Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody. Lane 1: MECP2 transfected lysate (52.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in NIH/3T3.)

WB (Western Blot) (MECP2 monoclonal antibody Western Blot analysis of MECP2 expression in MCF-7,)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in rat muscle.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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MECP2, Monoclonal Antibody (Cat# AAA25452)

Full Name

MECP2 (Methyl-CpG-binding Protein 2, MeCp-2 Protein, MeCp2, DKFZp686A24160) (HRP)

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GSTZ1 on HepG2 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of GSTZ1 expression in transfected 293T cell line by GSTZ1 monoclonal antibody. Lane 1: GSTZ1 transfected lysate (24.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in NIH/3T3.)

WB (Western Blot) (GSTZ1 monoclonal antibody, Western Blot analysis of GSTZ1 expression in HepG2.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)

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GSTZ1, Monoclonal Antibody (Cat# AAA25708)

Full Name

GSTZ1 (Glutathione S-transferase zeta 1, Glutathione S-Transferase zeta 1, GSTZ1-1, Maleylacetoacetate Isomerase, MAAI, MAI, MGC2029) (PE)

Gene Names

GSTZ1; MAI; MAAI; MAAID; GSTZ1-1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

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UBTF, Monoclonal Antibody (Cat# AAA25964)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (AP)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug TTC11/FIS1 antibody (AAA28524). Western blot was performed from the immunoprecipitate using TTC11/FIS1 (AAA28524) at a dilution of 1:2000.)

ICC (Immunocytochemistry) (Confocal imaging of U-2 OS cells using [KO Validated] TTC11/FIS1 Rabbit mAb (AAA28524,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] TTC11/FIS1 Rabbit mAb (AAA28524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] TTC11/FIS1 Rabbit mAb (AAA28524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using [KO Validated] TTC11/FIS1 Rabbit mAb (AAA28524) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa(KO) cells, using [KO Validated] TTC11/FIS1 Rabbit mAb (AAA28524) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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TTC11/FIS1, Monoclonal Antibody (Cat# AAA28524)

Full Name

[KO Validated] TTC11/FIS1 Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with alpha smooth muscle Actin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: Mouse heart)

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alpha Smooth Muscle Actin, Monoclonal Antibody (Cat# AAA30058)

Full Name

alpha Smooth Muscle Actin Antibody

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody. Lane 1: RPS6KA2 transfected lysate (83.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RPS6KA2 monoclonal antibody, Western Blot analysis of RPS6KA2 expression in Hela NE.)

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RSK3, Monoclonal Antibody (Cat# AAA24939)

Full Name

RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-

Gene Names

RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

WB (Western Blot) (Western blot analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MDM2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5ug/ml])

WB (Western Blot) (Western Blot analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody. Lane 1: MDM2 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MDM2, Monoclonal Antibody (Cat# AAA25451)

Full Name

MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Hdm2, Oncoprotein Mdm2, p53-binding Protein Mdm2) (HRP)

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (A-549 cells were stained with POU5F1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on A-549 cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 monoclonal antibody (M05), clone 1B11.Lane 1: POU5F1 transfected lysate(18.3 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (POU5F1 monoclonal antibody (M05), clone 1B11 Western Blot analysis of POU5F1 expression in HepG2.)

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POU5F1, Monoclonal Antibody (Cat# AAA25963)

Full Name

POU5F1 (POU Class 5 Homeobox 1, MGC22487, OCT3, OCT4, OTF3, OTF4) (AP)

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IHC (Immunohistchemistry) (Immunochemical staining CD3 in cynomolgus lymph node with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CD3 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

FCM (Flow Cytometry) (Flow cytometric analysis of Human CD3E expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD3E, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

IHC (Immunohistochemistry) (Immunochemical staining of human CD3E in cynomolgus macaque lymphnode with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human CD3E in cynomolgus macaque tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining CD3E in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

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CD3D & CD3E Heterodimer, Monoclonal Antibody (Cat# AAA27755)

Full Name

Recombinant Anti-CD3D & CD3E Heterodimer Antibody, Rabbit Monoclonal

Gene Names

CD3D; T3D; IMD19; CD3-DELTA

Reactivity

Human, Cynomolgus

Applications

ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)

Purity

Protein A

Pricing

$205/0.02 mL $375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse testis using Calmegin Rabbit mAb (AAA28520) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat testis using Calmegin Rabbit mAb (AAA28520) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis using Calmegin Rabbit mAb (AAA28520) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis using Calmegin Rabbit mAb (AAA28520) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from PC-3 cells, using Calmegin Rabbit mAb (AAA28520) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of various lysates using Calmegin Rabbit mAb (AAA28520) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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Calmegin, Monoclonal Antibody (Cat# AAA28520)

Full Name

Calmegin Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

Affinity purification

Pricing

$255/0.02 mL $435/0.1 mL $835/2x0.1 mL $1,215/3x0.1 mL $1,570/4x0.1 mL $1,685/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HARS mAb. [Lot No. HARSA6-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-HARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. HARSA6-2])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-HARS mAb. [Lot No. HARSA6-1]Predicted molecular weight: 57 kDa)

WB (Western Blot) (Detection of HARS by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. HARSA6-2]Predicted molecular weight: 57 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-HARS monoclonal antibody.)

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HARS, Monoclonal Antibody (Cat# AAA10601)

Full Name

Mouse monoclonal antibody Anti-Human HARS

Gene Names

HARS; HRS; USH3B

Reactivity

Human

Applications

Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Pricing

$575/0.1 mg $1,110/2x0.1 mg $1,615/3x0.1 mg $2,090/4x0.1 mg $2,325/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell. [antibody concentration 20ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in NIH/3T3.)

WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in Raw 264.7.)

WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in PC-12.)

WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in HeLa.)

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RPS7, Monoclonal Antibody (Cat# AAA24937)

Full Name

RPS7 (40S Ribosomal Protein S7) (Biotin)

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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GROS1, Monoclonal Antibody (Cat# AAA25705)

Full Name

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (PE)

Gene Names

P3H1; OI8; GROS1; LEPRE1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6. Western Blot analysis of SP1 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6 Western Blot analysis of SP1 expression in IMR-32 (Cat # L008V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

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SP1, Monoclonal Antibody (Cat# AAA26473)

Full Name

SP1 (Sp1 Transcription Factor) (HRP)

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

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CD274, Monoclonal Antibody (Cat# AAA26729)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (Biotin)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Flow Cytometry (FC/FACS)

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$780/0.1 mL $1,510/2x0.1 mL $2,200/3x0.1 mL $2,850/4x0.1 mL $3,275/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody (AAA28521) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

ICC (Immunocytochemistry) (Confocal imaging of C6 cells using SP1 Rabbit mAb (AAA28521,at dilution of 1:100) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using SP1 Rabbit mAb (AAA28521) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using SP1 Rabbit mAb (AAA28521) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb (AAA28521). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb (AAA28521). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).)

Application Data (CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10? Hep G2 cells with 1 ug SP1 Rabbit mAb (AAA28521), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2), as shown in figure.)

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SP1, Monoclonal Antibody (Cat# AAA28521)

Full Name

SP1 Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq, CUT&Tag

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STAT3 mAb. [Lot No. STAAD22A-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-STAT3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. STAAD22A-2])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-STAT3 mAb. [Lot No. STAAD22A-2]Predicted molecular weight: 87 kDa)

WB (Western Blot) (Detection of STAT3 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. STAAD22A-2]Predicted molecular weight: 87 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-STAT3 monoclonal antibody.)

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STAT3, Monoclonal Antibody (Cat# AAA10599)

Full Name

Mouse monoclonal antibody Anti-Human STAT3

Gene Names

STAT3; APRF; HIES; ADMIO

Reactivity

Human

Applications

ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Pricing

$575/0.1 mg $1,110/2x0.1 mg $1,615/3x0.1 mg $2,090/4x0.1 mg $2,325/5x0.1 mg

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of C2C12 cells, either LY294002-treated (left) or insulin-treated (right), using AAA14695 (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

FCM (Flow Cytometry) (Flow cytometric analysis of untreated Jurkat cells using AAA14695 (blue) compared to a nonspecific negative control antibody (red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis using AAA14695 on SignalSlide™ Phospho-Akt (Ser473) IHC Controls (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma using AAA14695 in the presence of control peptide (left) or Akt (pan) Blocking Peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3, C6 and COS cells using AAA14695.)

WB (Western Blot) (Western blot analysis of recombinant Akt1, Akt2, Akt3 and GST proteins using AAA14695 (upper) and GST (91G1) Rabbit mAb (lower).)

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Akt, pan, Monoclonal Antibody (Cat# AAA14695)

Full Name

Akt, pan (Rac PKa, PKBa)

Gene Names

Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Ascites
Ascites

Pricing

$745/0.1 mL $1,440/2x0.1 mL $2,100/3x0.1 mL $2,720/4x0.1 mL $3,125/5x0.1 mL

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WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged CDX4 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in Raw 264.7.)

WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in PC-12.)

