Standard Curve (Sample)
Human PD-1 ELISA Kit | PD-1 elisa kit
Human PD-1 ELISA Kit
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human
Applications
ELISA
Synonyms
PD-1; N/A; Human PD-1 ELISA Kit; Programmed cell death protein 1; hPD-1; PDCD1; CD279; PD-1 elisa kit
Reactivity
Human
Specificity
Human PD-1 ELISA Kit has high sensitivity and excellent specificity for detection of Human PD-1. No significant cross-reactivity or interference between Human PD-1 and analogues was observed.
Assay Type
Sandwich
Samples
Serum, Plasma, Cell culture supernatants
Detection Range
3.9 pg/mL-250 pg/mL
Sensitivity
3.9 pg/mL
Applicable Applications for PD-1 elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision: The CV (%)?10%.
Inter-assay Precision
Inter-assay Precision :The CV (%)?12%
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PD-1 elisa kit
Principle of the Assay: PD-1 (Programmed cell death protein 1, also known as CD279), is a type I transmembrane protein composed of 288 amino acids, belonging to the B7-CD28 family. PD-1 can be expressed in activated CD4+ T cells, CD8 T cells, B cells, natural killer T cells, monocytes and dendritic cells. In addition, studies have shown that PD-1 is also expressed in regulatory T cells (Treg), and can promote the proliferation of Treg cells and inhibit the immune response. PD-1 is the receptor of PD-L1. After binding with PD-L1, it can recruit tyrosine phosphatase SHP-2 through immunoreceptor tyrosine-based swith motif (ITSM), and then dephosphorylate multiple key molecules in TCR signaling pathway, inhibit the proliferation and activity of CD4T and CD8T cells, and negatively regulate the immune response process of the body.Human PD-1 ELISA Kit employs a double antibody sandwich method to quantitate Human PD-1 in samples. An antibody specific for Human PD-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human PD-1 present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human PD-1 is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human PD-1 concentration is proportional to the OD450 nm value.
Product Categories/Family for PD-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,647 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
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Product Notes
The Human PD-1 pdcd1 (Catalog #AAA332602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332602 ELISA Kit recognizes Human PD-1. AAA Biotech's PD-1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the PD-1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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