Mouse anti-Human Adipophilin/Perilipin-2 Monoclonal Antibody | anti-PLIN2 antibody

Adipophilin/Perilipin-2 (Marker of Obesity)

Cat. Num. AAA62936

• Gene Names: PLIN2; ADFP; ADRP
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Adipophilin/Perilipin-2, Antibody; Adipophilin/Perilipin-2 (Marker of Obesity); Adipophilin; ADFP; Adipose differentiation-related protein (ADRP); Perilipin-2 (PLIN2); anti-PLIN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ADFP/1365
• Specificity: Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 437

• Applicable Applications for anti-PLIN2 antibody: WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 9p22.1
• Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Adipophilin Mouse Monoclonal Antibody (ADFP/1365).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM/FACS (Flow Cytometry)

(Flow Cytometry of human Adipophilin on PBMC. Green: Isotype Control; Red: Adipophilin Monoclonal Antibody (ADFP/1365).)

SDS-PAGE

(SDS-PAGE Analysis Purified Adipophilin Mouse Monoclonal Antibody (ADFP/1365). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot of recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).)

References

Kern, P.A., et al. 2004. Perilipin expression in human adipose tissue is elevated with obesity. J. Clin. Endocrinol. Metab. 89: 1352-1358.

NCBI and Uniprot Product Information

• NCBI GI #: 34577059
• NCBI GeneID: 123
• NCBI Accession #: NP_001113.2
• NCBI GenBank Nucleotide #: NM_001122.3
• UniProt Accession #: Q99541; Q9BSC3
• Molecular Weight: 48kDa
• NCBI Official Full Name: perilipin-2
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP
• NCBI Protein Information: perilipin-2
• UniProt Protein Name: Perilipin-2
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP; ADRP