WB (Western Blot) (CDX4 monoclonal antibody Western Blot analysis of CDX4 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CDX4, Monoclonal Antibody (Cat# AAA24167)

Full Name

CDX4 (Homeobox Protein CDX-4, Caudal-type Homeobox Protein 4) (AP)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])

WB (Western Blot) (RUNX1 monoclonal antibody (M06), clone 2C10 Western Blot analysis of RUNX1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])

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RUNX1, Monoclonal Antibody (Cat# AAA26215)

Full Name

RUNX1 (Runt-Related Transcription Factor 1, AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB) (Biotin)

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa (Cat # L013V1).)

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RIPK2, Monoclonal Antibody (Cat# AAA26471)

Full Name

RIPK2 (Receptor-Interacting Serine-Threonine Kinase 2, CARD3, CARDIAK, CCK, GIG30, RICK, RIP2) (HRP)

Gene Names

RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and ATG7 knockdown (KD) HeLa cells using [KD Validated] ATG7 Rabbit mAb (AAA28516) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of various lysates using [KD Validated] ATG7 Rabbit mAb (AAA28516) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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ATG7, Monoclonal Antibody (Cat# AAA28516)

Full Name

[KD Validated] ATG7 Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), ELISA (EIA)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312: RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD312: Biotin)

Application Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD312 : Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD312: FITC)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312)

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CD312, Monoclonal Antibody (Cat# AAA12143)

Full Name

MOUSE ANTI HUMAN CD312:RPE

Gene Names

EMR2; CD312

Applications

Flow cytometry (FC/FACS)

Pricing

$645/100 Tests $1,245/2x100 Tests $1,815/3x100 Tests $2,350/4x100 Tests $2,650/5x100 Tests

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SDS-PAGE (SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot) (Western Blot Analysis of HEP-2 and PC3 cell lysates using HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed HeLa cells labeling HSP60 with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red))

IF (Immunofluorescence) (Immunofluorescence Analysis of human MCF-7 cells labeling GPI with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

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HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA23919)

Full Name

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Gene Names

HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60

Reactivity

Human

Applications

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])

WB (Western Blot) (Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (85.36kD).)

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PGM3, Monoclonal Antibody (Cat# AAA25199)

Full Name

PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) (FITC)

Gene Names

PGM3; AGM1; PAGM; IMD23; PGM 3

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

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HCLS1, Monoclonal Antibody (Cat# AAA25711)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (PE)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A Mouse mAb diluted at 1:200.)

WB (Western Blot) (Western blot analysis of lysates from 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue Lysate using LC3A Mouse mAb diluted at 1:1000.)

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LC3A, Monoclonal Antibody (Cat# AAA30831)

Full Name

LC3A Mouse Monoclonal Antibody (5G10)

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$270/0.04 mL $360/0.1 mL $515/0.2 mL $990/2x0.2 mL $1,440/3x0.2 mL $1,865/4x0.2 mL $2,050/5x0.2 mL

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Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Published clone specific image: Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 (left) and ED9 (right) expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE)

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CD172a, Monoclonal Antibody (Cat# AAA11888)

Full Name

MOUSE ANTI RAT CD172a:FITC

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Flow cytometry (FC/FACS)

Pricing

$515/0.1 mg $990/2x0.1 mg $1,440/3x0.1 mg $1,865/4x0.1 mg $2,065/5x0.1 mg

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Application Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13)

Application Data (Staining of J774 cells with Rat anti Mouse CD13:Alexa Fluor 488 (AAA12144A488))

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

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CD13, Monoclonal Antibody (Cat# AAA12144)

Full Name

RAT ANTI MOUSE CD13

Gene Names

Anpep; Apn; AP-M; AP-N; Cd13; P150

Applications

Immunohistology Frozen, Flow Cytometry (FC)

Purity

Affinity chromatography on Protein G from tissue culture supernatant

Pricing

$620/0.25 mg $1,195/2x0.25 mg $1,740/3x0.25 mg $2,255/4x0.25 mg $2,530/5x0.25 mg

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

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UBTF, Monoclonal Antibody (Cat# AAA26230)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (Biotin)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data (Staining of human peripheral blood lymphocytes with C2253-02D.)

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CD2, Monoclonal Antibody (Cat# AAA26742)

Full Name

CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (FITC)

Reactivity

Human, Mouse

Applications

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$960/0.1 mL $1,860/2x0.1 mL $2,710/3x0.1 mL $3,515/4x0.1 mL $4,080/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining TIA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TIA1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TIA1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TIA1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution.)

