas. Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma. Additional research links PD-L1 expression to cancers associated with viral infections. Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Product Categories/Family for anti-PD-L1 antibody

New Antibodies; Primary Antibodies; Cancer; Immunology

References

Dong H., et al.Nat. Med. 5:1365-1369 (1999). Freeman G.J., et al.J. Exp. Med. 192:1027-1034 (2000). He X.-H., et al.Acta Pharmacol. Sin. 26:462-468 (2005). Chi X.-Y., et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases. Ota T., et al.Nat. Genet. 36:40-45 (2004).Dong, H. et al. (1999) Nat Med 5, 1365-9.Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.Dong, H. et al. (2002) Nat Med 8, 793-800.Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.Wimberly, H. et al. (2014) Cancer Immunol Res,.

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NM_001267706.1
• UniProt Accession #: Q9NZQ7; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
• Molecular Weight: 33,275 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN