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Application Data (Detection limit for recombinant GST tagged TCEA3 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (TCEA3 monoclonal antibody (M08), clone 4E11 Western Blot analysis of TCEA3 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Tubulin alpha-1B/K-alpha-1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Tubulin alpha-1B/K-alpha-1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Tubulin alpha-1B/K-alpha-1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Tubulin alpha-1B/K-alpha-1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Tubulin alpha-1B/K-alpha-1 Rabbit mAb at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using alpha Tubulin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
ICC (Immunocytochemistry) (ICC staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H1.0 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1, 000 dilution.)
WB (Western Blot) (LZTFL1 monoclonal antibody, Western Blot analysis of LZTFL1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoDetection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.clonal antibody to LZTFL1 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LZTFL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of LZTFL1 expression in transfected 293T cell line by LZTFL1 monoclonal antibody. Lane 1: LZTFL1 transfected lysate (34.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody. Lane 1: ASB9 transfected lysate (31.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (53.83kD).)
Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FOXA1 monoclonal antibody (M02), clone 1B1 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining MEK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-MEK3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MEK3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MEK3 on Hela lysates using anti-MEK3 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CSTF3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (37.07kD).)
FCM (Flow Cytometry) (Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [10H6]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [10H6]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [10H6]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml)
WB (Western Blot) (Detection of human CD247/CD3Z by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Simple Western (Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)
IP (Immunoprecipitation) (Detection of human CD247/CD3Z by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3) used for IP at 20 ul/mg lysate. CD247/CD3Z was also immunoprecipitated by a previous lot of this antibody (AAA23794 lot 2). For blotting immunoprecipitated CD247/CD3Z, AAA23794 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human CD247/CD3Z (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human CD247/CD3Z in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD247/CD3Z in FFPE appendix by immunohistochemistry. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CD247/CD3Z by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562 (Cat # L009V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.)
IF (Immunofluorescence) (Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))
FCM (Flow Cytometry) (Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
WB (Western Blot) (Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).)
WB (Western Blot) (Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
ICC (Immunocytochemistry) (ICC staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2)
Strength of Signal (Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
FCM (Flow Cytometry) (Flow Cytometry of human Vimentin on Jurkat Cells, Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).)
SDS-PAGE (SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). Confirmation of Integrity and Purity of Antibody.)
WB (Western Blot) (Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452))
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452))
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (68.53kD).)
WB (Western Blot) (Western blot analysis of GSC over-expressed 293 cell line, cotransfected with GSC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSC monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GSC is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody. Lane 1: GSC transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in SJCRH30.)
WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (37.88kD).)
WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (39.93kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with CDC20 rabbit purified polyclonal 1:1200 and BUB1B mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BUB1B is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody. Lane 1: BUB1B transfected lysate (119.5kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged WWP1 is 0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of WWP1 transfected lysate using WWP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with WWP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody Lane 1: WWP1 transfected lysate (105.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431 (Cat # L015V1).)
WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged ABL2 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (ABL2 monoclonal antibody (M09), clone 5C6 Western Blot analysis of ABL2 expression in K-562 (Cat # L009V1).)
FCM (Flow Cytometry) (Flow cytometric analysis of PANC-1 cells with AP2M1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining AP2M1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AP2M1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AP2M1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Detection of human Granzyme B by western blot. Samples: Whole cell lysate (15 ug) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinant monoclonal antibody (AAA23797 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human Granzyme B by western blot. Samples: Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody .)
IHC (Immunohistochemistry) (Detection of human Granzyme B (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human Granzyme B by immunocytochemistry. Sample: FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human Granzyme B (shaded) in SR cells by flow cytometry. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody (AAA23797) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged PLXNA2 is 0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of PLXNA2 expression in transfected 293T cell line by PLXNA2 monoclonal antibody. Lane 1: PLXNA2 transfected lysate (Predicted MW: 18.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in NIH/3T3.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in Raw 264.7.)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in HepG2)
WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (43.78kD).)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
WB (Western Blot) (RUNX1 monoclonal antibody (M06), clone 2C10 Western Blot analysis of RUNX1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution.)
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)
Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. High power)
Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Medium power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)
Application Data (Staining of KM-H2 cells with Mouse anti Human CD83: Alexa Fluor 647)
Application Data (Staining of KM-H2 cells with Mouse anti Human CD83)
Application Data (Staining of KM-H2 cell line with Mouse anti Human CD83:FITC)
Application Data (Detection limit for recombinant GST tagged MEOX2 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml])
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in Hela NE.)
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in PC-12.)
WB (Western Blot) (MEOX2 monoclonal antibody Western Blot analysis of MEOX2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (59.07kD).)
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SNX1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNX1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SNX1 expression in transfected 293T cell line by SNX1 monoclonal antibody. Lane 1: SNX1 transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SNX1 monoclonal antibody, Western Blot analysis of SNX1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate (105.4 KDa).Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with Creatine kinase B type antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Creatine kinase B type in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse brain Lane 3: Rat brain Lane 4: 293)
Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody. Lane 1: MECP2 transfected lysate (52.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in NIH/3T3.)
WB (Western Blot) (MECP2 monoclonal antibody Western Blot analysis of MECP2 expression in MCF-7,)
WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in rat muscle.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western Blot analysis of DHFR expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence on HeLa cells using 125814 (10ug/ml).)
Application Data (Detection limit for 125814 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of DHFR expression in transfected 293T cell line using 125814. Lane 1: DHFR transfected lysate (21.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of DHFR expression in PC-12 using 125814.)
WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ABCF2 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody: Lane 1: ABCF2 transfected lysate (68.53kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABCF2 monoclonal antibody Western Blot analysis of ABCF2 expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
SNAI2 (Zinc Finger Protein SNAI2, Neural Crest Transcription Factor Slug, Protein Snail Homolog 2, SLUG, SLUGH, MGC10182) (PE)
Gene Names
SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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