Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (Detection of human MED1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

WB (Western Blot) (Detection of mouse MED1 by western blot. Samples: Whole cell lysate (10 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

IP (Immunoprecipitation) (Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used for IP at 20 ul/mg lysate. MED1 was also immunoprecipitated by a previous lot of this antibody (AAA23807-1) and rabbit anti-MED1 antibody For blotting immunoprecipitated MED1, AAA23807 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human MED1 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of mouse MED1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human MED1 in FFPE HCT 116 cells by immunocytochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of mouse MED1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human MED1 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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MED1, Monoclonal Recombinant Antibody (Cat# AAA23807)

Full Name

Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]

Gene Names

MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.02 mL $450/0.1 mL $865/2x0.1 mL $930/5x0.02 mL $1,255/3x0.1 mL $1,625/4x0.1 mL $1,765/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody.Lane 1: RPS6KA2 transfected lysate (83.2kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (RPS6KA2 monoclonal antibody, Western Blot analysis of RPS6KA2 expression in Hela NE.)

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RSK3, Monoclonal Antibody (Cat# AAA24063)

Full Name

RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-

Gene Names

RPS6KA2; RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

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WB (Western Blot) (F3 monoclonal antibody, Western Blot analysis of F3 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with F7 rabbit purified polyclonal 1:1200 and F3 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Application Data (Detection limit for recombinant GST tagged F3 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of F3 transfected lysate using F3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F3 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody. Lane 1: F3 transfected lysate (33.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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CD142, Monoclonal Antibody (Cat# AAA25343)

Full Name

CD142 (CD142 Antigen, Coagulation Factor III, F3, Tissue Factor, TF, TFA, Thromboplastin) (HRP)

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M04), clone 3D2 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA26623)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (PE)

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Published customer image: Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37 degree C for 1 hour. **P)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD11b, Monoclonal Antibody (Cat# AAA12238)

Full Name

MOUSE ANTI RAT CD11b

Gene Names

ITGAM; CD11B

Applications

Immunohistology Frozen*

Pricing

$590/0.25 mg $1,140/2x0.25 mg $1,655/3x0.25 mg $2,145/4x0.25 mg $2,390/5x0.25 mg

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Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD169, Monoclonal Antibody (Cat# AAA12204)

Full Name

RAT ANTI MOUSE CD169

Gene Names

Siglec1; Sn; Cd169; Siglec-1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

$620/0.2 mg $1,195/2x0.2 mg $1,740/3x0.2 mg $2,255/4x0.2 mg $2,530/5x0.2 mg

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Application Data (Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm))

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power)

Application Data (Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm)

Application Data (Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)

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CD68, Monoclonal Antibody (Cat# AAA12148)

Full Name

MOUSE ANTI RAT CD68:FITC

Applications

Flow cytometry (FC/FACS)*

Pricing

$740/0.1 mg $1,430/2x0.1 mg $2,085/3x0.1 mg $2,700/4x0.1 mg $3,065/5x0.1 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Azide Free)

Application Data (Staining of human peripheral blood monocytes using Mouse anti Human CD38:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE-Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Alexa Fluor 647)

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CD38, Monoclonal Antibody (Cat# AAA11845)

Full Name

MOUSE ANTI HUMAN CD38:Preservative Free

Gene Names

CD38; T10; ADPRC 1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Pricing

$865/1 mg $1,675/2x1 mg $2,440/3x1 mg $3,160/4x1 mg $3,630/5x1 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Azide Free)

Application Data (Staining of human peripheral blood monocytes using Mouse anti Human CD38:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE-Alexa Fluor 750 (AAA11913P750))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Alexa Fluor 647 (AAA11913A647))

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CD38, Monoclonal Antibody (Cat# AAA11913)

Full Name

MOUSE ANTI HUMAN CD38

Gene Names

CD38; T10; ADPRC 1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Pricing

$560/0.2 mg $1,080/2x0.2 mg $1,570/3x0.2 mg $2,035/4x0.2 mg $2,275/5x0.2 mg

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WB (Western Blot) (Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GABPA monoclonal antibody.)

Application Data (Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

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GABPA, Monoclonal Antibody (Cat# AAA26110)

Full Name

GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kD, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A) (APC)

Gene Names

GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07

Applications

Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])

WB (Western Blot) (ROCK2 monoclonal antibody (M02), clone 1E12 Western Blot analysis of ROCK2 expression in Hela S3 NE (Cat # L013V3).)