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TIA1, Monoclonal Antibody (Cat# AAA30326)

Full Name

TIA1 Antibody

Gene Names

TIA1; WDM; TIA-1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TWIST mAb. [Lot No. Twist2C1a-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TWIST mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. Twist2C1a-1])

WB (Western Blot) (Detection of TWIST by Western blot.Samples: Whole cell lysate from human A2058 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. Twist2C1a-1]Predicted molecular weight: 20 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-TWIST monoclonal antibody.)

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TWIST, Monoclonal Antibody (Cat# AAA10606)

Full Name

Mouse monoclonal antibody Anti-Human TWIST

Gene Names

TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38

Reactivity

Human

Applications

Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Pricing

$575/0.1 mg $1,110/2x0.1 mg $1,615/3x0.1 mg $2,090/4x0.1 mg $2,325/5x0.1 mg

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Medium power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8)

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11886)

Full Name

RAT ANTI MOUSE CD8 ALPHA:FITC

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

Flow cytometry (FC/FACS)

Pricing

$730/0.5 mg $1,410/2x0.5 mg $2,055/3x0.5 mg $2,665/4x0.5 mg $3,030/5x0.5 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488 (AAA12142A488))

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312: RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD312: Biotin)

Application Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD312 : Alexa Fluor 647 (AAA12142A647))

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD312: FITC)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312)

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CD312, Monoclonal Antibody (Cat# AAA12142)

Full Name

MOUSE ANTI HUMAN CD312

Gene Names

EMR2; CD312

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Pricing

$620/0.2 mg $1,195/2x0.2 mg $1,740/3x0.2 mg $2,255/4x0.2 mg $2,530/5x0.2 mg

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Application Data (Detection limit for recombinant GST tagged S100A4 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A4 on HeLa cell. [antibody concentration 15ug/ml])

WB (Western Blot) (Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody. Lane 1: S100A4 transfected lysate (11.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in NIH/3T3.)

WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (37.22kD))

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S100A4, Monoclonal Antibody (Cat# AAA24942)

Full Name

S100A4 (Protein S100-A4, Calvasculin, Metastasin, Placental Calcium-binding Protein, Protein Mts1, S100 Calcium-binding Protein A4, CAPL, MTS1) (Biotin)

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human, Mouse

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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HADHSC, Monoclonal Antibody (Cat# AAA25710)

Full Name

HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co

Gene Names

HADH; HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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SDS-PAGE (SDS-PAGE Analysis Purified ARF1 Mouse Monoclonal Antibody (ARF1/2117). Confirmation of Integrity and Purity of the Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

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ARF1, Monoclonal Antibody (Cat# AAA23921)

Full Name

ARF1 (Golgi Apparatus Marker)

Gene Names

ARF1; PVNH8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC) Paraffin

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRYM, Monoclonal Antibody (Cat# AAA24177)

Full Name

CRYM (Thiomorpholine-carboxylate Dehydrogenase, Mu-crystallin Homolog, NADP-regulated Thyroid-hormone-binding Protein, Ketimine Reductase, THBP) (AP)

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (TRIM33 monoclonal antibody, Western Blot analysis of TRIM33 expression in Hela NE)

WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in 293)

Application Data (Detection limit for recombinant GST tagged TRIM33 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM33 on HeLa cell. [antibody concentration 30ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM33 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in PC-12)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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TRIM33, Monoclonal Antibody (Cat# AAA24689)

Full Name

TRIM33 (E3 Ubiquitin-protein Ligase TRIM33, Ectodermin Homolog, RET-fused Gene 7 Protein, Protein Rfg7, Transcription Intermediary Factor 1-gamma, TIF1-gamma, Tripartite Motif-containing Protein 33, KIAA1113, RFG7, TIF1G) APC

Gene Names

TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

Application Data (Published customer image: CD11b immunoreactive (red) microglia in the motor cortex following cervical corticospinal tract injury. Representative images of motor cortex - a, b: one week (deconvolved images); c, d: two weeks following corticospinal tract injury. The corticospinal neurons were retrogradely labelled with fluorogold applied to the spinal cord at the time of injury. There is little sign of microglial activation. The inset in (c) shows a CD6-positive T-cell in the motor cortex, but no accumulation of such cells was detected.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b)

Application Data (Published customer image: Microglia in the red nucleus (a-d and inset) and motor cortex (e, f) 4 weeks after nerve graft implantation into the rubrospinal tract and cervical dorsal columns respectively. Neurons with regenerating axons were retrogradely labelled with fluorogold. Microglia established close contacts with regenerating rubrospinal neurons (arrows), which also expressed ATF3 (inset). No retrogradely labelled corticospinal neurons were detected (e and f) and there was no microglial activation in the motor cortex. (a-c) are non-deconvolved and (d-f plus inset) are deconvolved images. The scale bar in (a) also applies to (b).From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD11b:Alexa Fluor 488)

