Rabbit anti-Human SIRT1 Polyclonal Antibody | anti-SIRT1 antibody

SIRT1 Antibody

Cat. Num. AAA307370

• Gene Names: SIRT1; SIR2; hSIR2; SIR2L1
• Reactivity: Human
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SIRT1, Antibody; SIRT1 Antibody; SIRT1; SIR2L1; anti-SIRT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SIRT1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 452

• Applicable Applications for anti-SIRT1 antibody: ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human SIRT1.
• Target Name: SIRT1
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cell using SIRT1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat brain using SIRT1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human brain cancer using SIRT1 antibody at dilution of 1:100 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using SIRT1 antibody.)

Related Product Information for anti-SIRT1 antibody

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARg (7), and the PPARg coactivator-1a (PGC-1a) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARg and PGC-1a regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).

Product Categories/Family for anti-SIRT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 215982798
• NCBI GeneID: 23411
• NCBI Accession #: NP_001135970.1
• NCBI GenBank Nucleotide #: NM_001142498.1
• UniProt Accession #: Q96EB6; Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0
• Molecular Weight: 61,066 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform b
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: SIRT1
• NCBI Official Synonym Symbols: SIR2; hSIR2; SIR2L1
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• UniProt Gene Name: SIRT1
• UniProt Synonym Gene Names: SIR2L1; hSIRT1; hSIR2; 75SirT1
• UniProt Entry Name: SIR1_HUMAN