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ROCK2, Monoclonal Antibody (Cat# AAA26622)

Full Name

ROCK2 (Rho-Associated, coiled-coil Containing Protein Kinase 2, KIAA0619) (PE)

Gene Names

ROCK2; ROCK-II

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

Application Data

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM4) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM4) on K562-BCMA (Red histogram)(K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line)(Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA30718)

Full Name

Anti-BCMA antibody (DM4), Rabbit mAb

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$230/0.01 mg $840/0.1 mg $1,625/2x0.1 mg $2,370/3x0.1 mg $3,070/4x0.1 mg $3,550/5x0.1 mg

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ELISA (Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.Green: TIGIT Antibody [10B1]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.Green: TIGIT Antibody [10B1]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

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TIGIT, Monoclonal Antibody (Cat# AAA11007)

Full Name

TIGIT Antibody [10B1]

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Protein A purified

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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WB (Western Blot) (MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody. Lane 1: MKRN2 transfected lysate (46.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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MKRN2, Monoclonal Antibody (Cat# AAA24572)

Full Name

MKRN2 (RNF62, Probable E3 Ubiquitin-protein Ligase Makorin-2, RING Finger Protein 62, HSPC070) APC

Gene Names

MKRN2; RNF62; HSPC070

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

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EFHD1, Monoclonal Antibody (Cat# AAA25084)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (FITC)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (ACSL5 monoclonal antibody, Western Blot analysis of ACSL5 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged ACSL5 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ACSL5 transfected lysate using ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody. Lane 1: ACSL5 transfected lysate (82.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.67kD).)

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ACSL5, Monoclonal Antibody (Cat# AAA25596)

Full Name

ACSL5 (Long-chain-fatty-acid-CoA Ligase 5, Long-chain acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250) (PE)

Gene Names

ACSL5; ACS2; ACS5; FACL5

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Application Data (Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (GABPA monoclonal antibody (M07), clone 5C8 Western Blot analysis of GABPA expression in Hela S3 NE (Cat # L013V3).)

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GABPA, Monoclonal Antibody (Cat# AAA26108)

Full Name

GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kD, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A) (APC)

Gene Names

GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])

Application Data (Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

WB (Western Blot) (PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE (Cat # L013V3).)

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PRKDC, Monoclonal Antibody (Cat# AAA26620)

Full Name

PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide, DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350) (PE)

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Detection of human VISTA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used for IP at 20 ul/mg lysate. VISTA was also immunoprecipitated by a previous lot of this antibody (AAA23805 lot 2). For blotting immunoprecipitated VISTA, AAA23805 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human VISTA by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human VISTA in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-VISTA recombinant monoclonal (AAA23805) (right) or isotype control (left). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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VISTA, Monoclonal Recombinant Antibody (Cat# AAA23805)

Full Name

Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]

Gene Names

C10orf54; B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha

Reactivity

Human

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.01 mL $640/0.1 mL $930/5x0.01 mL $1,235/2x0.1 mL $1,800/3x0.1 mL $2,330/4x0.1 mL $2,635/5x0.1 mL

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WB (Western Blot) (BPNT1 monoclonal antibody Western Blot analysis of BPNT1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged BPNT1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BPNT1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BPNT1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of BPNT1 expression in transfected 293T cell line by BPNT1 monoclonal antibody.Lane 1: BPNT1 transfected lysate (28.1kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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BPNT1, Monoclonal Antibody (Cat# AAA24061)

Full Name

BPNT1 (3'(2'),5'-bisphosphate nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol 1,4-phosphatase)

Gene Names

BPNT1; PIP

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

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WB (Western Blot) (CCNH monoclonal antibody, Western Blot analysis of CCNH expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CCNH is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody. Lane 1: CCNH transfected lysate (37.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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CCNH, Monoclonal Antibody (Cat# AAA25341)

Full Name

CCNH (Cyclin H, Cyclin-H, MO15-associated Protein, p34, p37) (HRP)

Gene Names

CCNH; CAK; p34; p37; CycH

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)

WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SRPK1, Monoclonal Antibody (Cat# AAA25853)

Full Name

SRPK1 (SRSF Protein Kinase 1, SFRS Protein Kinase 1, Serine/Arginine-rich Protein-specific Kinase 1, SR-protein-specific Kinase 1) (PE)

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

IHC (Immunohistochemistry) (IHC staining of human cerebellar neurons using AAA14700.)

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SNAP 25, 26-27kD, Monoclonal Antibody (Cat# AAA14700)

Full Name

SNAP 25, 26-27kD (Synaptosomal-associated Protein)

Reactivity

Rat, Hamster Gerbil and Porcine

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified by Protein A affinity chromatography from tissue culture supernatent

Pricing

$660/0.1 mg $1,000/0.2 mg $1,940/2x0.2 mg $2,825/3x0.2 mg $3,660/4x0.2 mg $4,280/5x0.2 mg

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HepG2 cells using anti-CPSF6 antibody (AAA19714).Overlay histogram showing HepG2 cells stained with AAA19714 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CPSF6 Antibody (AAA19714, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).CPSF6 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CPSF6 Antibody (AAA19714) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CPSF6 using anti-CPSF6 antibody (AAA19714).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: monkey COS-7 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat thymus tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CPSF6 antigen affinity purified monoclonal antibody (#AAA19714) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 59 kDa.)

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CPSF6, Monoclonal Antibody (Cat# AAA19714)

Full Name

Anti-CPSF6 Antibody Picoband (monoclonal, 3F11E1)

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Figure 8. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: rat RH35 whole cell lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19718) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U937 cells using anti-Cyclophilin E/PPIE antibody (AAA19718).Overlay histogram showing U937 cells stained with AAA19718 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19718, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-Cyclophilin E/PPIE antibody (AAA19718).Overlay histogram showing JK cells stained with AAA19718 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19718, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19718) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19718).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19718) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)

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Cyclophilin E/PPIE, Monoclonal Antibody (Cat# AAA19718)

Full Name

Anti-Cyclophilin E/PPIE Antibody Picoband (monoclonal, 7F2)

Gene Names

PPIE; CYP33; CYP-33

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-NDFIP2 antibody (AAA19719).Overlay histogram showing JK cells stained with AAA19719 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NDFIP2 Antibody (AAA19719, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).NDFIP2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-NDFIP2 Antibody (AAA19719) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).NDFIP2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NDFIP2 Antibody (AAA19719) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).NDFIP2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NDFIP2 Antibody (AAA19719) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).NDFIP2 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NDFIP2 Antibody (AAA19719) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).NDFIP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NDFIP2 Antibody (AAA19719) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of NDFIP2 using anti-NDFIP2 antibody (AAA19719).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NDFIP2 antigen affinity purified monoclonal antibody (#AAA19719) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDFIP2 at approximately 39 kDa. The expected band size for NDFIP2 is at 36 kDa.)

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NDFIP2, Monoclonal Antibody (Cat# AAA19719)

Full Name

Anti-NDFIP2 Antibody Picoband (monoclonal, 10D6D7)

Gene Names

NDFIP2; N4WBP5A

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of SiHa cells using anti-Histone H1.0/H1F0 antibody (AAA19720).Overlay histogram showing SiHa cells stained with AAA19720 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Histone H1.0/H1F0 Antibody (AAA19720, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human Hodgkin's lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human differentiated adenocarcinoma of the rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Histone H1.0/H1F0 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Histone H1.0/H1F0 Antibody (AAA19720) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (AAA19720).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U20S whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Histone H1.0/H1F0 antigen affinity purified monoclonal antibody (#AAA19720) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Histone H1.0/H1F0 at approximately 24 kDa. The expected band size for Histone H1.0/H1F0 is at 24 kDa.)

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Histone H1.0/H1F0, Monoclonal Antibody (Cat# AAA19720)

Full Name

Anti-Histone H1.0/H1F0 Antibody Picoband (monoclonal, 5I3E6)

Gene Names

H1F0; H10; H1FV

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of RAW264.7 cells using anti-LSM8 antibody (AAA19721).Overlay histogram showing RAW264.7 cells stained with AAA19721 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody (AAA19721, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of RH35 cells using anti-LSM8 antibody (AAA19721).Overlay histogram showing RH35 cells stained with AAA19721 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody (AAA19721, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-LSM8 antibody (AAA19721).Overlay histogram showing JK cells stained with AAA19721 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody (AAA19721, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of LSM8 using anti-LSM8 antibody (AAA19721).LSM8 was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-LSM8 Antibody (AAA19721) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of LSM8 using anti-LSM8 antibody (AAA19721).LSM8 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-LSM8 Antibody (AAA19721) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of LSM8 using anti-LSM8 antibody (AAA19721).LSM8 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-LSM8 Antibody (AAA19721) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of LSM8 using anti-LSM8 antibody (AAA19721).LSM8 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-LSM8 Antibody (AAA19721) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of LSM8 using anti-LSM8 antibody (AAA19721).LSM8 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-LSM8 Antibody (AAA19721) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of LSM8 using anti-LSM8 antibody (AAA19721).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human THP-1 whole lysates,Lane 4: human K562 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat pancrease tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse pancrease tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-LSM8 antigen affinity purified monoclonal antibody (#AAA19721) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LSM8 at approximately 16 kDa. The expected band size for LSM8 is at 11 kDa.)