Application Data (Published customer image: Rubrospinal injury produces little microglial activation and no accumulation of T-cells in the red nucleus. a, b: Representative images show there is little difference in CD11b immunoreactivity for microglia in the red nucleus (*) one week following axotomy. c, d: confocal images of beta thymosin immunoreactive microglia in the red nucleus 3 weeks after injury. e: a rare CD6-positive T lymphocyte in the red nucleus of an unoperated rat. f: one week after injury no T lymphocytes can be identified in the red nucleus around the axotomized rubrospinal neurons, which can be recognised by their expression of ATF3 (green). Neuronal cytoplasm has been visualised by high gain in the red signal; this does not represent CD6 signal.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b:RPE-Alexa Fluor 647)

Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power)

Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power)

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CD11b, Monoclonal Antibody (Cat# AAA12146)

Full Name

MOUSE ANTI RAT CD11b:FITC

Gene Names

ITGAM; CD11B

Applications

Flow cytometry (FC/FACS)

Pricing

$515/0.1 mg $990/2x0.1 mg $1,440/3x0.1 mg $1,865/4x0.1 mg $2,065/5x0.1 mg

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

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Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17783)

Full Name

Hsp70/Hsc70 Antibody: ATTO 594

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

Westen Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Protein-binding Assay

Pricing

$640/0.2 mg $1,235/2x0.2 mg $1,800/3x0.2 mg $2,330/4x0.2 mg $2,565/5x0.2 mg

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WB (Western Blot) (Western Blot analysis of CTNS expression in PC-12.)

WB (Western Blot) (Western Blot detection against immunogen using (36.74kD).)

WB (Western Blot) (Western Blot analysis of CTNS expression in Raw 264.7)

WB (Western Blot) (Western Blot analysis of CTNS expression in HepG2)

WB (Western Blot) (Western Blot analysis of CTNS expression in human liver)

Application Data (Detection limit is ~0.3ng/ml using as a capture antibody.)

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Cystinosin, Monoclonal Antibody (Cat# AAA24183)

Full Name

Cystinosin (CTNS) (AP)

Gene Names

CTNS; PQLC4; SLC66A4; CTNS-LSB

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in NIH/3T3.)

WB (Western Blot) (UBQLN2 monoclonal antibody, Western Blot analysis of UBQLN2 expression in A-431.)

Application Data (Detection limit for recombinant GST tagged UBQLN2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBQLN2 on A-431 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in Raw 264.7.)

WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

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Ubiquilin-2, Monoclonal Antibody (Cat# AAA24695)

Full Name

Ubiquilin-2 (UBQLN2, Chap1, DSK2 Homolog, HRIHFB2157, N4BP4, Protein Linking IAP with Cytoskeleton 2, PLIC2, PLIC-2, hPLIC-2, Ubiquitin-like Product Chap1/Dsk2) APC

Gene Names

UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MUTYH, Monoclonal Antibody (Cat# AAA25463)

Full Name

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (HRP)

Gene Names

MUTYH; MYH

Reactivity

Human

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562 (Cat # L009V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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TCF7L2, Monoclonal Antibody (Cat# AAA26487)

Full Name

TCF7L2 (Transcription Factor 7-like 2 (T-Cell Specific, HMG-Box), TCF-4, TCF4) (HRP)

Gene Names

TCF7L2; TCF4; TCF-4

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TNF Receptor II on different cells lysates using anti-TNF Receptor II antibody at 1/500 dilution. Positive control�� Line 1: MCF-7 Line 2: Jurkat)

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TNF II, Monoclonal Antibody (Cat# AAA30327)

Full Name

TNF Receptor II Antibody

Gene Names

TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ASB10 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (ASB10 monoclonal antibody, Western Blot analysis of ASB10 expression in A-431.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in Raw 264.7.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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ASB10, Monoclonal Antibody (Cat# AAA24436)

Full Name

ASB10 (Ankyrin Repeat and SOCS Box Protein 10, ASB-10) APC

Gene Names

ASB10; GLC1F

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))

Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)

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PIK3R1, Monoclonal Antibody (Cat# AAA25204)

Full Name

PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (FITC)

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 40ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody. Lane 1: MPG transfected lysate (32.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MPG monoclonal antibody Western Blot analysis of MPG expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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MPG, Monoclonal Antibody (Cat# AAA25460)

Full Name

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (HRP)

Gene Names

MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.