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LSM8, Monoclonal Antibody (Cat# AAA19721)

Full Name

Anti-LSM8 Antibody Picoband (monoclonal, 6B11)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-Neurofibromin/NF1 antibody (AAA19654).Overlay histogram showing HepG2 cells stained with AAA19654 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Neurofibromin/NF1 Antibody (AAA19654, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Neurofibromin/NF1 antigen affinity purified monoclonal antibody (#AAA19654) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurofibromin/NF1 at approximately 319 kDa. The expected band size for Neurofibromin/NF1 is at 319 kDa.)

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Neurofibromin/NF1, Monoclonal Antibody (Cat# AAA19654)

Full Name

Anti-Neurofibromin/NF1 Antibody Picoband (monoclonal, 4C6F10)

Gene Names

NF1; WSS; NFNS; VRNF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

FCM (Flow Cytometry)

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GAA, Monoclonal Antibody (Cat# AAA19211)

Full Name

Anti-GAA Antibody (Monoclonal, 2G7)

Gene Names

GAA; LYAG

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: HumanLiver Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Glioma Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Kidney Tissue;Primary Ab: 20ug/ml Mouse Anti-Human ALT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot;Sample:Lane 1: Rat Liver lysate;Lane 2: Rat Skeletal muscle lysatePrimary Ab: 2ug/ml Mouse Anti-Human ALT AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Recombinant ALT, Human.)

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Alanine Aminotransferase, Monoclonal Antibody (Cat# AAA20835)

Full Name

Monoclonal Antibody to Alanine Aminotransferase (ALT)

Gene Names

GPT; AAT1; ALT1; GPT1

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$220/0.1 mL $270/0.2 mL $425/0.5 mL $510/1 mL $1,355/5 mL $2,630/2x5 mL $3,835/3x5 mL $4,975/4x5 mL $6,065/5x5 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-MPI antibody (AAA19349).Overlay histogram showing U87 cells stained with AAA19349 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MPI Antibody (AAA19349, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of MPI using anti- MPI antibody (AAA19349).MPI was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- MPI Antibody (AAA19349) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MPI using anti-MPI antibody (AAA19349).MPI was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MPI Antibody (AAA19349) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MPI using anti-MPI antibody (AAA19349).MPI was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MPI Antibody (AAA19349) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of MPI using anti-MPI antibody (AAA19349).MPI was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MPI Antibody (AAA19349) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MPI using anti-MPI antibody (AAA19349).MPI was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MPI Antibody (AAA19349) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MPI using anti-MPI antibody (AAA19349).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: rat brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MPI antigen affinity purified monoclonal antibody (Catalog # AAA19349) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for MPI at approximately 45KD. The expected band size for MPI is at 45KD.)

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MPI, Monoclonal Antibody (Cat# AAA19349)

Full Name

Anti-MPI Antibody (monoclonal, 5G5)

Gene Names

MPI; PMI; PMI1; CDG1B

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-CDC45L antibody (AAA19360).Overlay histogram showing 293T cells stained with AAA19360 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CDC45L Antibody (AAA19360, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CDC45L using anti-CDC45L antibody (AAA19360).CDC45L was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CDC45L Antibody (AAA19360) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CDC45L using anti-CDC45L antibody (AAA19360).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human HL-60 whole cell lysatesLane 3: human HEK293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- CDC45L antigen affinity purified monoclonal antibody (Catalog # AAA19360) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.)

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CDC45L, Monoclonal Antibody (Cat# AAA19360)

Full Name

Anti-CDC45L Antibody (monoclonal, 6H6)

Gene Names

CDC45; CDC45L; CDC45L2; PORC-PI-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-Ku70 antibody (AAA19362).Overlay histogram showing THP-1 cells stained with AAA19362 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ku70 Antibody (AAA19362, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of Ku70 using anti- Ku70 antibody (AAA19362).Ku70 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Ku70 Antibody (AAA19362) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Ku70 using anti-Ku70 antibody (AAA19362).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Ku70 antigen affinity purified monoclonal antibody (Catalog # AAA19362) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Ku70 at approximately 70KD. The expected band size for Ku70 is at 70KD.)

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Ku70, Monoclonal Antibody (Cat# AAA19362)

Full Name

Anti-Ku70 Antibody (monoclonal, 3D7)

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HEPG2 cells using anti- Liver Carboxylesterase 1/CES1 antibody (AAA19363).Overlay histogram showing HEPG2 cells stained with AAA19363 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of Liver Carboxylesterase 1/CES1 using anti- Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: rat liver tissue lysatesLane 4: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-Liver Carboxylesterase 1/CES1 antigen affinity purified monoclonal antibody (Catalog # AAA19363) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Liver Carboxylesterase 1/CES1 at approximately 63KD. The expected band size for Liver Carboxylesterase 1/CES1 is at 63KD.)

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Liver Carboxylesterase 1/CES1, Monoclonal Antibody (Cat# AAA19363)

Full Name

Anti-Liver Carboxylesterase 1/CES1 Antibody (monoclonal, 3F10)

Gene Names

CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-INPPL1 antibody (AAA19364).Overlay histogram showing THP-1 cells stained with AAA19364 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- INPPL1 Antibody (AAA19364, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of INPPL1 using anti- INPPL1 antibody (AAA19364).INPPL1 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- INPPL1 Antibody (AAA19364) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: rat C6 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- INPPL1 antigen affinity purified monoclonal antibody (Catalog # AAA19364) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 150KD. The expected band size for INPPL1 is at 150KD.)

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INPPL1, Monoclonal Antibody (Cat# AAA19364)

Full Name

Anti-INPPL1 Antibody (monoclonal, 8C13)

Gene Names

INPPL1; OPSMD; SHIP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U937 cells using anti-PP2A-alpha/PPP2CA antibody (AAA19366).Overlay histogram showing U937 cells stained with AAA19366 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PP2A-alpha/PPP2CA Antibody (AAA19366, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of PP2A-alpha/PPP2CA using anti- PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL mouse anti- PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: monkey COS-7 whole cell lysatesLane 4: rat PC-12 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PP2A-alpha/PPP2CA antigen affinity purified monoclonal antibody (Catalog # AAA19366) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PP2A-alpha/PPP2CA at approximately 36KD. The expected band size for PP2A-alpha/PPP2CA is at 36KD.)

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PP2A-alpha/PPP2CA, Monoclonal Antibody (Cat# AAA19366)

Full Name

Anti-PP2A-alpha/PPP2CA Antibody (monoclonal, 3B6)

Gene Names

PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of A549 cells using anti-Transketolase/TKT antibody (AAA19367).Overlay histogram showing A549 cells stained with AAA19367 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Transketolase/TKT Antibody (AAA19367, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 11. IF analysis of Transketolase/TKT using anti- Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Transketolase/TKT was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Transketolase/TKT Antibody (AAA19367) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (AAA19367).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human SW620 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat RH35 whole cell lysatesLane 7: mouse liver tissue lysatesLane 8: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Transketolase/TKT antigen affinity purified monoclonal antibody (Catalog # AAA19367) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Transketolase/TKT at approximately 68KD. The expected band size for Transketolase/TKT is at 68KD.)

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Transketolase/TKT, Monoclonal Antibody (Cat# AAA19367)

Full Name

Anti-Transketolase/TKT Antibody (monoclonal, 3E5)

Gene Names

TKT; TK; TKT1; HEL107

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19369).Overlay histogram showing SiHa cells stained with AAA19369 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19369, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ASS1 using anti- ASS1 antibody (AAA19369).ASS1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- ASS1 Antibody (AAA19369) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19369).ASS1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19369) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19369).ASS1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19369) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19369).ASS1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19369) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19369).ASS1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19369) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19369).ASS1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19369) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ASS1 using anti-ASS1 antibody (AAA19369).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Hek293 wohle cell lysatesLane 4: monkey kidney tissue lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse liver tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-ASS1 antigen affinity purified monoclonal antibody (Catalog # AAA19369) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.)

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ASS1, Monoclonal Antibody (Cat# AAA19369)

Full Name

Anti-ASS1 Antibody (monoclonal, 5I5)

Gene Names

ASS1; ASS; CTLN1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19370).Overlay histogram showing SiHa cells stained with AAA19370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ASS1 using anti- ASS1 antibody (AAA19370).ASS1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- ASS1 Antibody (AAA19370) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ASS1 using anti-ASS1 antibody (AAA19370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Hek293 wohle cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: human T47D whole cell lysatesLane 6: monkey kidney tissue lysatesLane 7: rat liver tissue lysatesLane 8: rat kidney tissue lysatesLane 9: mouse liver tissue lysatesLane 10: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-ASS1 antigen affinity purified monoclonal antibody (Catalog # AAA19370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.)

Compare (5 max)

ASS1, Monoclonal Antibody (Cat# AAA19370)

Full Name

Anti-ASS1 Antibody (monoclonal, 7I9)

Gene Names

ASS1; ASS; CTLN1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: monkey kidney tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-CD13/ANPEP antigen affinity purified monoclonal antibody (Catalog # AAA19371) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CD13/ANPEP at approximately 150KD. The expected band size for CD13/ANPEP is at 150KD.)

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CD13/ANPEP, Monoclonal Antibody (Cat# AAA19371)

Full Name

Anti-CD13/ANPEP Antibody (monoclonal, 5B9)

Gene Names

ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

Reactivity

Human, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 9. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human poison impregnated thyroid gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (#AAA19701) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 18 kDa. The expected band size for PC4/SUB1 is at 18 kDa.)

Compare (5 max)

PC4/SUB1, Monoclonal Antibody (Cat# AAA19701)

Full Name

Anti-PC4/SUB1 Antibody Picoband (monoclonal, 6B5B10)

Gene Names

SUB1; P15; PC4; p14

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of SiHa cells using anti-PDIA6 antibody (AAA19710).Overlay histogram showing SiHa cells stained with AAA19710 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDIA6 Antibody (AAA19710, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).PDIA6 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PDIA6 Antibody (AAA19710) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (AAA19710).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human HT1080 lysates,Lane 4: monkey COS-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDIA6 antigen affinity purified monoclonal antibody (#AAA19710) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 48 kDa. The expected band size for PDIA6 is at 48 kDa.)

Compare (5 max)

PDIA6, Monoclonal Antibody (Cat# AAA19710)

Full Name

Anti-PDIA6 Antibody Picoband (monoclonal, 3H5E7)

Gene Names

PDIA6; P5; ERP5; TXNDC7

Reactivity

Human, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Figure 10. IF analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing U87 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of RH35 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing RH35 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of ANA-1 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing ANA-1 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human tonsli tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Hek293 whole cell lysates,Lane 3: human U87 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: rat spleen tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EIF4A1 antigen affinity purified monoclonal antibody (#AAA19711) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF4A1 at approximately 47 kDa. The expected band size for EIF4A1 is at 47kDa.)

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EIF4A1, Monoclonal Antibody (Cat# AAA19711)

Full Name

Anti-EIF4A1 Antibody Picoband (monoclonal, 3F11)

Gene Names

EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Figure 8. IF analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 7. IF analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD55 using anti-CD55 antibody (AAA19674).CD55 was detected in a paraffin-embedded section of human cladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD55 Antibody (AAA19674) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (AAA19674).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (#AAA19674) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa.)

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CD55, Monoclonal Antibody (Cat# AAA19674)

Full Name

Anti-CD55 Antibody Picoband (monoclonal, 5B9E1)

Gene Names

CD55; CR; TC; DAF; CROM

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Figure 12. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (M00179-1) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (M00179-1) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of C6 cells using anti-splicing factor 1 antibody (AAA19676).Overlay histogram showing C6 cells stained with AAA19676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19676, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Neuro-2a cells using anti-splicing factor 1 antibody (AAA19676).Overlay histogram showing Neuro-2a cells stained with AAA19676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19676, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-splicing factor 1 antibody (AAA19676).Overlay histogram showing A431 cells stained with AAA19676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19676, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of human squamous metaplasia of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).splicing factor 1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19676) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19676).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SKOV-3 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse ovary tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-splicing factor 1 antigen affinity purified monoclonal antibody (#AAA19676) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for splicing factor 1 at approximately 68 kDa. The expected band size for splicing factor 1 is at 68 kDa.)

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splicing factor 1, Monoclonal Antibody (Cat# AAA19676)

Full Name

Anti-splicing factor 1 Antibody Picoband (monoclonal, 7D9E3)

Gene Names

SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.